FastQCFastQC Report
Mon 4 Feb 2019
000000000-BTF83_l01_n02_Exp1_SrcID23_NL_Cnt4_D3_rep1_SID18.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BTF83_l01_n02_Exp1_SrcID23_NL_Cnt4_D3_rep1_SID18.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences125721
Sequences flagged as poor quality0
Sequence length151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA2290.18214936247723132No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT1320.1049943923449543No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG1270.10101733202885754No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTAGTA203.8581117E-4108.751
GAGCGAA502.2945133E-672.51
GCAGGGT1150.069.347839
GTGTCTG350.003559255562.1428579
GGGTTTA350.003559255562.142857145
TTAGTAT350.003559255562.1428572
TAGTAGA350.003559255562.1428574
GCAAAAG3050.057.0491833
GTAGGTG651.0881229E-555.769239
AAGCAGG4100.054.8170747
CTAGTAG400.00603863454.3753
TGGGGTG400.00603863454.3755
AGCGAAA953.1099262E-853.4210471
CAAAAGC3400.053.3088264
GTCTAGT553.2081688E-452.7272721
AGCAGGG2250.051.5555538
TAGGTCC901.3142799E-648.3333369
GCAGGAG450.00961969148.3333369
CGAAAGC1056.841947E-848.3333323
GAGCAAA3450.048.3333321
Produced by FastQC (version 0.11.5)