FastQCFastQC Report
Mon 4 Feb 2019
000000000-BTF83_l01_n02_Exp1_SrcID21_NL_Cnt2_D3_rep2_SID36.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BTF83_l01_n02_Exp1_SrcID21_NL_Cnt2_D3_rep2_SID36.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57884
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG1770.3057839817566167No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA1470.2539561882385461No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTT1280.22113191901043466No Hit
GAGCAAAAGCAGGGTAGATAATCACTCACTGAGTGACATCAAAATCATGG1260.21767673277589664No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT940.16239375302328796No Hit
GTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGATGTT890.15375578743694285No Hit
CCTGTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGAT860.1485730080851358No Hit
CTCTAATATTGCTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAA850.14684541496786677No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC800.13820744938152166No Hit
AGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTCAATCC750.12956948379517655No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT730.12611429756063852No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA720.12438670444336951No Hit
GAGCAAAAGCAGGGGATAATTCTATTAATCATGAAGACCATCATTGCTTT710.12265911132610047No Hit
GGATACACCATGGATACTGTCAACAGGACACATCAGTACTCAGAAAAGGG700.12093151820883145No Hit
GTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTA650.11229355262248635No Hit
ATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTATGA620.1071107732706793No Hit
AAACAAGGGTGTTTTTTATTATTAAATAAGCTGAAACGAGAAAGTTCTTA620.1071107732706793No Hit
ATACATGATAGAATCCCTCATCGAACCCTATTAATGAATGAGTTGGGTGT620.1071107732706793No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT600.10365558703614125No Hit
TGATAACACAGTTCGAGTCTCTGAAACTCTACAGAGATTCGCTTGGAGAA600.10365558703614125No Hit
GTCGTACTCCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCA590.10192799391887224No Hit
GTGTTATCATTCCATTCAAGTCCCCCGATGAGGACTCCAACTGCATTTTT590.10192799391887224No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGAGGT100.0070737256145.04
GTAGGAG100.0070737256145.03
TGTAGGA100.0070737256145.02
TAGACAG203.847469E-4108.755
ACAGACC203.847469E-4108.758
GCAGGAG203.847469E-4108.759
CTACTAG259.3417836E-487.01
GGGTAAC259.3417836E-487.09
GAGCGAA259.3417836E-487.01
GAGCAAA1700.085.294121
AGCAGGC451.2182081E-680.555568
CGAAAGC651.5297701E-978.076923
GCAGGGT851.8189894E-1276.764719
GCAAAAG1950.074.358983
ACTAGAC406.6103035E-572.53
GACAGAC300.001926504572.499997
AGCGAAA607.898416E-872.499991
AAGCAGG2450.068.0612267
AGCAGGG1200.066.458338
CAGACCC451.1844105E-464.444449
Produced by FastQC (version 0.11.5)