FastQCFastQC Report
Mon 4 Feb 2019
000000000-BTF83_l01_n02_Exp1_SrcID19_NL_Ind3_D3_rep2_SID34.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BTF83_l01_n02_Exp1_SrcID19_NL_Ind3_D3_rep2_SID34.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences194191
Sequences flagged as poor quality0
Sequence length151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTT26511.3651508051351504No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATTCACTATTTT14240.7332986595671273No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT12810.6596598194561025No Hit
AGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTCAATCC12750.6565700779129826No Hit
GTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTA8500.4377133852753217No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAA7820.402696314453296No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGTGAACTCTTCTTTCTTT7400.38106812365145654No Hit
TTCTTTATCCTTCCAGATTCGAAATCAATCCGTGCATCAATTCGGGCTCT6760.34811088052484407No Hit
GGATATTTGAAAAATTCTTCCCCAGCAGTTCATACAGAAGACCAGTCGGG6580.3388416558954843No Hit
ATACTGGATATCCCGACTGGTCTTCTGTATGAACTGCTGGGGAAGAATTT6410.3300873881899779No Hit
CCCTTATACTGGAGACCCTCCTTACAGCCATGGGACAGGAACAGGATATT6060.31206389585511174No Hit
GGACAAGCTAAATTCACTATTTTTGCCGTCTGAGCTCTTCAATGGTGGAA5680.29249553274868556No Hit
ATCCAGTATGGTGGAGGCTATGGTTTCCAGAGCCCGAATTGATGCACGGA5400.27807673888079265No Hit
GAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTT5300.27292716964225944No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGCTAAATTCACTATTTTTGCCG5290.2724122127184061No Hit
GCTAAATTCACTATTTTTGCCGTCTGAGCTCTTCAATGGTGGAACAGATC5200.2677776004037262No Hit
ATACTGGAGACCCTCCTTACAGCCATGGGACAGGAACAGGATATTTGAAA4570.2353353142009671No Hit
CTTCTTTCTTTATCCTTCCAGATTCGAAATCAATCCGTGCATCAATTCGG4450.22915583111472723No Hit
CCTTACAGCCATGGGACAGGAACAGGATATTTGAAAAATTCTTCCCCAGC4450.22915583111472723No Hit
GGATACACCATGGATACTGTCAACAGGACACATCAGTACTCAGAAAAGGG4200.21628190801839425No Hit
TAGTAGCACTGCCACAACTAACTCAATGCATGTGTAAGGAAGGAGTTGAA3920.2018631141505013No Hit
TCTTAAAAGTGCCAGCACAAAATGCTATAAGCACAACTTTCCCTTATACT3660.188474234130315No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG3610.1858994495110484No Hit
TCTTTATCCTTCCAGATTCGAAATCAATCCGTGCATCAATTCGGGCTCTG3470.17869005257710194No Hit
AGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTTT3360.1730255264147154No Hit
CCATTTGAATGGATGTCAATCCGACCTTACTTTTCTTAAAAGTGCCAGCA3310.1704507417954488No Hit
CTTAGGGACAACCTTGAACCTGGGACCTTTGATCTTGGGGGGCTATATGA3250.1673610002523289No Hit
GCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTTTC3200.16478621563306228No Hit
GTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATTCACTATTTTT3150.1622114310137957No Hit
GGATACACCATGGATACTGTCAACAGGACACATCAGTACTCAGAAAAGGA3150.1622114310137957No Hit
CCTCTTTGACTTGTATTCAAGATGGATCGATTTCTTTTGGGGATCCAGGA3120.16066656024223575No Hit
ATATGAAGCAATTGAGGAGTGCCTGATTAATGATCCCTGGGTTTTGCTTA3090.1591216894706758No Hit
GCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTC3070.15809177562296914No Hit
GTTCATACAGAAGACCAGTCGGGATATCCAGTATGGTGGAGGCTATGGTT2950.1519122925367293No Hit
GTATAAGGGAAAGTTGTGCTTATAGCATTTTGTGCTGGCACTTTTAAGAA2930.15088237868902266No Hit
CCCTTATACTGGAGACCCTCCTTACAGCCATGGGACAGGAACAGGATACA2900.1493375079174627No Hit
GTATGGTGGAGGCTATGGTTTCCAGAGCCCGAATTGATGCACGGATTGAT2870.14779263714590274No Hit
GTATGAACTGCTGGGGAAGAATTTTTCAAATATCCTGTTCCTGTCCCATG2700.13903836944039633No Hit
ATTTTTGCCGTCTGAGCTCTTCAATGGTGGAACAGATCTTCATGATCTCA2680.13800845559268968No Hit
ATGTCAATCCGACCTTACTTTTCTTAAAAGTGCCAGCACAAAATGCTATA2670.13749349866883637No Hit
AGCGAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTT2670.13749349866883637No Hit
CCACCATACTGGATATCCCGACTGGTCTTCTGTATGAACTGCTGGGGAAG2570.13234392943030315No Hit
AGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCAC2540.1307990586587432No Hit
TTCATGATCTCAGTGAACTCTTCTTTCTTTATCCTTCCAGATTCGAAATC2540.1307990586587432No Hit
ACCTTGAACCTGGGACCTTTGATCTTGGGGGGCTATATGAAGCAATTGAG2430.12513453249635667No Hit
AAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTTTCTTA2430.12513453249635667No Hit
AGCTAAATTCACTATTTTTGCCGTCTGAGCTCTTCAATGGTGGAACAGAT2390.1230747048009434No Hit
AAATCAATCCGTGCATCAATTCGGGCTCTGGAAACCATAGCCTCCACCAT2380.12255974787709009No Hit
GATCTTCATGATCTCAGTGAACTCTTCTTTCTTTATCCTTCCAGATTCGA2350.12101487710553013No Hit
GAGCGAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT2320.11947000633397017No Hit
GTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCACT2280.11741017863855689No Hit
AAGCACAACTTTCCCTTATACTGGAGACCCTCCTTACAGCCATGGGACAG2270.11689522171470357No Hit
GCCACAACTAACTCAATGCATGTGTAAGGAAGGAGTTGAACCAAGAAGCA2270.11689522171470357No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT2270.11689522171470357No Hit
GGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTTTCTTAAAAGTG2260.11638026479085024No Hit
GCTGTAAGGAGGGTCTCCAGTATAAGGGAAAGTTGTGCTTATAGCATTTT2260.11638026479085024No Hit
ATTGAGGAGTGCCTGATTAATGATCCCTGGGTTTTGCTTAATGCTTCTTG2260.11638026479085024No Hit
GATTTTCGGGGGGCTATATGAAGCAATTGAGGAGTGCCTGATTAATGATC2250.11586530786699693No Hit
GGATATCCCGACTGGTCTTCTGTATGAACTGCTGGGGAAGAATTTTTCAA2250.11586530786699693No Hit
CAGTGAACTCTTCTTTCTTTATCCTTCCAGATTCGAAATCAATCCGTGCA2220.11432043709543697No Hit
AGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTTT2210.11380548017158364No Hit
AAACAAGGGTGTTTTTTATTATTAAATAAGCTGAAACGAGAAAGTTCTTA2190.11277556632387702No Hit
GCAATAGCTGAATCAAGAAAACTGCTTCTTATCGTTCAGGCTCTTAGGGA2180.11226060940002368No Hit
GTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGATGTT2180.11226060940002368No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC2160.11123069555231706No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA2140.11020078170461041No Hit
GAGCAAAAGCAGGGTAGATAATCACTCACTGAGTGACATCAAAATCATGG2130.1096858247807571No Hit
GCTTCATATAGCCCCCCAAGATCAAAGGTCCCAGGTTCAAGGTTGTCCCT2130.1096858247807571No Hit
CCATACTGGATATCCCGACTGGTCTTCTGTATGAACTGCTGGGGAAGAAT2110.10865591093305045No Hit
GCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTAAGGAAGGAG2110.10865591093305045No Hit
AACTAACTCAATGCATGTGTAAGGAAGGAGTTGAACCAAGAAGCATTAAG2090.10762599708534382No Hit
TTTTTAAACTATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG2080.10711104016149049No Hit
CAAATATCCTGTTCCTGTCCCATGGCTGTAAGGAGGGTCTCCAGTATAAG2080.10711104016149049No Hit
GAATGGATGTCAATCCGACCTTACTTTTCTTAAAAGTGCCAGCACAAAAT2060.10608112631378386No Hit
AGTTCATACAGAAGACCAGTCGGGATATCCAGTATGGTGGAGGCTATGGT2050.10556616938993052No Hit
ATCCTGTTCCTGTCCCATGGCTGTAAGGAGGGTCTCCAGTATAAGGGAAA2040.10505121246607721No Hit
CCATTAGCCTTCTCTCCTTTCGCAAGGTTGCTCAGTTCATTGATGCTTAG2010.10350634169451725No Hit
CTATTTTTGCCGTCTGAGCTCTTCAATGGTGGAACAGATCTTCATGATCT2010.10350634169451725No Hit
CTCTAATATTGCTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAA2000.10299138477066394No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACGCG151.228491E-4145.0145
TTAGTAG151.228491E-4145.01
GCTCCGT100.0070864935145.0145
GAGCGAA1150.0138.695661
AACTGCG203.861315E-4108.75145
AGTAGAA3450.0107.173921
GAGCAAA7400.0105.810811
AGCAGGC4700.0103.351078
GCAAAAG8300.095.210843
AGGTAGT406.0987986E-790.6256
AAGCAGG10800.089.282417
CAAAAGC9050.087.320444
TATAGGC259.375277E-487.07
GCAGGCA5900.082.3305059
GTAGAAA4550.079.670332
AGAAACA4700.077.127664
AGCGAAA2250.074.111112
TACATGT300.001933385572.54
ACCAGCG300.001933385572.5145
GGTAGTG406.6496825E-572.52
Produced by FastQC (version 0.11.5)