FastQCFastQC Report
Mon 4 Feb 2019
000000000-BTF83_l01_n02_Exp1_SrcID25_NL_Cnt6_D3_rep1_SID20.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BTF83_l01_n02_Exp1_SrcID25_NL_Cnt6_D3_rep1_SID20.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63034
Sequences flagged as poor quality0
Sequence length151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG1140.18085477678713074No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA830.1316749690643145No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT740.11739696037059366No Hit
AGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTCAATCC720.1142240695497668No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTTATG259.3456806E-487.01
CAAAAGC1900.068.684214
GAGCAAA1900.064.868421
AGAGTCT451.1852285E-464.444446
ATCTAAG350.003551015962.1428531
TGTATAT702.2992936E-762.1428533
AGTATTA350.003551015962.1428538
GCAAAAG2000.061.6253
AGCGAAA606.7321E-660.4166681
TTAGAGT501.9957549E-458.04
AAGCAGG2650.054.7169847
GTATTAA400.006024705754.3759
GAGTATT400.006024705754.3757
TCTAAGA400.006024705754.3752
GTATATA400.006024705754.3754
AGTAGAA1205.820766E-1154.3751
ATATTAG400.006024705754.3751
GCAGGGT1353.6379788E-1253.70379
ACAAGGG553.1958678E-452.7272728
CGAAAGC701.6777576E-551.785713
Produced by FastQC (version 0.11.5)