FastQCFastQC Report
Mon 4 Feb 2019
000000000-BTF83_l01_n02_Exp1_SrcID25_NL_Cnt6_D3_rep2_SID40.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BTF83_l01_n02_Exp1_SrcID25_NL_Cnt6_D3_rep2_SID40.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences156179
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG7600.4866211206372176No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT5400.3457571120317072No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA5310.33999449349784544No Hit
AGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTCAATCC4050.2593178340237804No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTT4020.2573969611791598No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT3560.22794357756164402No Hit
GTGTTATCATTCCATTCAAGTCCCCCGATGAGGACTCCAACTGCATTTTT3000.19208728446205955No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC2960.18952612066923208No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT2880.18440379308357718No Hit
GTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTA2850.1824829202389566No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA2770.17736059265330167No Hit
CTCTAATATTGCTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAA2770.17736059265330167No Hit
GTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGATGTT2670.170957683171233No Hit
AAACAAGGGTGTTTTTTATTATTAAATAAGCTGAAACGAGAAAGTTCTTA2580.16519506463737121No Hit
GAGCAAAAGCAGGGTAGATAATCACTCACTGAGTGACATCAAAATCATGG2530.1619936098963369No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAA2520.16135331894813004No Hit
TGATAACACAGTTCGAGTCTCTGAAACTCTACAGAGATTCGCTTGGAGAA2510.16071302799992318No Hit
CCTGTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGAT2300.147266918087579No Hit
GCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAGCT2030.1299790624859936No Hit
GAAGCAGTTTTCTTGATTCAGCTGAAAATCCTTCTAGTTGTGGAGATGCA2010.1286984805895799No Hit
ATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTATGA1960.12549702584854558No Hit
AGCGAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAGC1850.11845382541827007No Hit
GTAGTAAACAGTATTTGCAACACTACAGGGGCTGAGAAACCAAAGTTTCT1840.11781353447006321No Hit
ATATACAACAGGATGGGGGCTGTGACCACTGAAGTGGCATTTGGCCTGGT1830.11717324352185632No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGATCCCCGTTCCC1800.11525237067723573No Hit
CTCCAGCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCACTCT1740.11141062498799455No Hit
GATGTAAGGCTTGCATAAATGTTATTTGCTCAAAACTATTCTCTGTTATC1740.11141062498799455No Hit
GTCGTACTCCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCA1590.10180626076489158No Hit
GACATAATGGATCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCT1590.10180626076489158No Hit
GAGCAAAAGCAGGGGATAATTCTATTAATCATGAAGACCATCATTGCTTT1580.10116596981668471No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTCGTG100.0070851753144.99998145
GAGCGAA750.0135.333331
AGCAGGC850.093.823538
TTTCCTA406.093178E-790.62499145
TTAGTAG259.371814E-487.02
AGCGAAA1200.084.5833362
GCAAAAG5000.079.753
CGAAAGC1350.075.185194
CAAAAGC5350.074.5327154
GAGCGGT406.6456094E-572.499998
GCAGGAG406.6456094E-572.499999
GAGCAAA5800.071.251
AAGCAGG7350.068.0612267
GCAGGGT2950.066.3559349
CACATCG554.045587E-665.909096145
ATTAAGA451.1907178E-464.444443
GACCCCG451.1907178E-464.44444145
AGAGCGG451.1907178E-464.444447
ATTAGTA350.003560870762.1428571
GCTATCC350.003560870762.1428573
Produced by FastQC (version 0.11.5)