FastQCFastQC Report
Mon 4 Feb 2019
000000000-BTF83_l01_n02_Exp1_SrcID21_NL_Cnt2_D3_rep1_SID16.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BTF83_l01_n02_Exp1_SrcID21_NL_Cnt2_D3_rep1_SID16.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences119122
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG2470.2073504474404392No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTT2190.18384513356055138No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA2010.16873457463776634No Hit
GAGCAAAAGCAGGGTAGATAATCACTCACTGAGTGACATCAAAATCATGG1810.15194506472356073No Hit
CTCTAATATTGCTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAA1480.12424237336512148No Hit
ATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTATGA1450.12172394687799064No Hit
GTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGATGTT1430.12004499588657007No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC1370.11500814291230839No Hit
CCTGTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGAT1360.11416866741659812No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT1310.10997128993804671No Hit
GTGTTATCATTCCATTCAAGTCCCCCGATGAGGACTCCAACTGCATTTTT1240.10409496146807475No Hit
AAACAAGGGTGTTTTTTATTATTAAATAAGCTGAAACGAGAAAGTTCTTA1240.10409496146807475No Hit
GTCGTACTCCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCA1230.10325548597236447No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGGGT1650.096.666679
GAGCGAA451.0833901E-896.6666641
TCCAGCG259.366312E-487.0145
GCAAAAG3750.077.3333363
AGCAGGG2650.073.867928
GAGCAAA4000.072.51
AAGCAGG5100.066.813727
ATAGACA350.003558796862.1428574
CAAAAGC4750.061.052634
GTAAGAC651.0877737E-555.769233
AGCAGGC1051.0950316E-955.2380948
TTAGCAC400.00603785854.3753
AAGGGTG953.1081072E-853.4210555
AGCAAAA5850.049.5726472
AGGGTGT901.3136923E-648.3333326
ATAGAAT450.0096184648.3333328
AAAGCAG7100.047.9929586
CACTCGA657.310483E-444.6153836
CGAAAGC1151.3986028E-744.1304363
AAAAGCA6900.043.0797125
Produced by FastQC (version 0.11.5)