FastQCFastQC Report
Mon 4 Feb 2019
000000000-BTF83_l01_n02_Exp1_SrcID3_Shed_Ind2_D1_rep1_SID3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BTF83_l01_n02_Exp1_SrcID3_Shed_Ind2_D1_rep1_SID3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences138354
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA4460.32236147852609975No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG4090.2956184859129479No Hit
GAGCAAAAGCAGGGGATAATTCTATTAATCATGAAGACCATCATTGCTTT2580.18647816470792317No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT2130.1539529034216575No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA1990.14383393324370816No Hit
GAGCAAAAGCAGGGTAGATAATCACTCACTGAGTGACATCAAAATCATGG1950.14094279890715122No Hit
CTCTAATATTGCTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAA1760.12720991080850572No Hit
CCTGTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGAT1710.12359599288780954No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT1700.12287320930367028No Hit
GTATACATCATGGTCATAAGTCCCATTTCTGATTGACTCTATGCAAGCGT1590.11492258987813868No Hit
GTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGATGTT1520.10986310478916404No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGATCCCCGTTCCC1490.10769475403674632No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC1450.10480361970018938No Hit
AAACAAGGGTGTTTTTTATTATTAAATAAGCTGAAACGAGAAAGTTCTTA1420.10263526894777165No Hit
GTGTTATCATTCCATTCAAGTCCCCCGATGAGGACTCCAACTGCATTTTT1410.10191248536363241No Hit
ATATACAACAGGATGGGGGCTGTGACCACTGAAGTGGCATTTGGCCTGGT1390.10046691819535394No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGTAG100.0070843073145.02
GAGCAAA4700.080.212771
GCAGGGT2350.074.042569
GCAAAAG5200.072.53
AAGCAGG5650.071.858417
CAAAAGC5350.070.467294
AGCAGGG3100.065.483878
CTTATAT451.1902392E-464.444443
GCAGGAG451.1902392E-464.444449
TATAGGC451.1902392E-464.444447
AGCAGGC451.1902392E-464.444448
TATATAG1051.6370905E-1162.1428575
CCTCGAG350.003560012762.1428579
AGCTTAT502.0041683E-458.01
TTATATA502.0041683E-458.04
AGCAAAA6550.057.5572552
GAGCGAA651.0886995E-555.769231
AGGGTTA400.006039915654.375145
ACCTCGA400.006039915654.3758
CTCAGCG400.006039915654.3759
Produced by FastQC (version 0.11.5)