FastQCFastQC Report
Mon 4 Feb 2019
000000000-BTF83_l01_n02_Exp1_SrcID2_Shed_Ind1_D1_rep1_SID2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BTF83_l01_n02_Exp1_SrcID2_Shed_Ind1_D1_rep1_SID2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences135297
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT2440.18034398397599355No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA2100.15521408456950264No Hit
CTCTAATATTGCTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAA1810.13377975860514277No Hit
TCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGATCCCCGTTCCCA1800.13304064391671658No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG1780.13156241453986414No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT1760.13008418516301173No Hit
GTGTTATCATTCCATTCAAGTCCCCCGATGAGGACTCCAACTGCATTTTT1650.1219539235903235No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGATCCCCGTTCCC1600.11825835014819248No Hit
AGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTCAATCC1560.11530189139448768No Hit
AAACAAGGGTGTTTTTTATTATTAAATAAGCTGAAACGAGAAAGTTCTTA1540.11382366201763527No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC1530.11308454732920907No Hit
ATATACAACAGGATGGGGGCTGTGACCACTGAAGTGGCATTTGGCCTGGT1520.11234543264078288No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA1520.11234543264078288No Hit
ATACATGATAGAATCCCTCATCGAACCCTATTAATGAATGAGTTGGGTGT1420.10495428575652084No Hit
ATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTATGA1370.10125871231438982No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA458.367351E-11112.777781
GGCGTCT606.7739293E-660.4166687
GAAGTAT502.0040068E-458.07
TAGATAA902.0318112E-856.388892
TTAGGCG651.0885682E-555.769234
AGGCGTC651.0885682E-555.769236
GCAGGGT1051.0968506E-955.2380949
AGCGAAA953.112291E-853.4210552
TATACTA953.112291E-853.4210555
GTATTAG701.688132E-551.785711
TAGGCGT701.688132E-551.785715
GTGTTTT1351.8735591E-1048.3333369
CTATGCT752.5387999E-548.3333369
GCGTCTC752.5387999E-548.3333368
TTATATT450.00962126248.3333322
CTAGTAG604.9298274E-448.3333323
TACTATC901.3150275E-648.3333327
TAGTGGT604.9298274E-448.3333324
ATATACT1255.138645E-946.3999984
AAGCAGG3200.045.31257
Produced by FastQC (version 0.11.5)