FastQCFastQC Report
Mon 4 Feb 2019
000000000-BTF83_l01_n02_Exp1_SrcID1_SV_IAV_X31_D0_rep2_SID21.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BTF83_l01_n02_Exp1_SrcID1_SV_IAV_X31_D0_rep2_SID21.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences103185
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG2910.28201773513592093No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA2570.2490672093812085No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTT1760.17056742743615835No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT1550.15021563211707128No Hit
AAACAAGGGTGTTTTTTATTATTAAATAAGCTGAAACGAGAAAGTTCTTA1490.14440083345447496No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT1300.12598730435625333No Hit
GTGTTATCATTCCATTCAAGTCCCCCGATGAGGACTCCAACTGCATTTTT1290.1250181712458206No Hit
AGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTCAATCC1270.12307990502495518No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT1240.12017250569365703No Hit
CTCTAATATTGCTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAA1180.11435770703106071No Hit
GAGCAAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAA1170.113388573920628No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC1120.1085429083684644No Hit
GAAGCAGTTTTCTTGATTCAGCTGAAAATCCTTCTAGTTGTGGAGATGCA1040.10078984348500265No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGAGGT100.007081711145.0145
GAGCGAA700.0103.571431
GAGCAAA3200.095.156251
GCAGGGT1600.090.6259
GGTCTCG259.3627267E-487.0145
AGCAGGG2200.085.681828
CAAAAGC4150.073.373494
GCAGGTC607.9602614E-872.499999
AAGCAGG5250.071.8095257
GCAAAAG4600.066.1956563
GCAGGAG606.7625606E-660.416669
CGAAAGC1251.8189894E-1258.04
AGTGCAG502.0017651E-458.08
AGGGGTC400.00603557354.3755
AAAGCAG6800.054.3756
AGCAAAA5900.054.0677952
AGCGAAA1353.6379788E-1253.7037052
TAAACTA752.5345698E-548.3333365
TATTGTG450.00961483548.3333327
AGAGTGC450.00961483548.3333326
Produced by FastQC (version 0.11.5)