FastQCFastQC Report
Mon 4 Feb 2019
000000000-BTF83_l01_n02_Exp1_SrcID2_Shed_Ind1_D1_rep2_SID22.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BTF83_l01_n02_Exp1_SrcID2_Shed_Ind1_D1_rep2_SID22.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences113082
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAACAAGGGTGTTTTTTATTATTAAATAAGCTGAAACGAGAAAGTTCTTA1810.16006084080578695No Hit
GTGTTATCATTCCATTCAAGTCCCCCGATGAGGACTCCAACTGCATTTTT1620.14325887409136734No Hit
CTCTAATATTGCTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAA1490.1317627916025539No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT1450.12822553545214976No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA1360.12026670911374046No Hit
TGATAACACAGTTCGAGTCTCTGAAACTCTACAGAGATTCGCTTGGAGAA1360.12026670911374046No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT1250.1105392547001291No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTGAT350.003558328862.1428537
GATAATT502.002592E-458.02
ACAAGGT502.002592E-458.03
AGGTAGT400.00603706854.3756
CTACTAA701.6863522E-551.785716
TCTACTA858.8539855E-751.176475
AAGGGTG1509.094947E-1248.3333365
GTAGTAA752.5361302E-548.3333361
ATAATTC604.9263664E-448.3333323
TAAACTA604.9263664E-448.3333325
CAAGGTA450.00961720648.3333324
AGTCTGC450.00961720648.3333328
TAATGAC604.9263664E-448.3333325
CAAGGGT1602.0008883E-1145.31254
TATAGGA1602.0008883E-1145.31252
TAGGAAG1602.0008883E-1145.31254
CGAAAGC657.3088985E-444.6153833
AGCGGTC657.3088985E-444.6153839
GGTGTTT1652.7284841E-1143.9393928
GTATAGG1652.7284841E-1143.9393921
Produced by FastQC (version 0.11.5)