FastQCFastQC Report
Mon 4 Feb 2019
000000000-BTF83_l01_n02_Exp1_SrcID22_NL_Cnt3_D3_rep2_SID37.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BTF83_l01_n02_Exp1_SrcID22_NL_Cnt3_D3_rep2_SID37.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85411
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTT2590.303239629555912No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG2150.2517240168128227No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA1990.2329910667244266No Hit
GTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGATGTT1510.1767922164592383No Hit
GAGCAAAAGCAGGGTAGATAATCACTCACTGAGTGACATCAAAATCATGG1460.17093816955661448No Hit
GAGCAAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAA1300.15220521946821838No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT1260.14752198194611937No Hit
AGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTCAATCC1230.14400955380454508No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT1220.1428387444240203No Hit
ATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTATGA1200.1404971256629708No Hit
GAGCAAAAGCAGGGGATAATTCTATTAATCATGAAGACCATCATTGCTTT1150.13464307876034703No Hit
GGATACACCATGGATACTGTCAACAGGACACATCAGTACTCAGAAAAGGG1140.13347226937982226No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAA1040.1217641755745747No Hit
GTAGTAAACAGTATTTGCAACACTACAGGGGCTGAGAAACCAAAGTTTCT980.11473931929142617No Hit
GTATACATCATGGTCATAAGTCCCATTTCTGATTGACTCTATGCAAGCGT930.10888527238880237No Hit
CCTGTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGAT910.10654365362775285No Hit
ATATGATAGTGATGAACCAGAATTGAGGTCGCTAGCAAGTTGGATTCAGA890.10420203486670335No Hit
CCTATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTA890.10420203486670335No Hit
GTATATGTGAACTTCTCTCCTTGTTACTCCAATTTCGATGAATCTATTCT860.10068960672512908No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAACTA352.7399983E-7103.571435
AGCAGGC750.096.666678
CAAAAGC2950.090.9322054
TATAGGT259.357166E-487.04
GCAAAAG3150.085.158733
GAGCGAA451.2232194E-680.555551
GCAGGGT1100.079.090919
GAGCAAA3500.078.714281
AAGCAGG3850.075.324687
CGAAAGC502.2882014E-672.54
TAGGTCC300.001929664872.56
GCAGGCA1000.072.59
CTATTCG502.2882014E-672.59
GTAACAG300.001929664872.59
TTTTTAA554.029909E-665.9090961
GTGCATG350.003555346762.1428579
GGTAACA350.003555346762.1428578
TATATAG350.003555346762.1428572
ATATAGG350.003555346762.1428573
GTATATA350.003555346762.1428571
Produced by FastQC (version 0.11.5)