FastQCFastQC Report
Mon 4 Feb 2019
000000000-BTF83_l01_n02_Exp1_SrcID22_NL_Cnt3_D3_rep1_SID17.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BTF83_l01_n02_Exp1_SrcID22_NL_Cnt3_D3_rep1_SID17.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences96863
Sequences flagged as poor quality0
Sequence length151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTT2040.21060673322114742No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG1140.11769199797652355No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA1110.11459484013503608No Hit
GTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGATGTT1040.10736813850489868No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT970.10014143687476126No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA554.110916E-1092.2727361
GTATAGG608.1854523E-1084.5833361
AAGGGTG755.4569682E-1177.333335
GCAGGGG702.7775968E-972.59
TATAGGA754.793037E-967.6666642
ACAAGGG807.9835445E-963.43753
AGGGTGT954.4929038E-1061.0526356
AAGCAGG3600.058.4027827
GAAGCTC753.7255995E-758.07
CGAAAGC902.0238076E-856.3888933
AGCGAAA902.0238076E-856.3888931
GCAAAAG2850.055.9649123
ATGTATA651.0862461E-555.769232
GAGCAAA2900.055.01
GGGTCGG400.006034463654.3759
GGCGTCT400.006034463654.3757
AGGGTCG400.006034463654.3758
TAGGAAG805.8182195E-754.3754
AGCAGGC805.8182195E-754.3758
CAAAAGC3000.053.1666644
Produced by FastQC (version 0.11.5)