FastQCFastQC Report
Mon 4 Feb 2019
000000000-BTF83_l01_n02_Exp1_SrcID3_Shed_Ind2_D1_rep2_SID23.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BTF83_l01_n02_Exp1_SrcID3_Shed_Ind2_D1_rep2_SID23.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences93229
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA1700.1823466946980017No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG1630.1748383013869075No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT1140.12227954820924819No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA1010.10833538920293041No Hit
GAGCAAAAGCAGGGGATAATTCTATTAATCATGAAGACCATCATTGCTTT980.10511750635531862No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTGAT259.359869E-487.07
GAGCGAA451.2241017E-680.555561
GAGCAAA2050.067.195121
GCAAAAG2550.065.392163
CAAAAGC2650.062.924534
GTGATGA350.00355636762.1428579
GCAGGGT1300.061.3461579
CGAAAGC954.4929038E-1061.0526283
AAGCAGG3350.060.597017
TAGGAGG606.757451E-660.4166684
CTAGGAG502.0007571E-458.03
AGCAGGT1500.058.08
AGCAGGG1800.056.388898
AGCGAAA1051.0895747E-955.2380941
TATGATA400.006033751654.3752
TATATAG701.6840444E-551.7857135
AGGAGGT604.921881E-448.3333325
GGGTAGT450.00961194548.3333321
TGGTGAG450.00961194548.3333327
TGGTATC450.00961194548.3333325
Produced by FastQC (version 0.11.5)