Basic Statistics
Measure | Value |
---|---|
Filename | 000000000-BTF83_l01_n02_Exp1_SrcID24_NL_Cnt5_D3_rep1_SID19.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 109627 |
Sequences flagged as poor quality | 0 |
Sequence length | 151 |
%GC | 44 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA | 167 | 0.15233473505614492 | No Hit |
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG | 163 | 0.14868599888713546 | No Hit |
GAGCAAAAGCAGGGTAGATAATCACTCACTGAGTGACATCAAAATCATGG | 145 | 0.1322666861265929 | No Hit |
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT | 140 | 0.1277057659153311 | No Hit |
GTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGATGTT | 137 | 0.124969213788574 | No Hit |
AGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTCAATCC | 127 | 0.11584737336605033 | No Hit |
CCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATGTCAAAG | 113 | 0.10307679677451723 | No Hit |
ATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTATGA | 111 | 0.10125242869001251 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GTGCTGA | 35 | 0.0035580385 | 62.142857 | 6 |
GAGCGAA | 35 | 0.0035580385 | 62.142857 | 1 |
GAGCAAA | 315 | 0.0 | 62.142857 | 1 |
GCAAAAG | 330 | 0.0 | 59.31818 | 3 |
GCAGGGT | 155 | 0.0 | 56.12903 | 9 |
CAAAAGC | 350 | 0.0 | 55.92857 | 4 |
GTATGAC | 65 | 1.08719705E-5 | 55.76923 | 1 |
TAGTGAT | 40 | 0.0060365773 | 54.375 | 7 |
AGCAGGC | 40 | 0.0060365773 | 54.375 | 8 |
AAGCAGG | 405 | 0.0 | 51.91358 | 7 |
AGCAGGG | 225 | 0.0 | 51.555553 | 8 |
GTATATA | 100 | 4.6518835E-8 | 50.75 | 1 |
AGTATCG | 45 | 0.009616427 | 48.333332 | 8 |
TATAGGT | 100 | 2.711815E-6 | 43.5 | 4 |
ATATACA | 185 | 1.8189894E-12 | 43.108105 | 1 |
AGCAAAA | 455 | 0.0 | 43.02198 | 2 |
GCAGGGG | 85 | 5.310303E-5 | 42.64706 | 9 |
AAAGCAG | 530 | 0.0 | 41.037735 | 6 |
TATATAG | 110 | 5.2214873E-6 | 39.545452 | 2 |
ATAGGGT | 75 | 0.0014776242 | 38.666664 | 6 |