FastQCFastQC Report
Mon 4 Feb 2019
000000000-BTF83_l01_n02_Exp1_SrcID24_NL_Cnt5_D3_rep2_SID39.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BTF83_l01_n02_Exp1_SrcID24_NL_Cnt5_D3_rep2_SID39.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences154565
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG3960.2562028919871899No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA3230.20897357098955133No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT2910.18827030699058647No Hit
AGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTCAATCC2880.18632937599068353No Hit
GAGCAAAAGCAGGGTAGATAATCACTCACTGAGTGACATCAAAATCATGG2450.15850936499207455No Hit
CCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATGTCAAAG2220.14362889399281856No Hit
GTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGATGTT2070.1339242389933038No Hit
GTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTA2030.13133633099343317No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT2010.13004237699349788No Hit
CCTGTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGAT1980.12810144599359494No Hit
CTCTAATATTGCTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAA1910.12357260699382137No Hit
ATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTATGA1900.1229256299938537No Hit
GTGTTATCATTCCATTCAAGTCCCCCGATGAGGACTCCAACTGCATTTTT1850.11969074499401547No Hit
GTCGTACTCCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCA1800.11645585999417721No Hit
TTCAATGGGAATACAGAGGGAAGAACATCTGACATGAGGACCGAAATCAT1780.1151619059942419No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT1780.1151619059942419No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTT1760.1138679519943066No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC1750.11322097499433895No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA1740.11257399799437129No Hit
TTCAAAATGAGTTTAACAAGGCATGCGAACTGACAGATTCAAGCTGGATA1720.111280043994436No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAA1630.10545725099472714No Hit
TGATAACACAGTTCGAGTCTCTGAAACTCTACAGAGATTCGCTTGGAGAA1620.10481027399475949No Hit
AAACAAGGGTGTTTTTTATTATTAAATAAGCTGAAACGAGAAAGTTCTTA1570.10157538899492123No Hit
GTATACATCATGGTCATAAGTCCCATTTCTGATTGACTCTATGCAAGCGT1570.10157538899492123No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGC100.0070851045145.0145
GTGCTAT100.0070851045145.0145
GAGCGAA406.092869E-790.6251
AGCGAAA1000.079.751
CAAGGTA606.778493E-660.4166688
TCGTACT606.778493E-660.4166682
AAGGTAC502.0049066E-458.09
CGAAAGC1400.056.9642873
GCAAAAG3700.056.8243263
ACAAGGT902.0343577E-856.3888857
GCAGGGT1550.056.1290329
TAAACTA553.2104802E-452.7272725
GCCTAAG553.2104802E-452.7272721
GAGCAAA4050.051.913581
CTAACAT604.932027E-448.3333326
CAAAAGC4450.047.247194
GTAGTAA1109.8862074E-846.136361
ATACTAA657.317272E-444.6153833
TACTAAC657.317272E-444.6153834
AAGCAGG5550.044.4144137
Produced by FastQC (version 0.11.5)