FastQCFastQC Report
Mon 4 Feb 2019
000000000-BTF83_l01_n02_Exp1_SrcID24_NL_Cnt5_D3_rep1_SID19.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BTF83_l01_n02_Exp1_SrcID24_NL_Cnt5_D3_rep1_SID19.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences109627
Sequences flagged as poor quality0
Sequence length151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA1670.15233473505614492No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG1630.14868599888713546No Hit
GAGCAAAAGCAGGGTAGATAATCACTCACTGAGTGACATCAAAATCATGG1450.1322666861265929No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT1400.1277057659153311No Hit
GTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGATGTT1370.124969213788574No Hit
AGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTCAATCC1270.11584737336605033No Hit
CCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATGTCAAAG1130.10307679677451723No Hit
ATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTATGA1110.10125242869001251No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCTGA350.003558038562.1428576
GAGCGAA350.003558038562.1428571
GAGCAAA3150.062.1428571
GCAAAAG3300.059.318183
GCAGGGT1550.056.129039
CAAAAGC3500.055.928574
GTATGAC651.08719705E-555.769231
TAGTGAT400.006036577354.3757
AGCAGGC400.006036577354.3758
AAGCAGG4050.051.913587
AGCAGGG2250.051.5555538
GTATATA1004.6518835E-850.751
AGTATCG450.00961642748.3333328
TATAGGT1002.711815E-643.54
ATATACA1851.8189894E-1243.1081051
AGCAAAA4550.043.021982
GCAGGGG855.310303E-542.647069
AAAGCAG5300.041.0377356
TATATAG1105.2214873E-639.5454522
ATAGGGT750.001477624238.6666646