FastQCFastQC Report
Mon 4 Feb 2019
000000000-BTF83_l01_n02_Exp1_SrcID20_NL_Cnt1_D3_rep1_SID15.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BTF83_l01_n02_Exp1_SrcID20_NL_Cnt1_D3_rep1_SID15.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences133509
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG3370.25241744002277No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTT2500.1872532937854377No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT2300.17227303028260266No Hit
AGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTCAATCC2300.17227303028260266No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA2280.17077500393231917No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT1900.14231250327693265No Hit
GTGTTATCATTCCATTCAAGTCCCCCGATGAGGACTCCAACTGCATTTTT1790.13407335835037337No Hit
CTCTAATATTGCTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAA1770.1325753320000899No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC1740.13032829247466463No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT1730.12957927929952287No Hit
AAACAAGGGTGTTTTTTATTATTAAATAAGCTGAAACGAGAAAGTTCTTA1720.12883026612438114No Hit
GAGCAAAAGCAGGGTAGATAATCACTCACTGAGTGACATCAAAATCATGG1480.1108539499209791No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAA1370.10261480499441984No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA1350.10111677864413635No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGGAG203.8586426E-4108.757
AGCAGGC750.087.08
GTAGTAC300.001932057872.499991
TAGTACA300.001932057872.499992
CGAAAGC851.6734703E-1068.235293
GAGCAAA4050.068.024691
CAAAAGC3800.064.868424
GCAAAAG3950.064.240513
AGTCAAG350.003559739862.1428573
GAGCGAA350.003559739862.1428571
GCAGGGT1800.060.4166689
AAGCAGG4700.058.6170237
GAGCGGT502.0039125E-458.08
AGCAGGG2350.055.5319188
AGCGAAA1051.0968506E-955.2380941
AAGGGTG1051.0968506E-955.2380945
TCGTACT400.006039452354.3752
AGAGTCT1101.6570993E-952.7272726
AGAGCGG553.208892E-452.7272727
CATATGT701.6880127E-551.7857134
Produced by FastQC (version 0.11.5)