FastQCFastQC Report
Mon 4 Feb 2019
000000000-BTF83_l01_n02_Exp1_SrcID19_NL_Ind3_D3_rep1_SID14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BTF83_l01_n02_Exp1_SrcID19_NL_Ind3_D3_rep1_SID14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences112769
Sequences flagged as poor quality0
Sequence length151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTT14931.3239454105294897No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT6080.5391552643013594No Hit
AGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTCAATCC5640.5001374491216558No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATTCACTATTTT4640.41146059644051114No Hit
GTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTA3560.3156895955448749No Hit
ATACTGGATATCCCGACTGGTCTTCTGTATGAACTGCTGGGGAAGAATTT3260.2890865397405315No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAA3170.2811056229992285No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGTGAACTCTTCTTTCTTT3040.2695776321506797No Hit
GGATATTTGAAAAATTCTTCCCCAGCAGTTCATACAGAAGACCAGTCGGG2990.26514378951662243No Hit
TTCTTTATCCTTCCAGATTCGAAATCAATCCGTGCATCAATTCGGGCTCT2580.2287862799173532No Hit
GAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTT2470.21903182612242728No Hit
CCCTTATACTGGAGACCCTCCTTACAGCCATGGGACAGGAACAGGATATT2260.20040968705938692No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGCTAAATTCACTATTTTTGCCG2190.19420230737170677No Hit
GGATACACCATGGATACTGTCAACAGGACACATCAGTACTCAGAAAAGGG2170.19242877031808386No Hit
CTTCTTTCTTTATCCTTCCAGATTCGAAATCAATCCGTGCATCAATTCGG2060.18267431652315796No Hit
ATCCAGTATGGTGGAGGCTATGGTTTCCAGAGCCCGAATTGATGCACGGA1980.17558016830866638No Hit
GGACAAGCTAAATTCACTATTTTTGCCGTCTGAGCTCTTCAATGGTGGAA1980.17558016830866638No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG1860.16493894598692901No Hit
GCTAAATTCACTATTTTTGCCGTCTGAGCTCTTCAATGGTGGAACAGATC1840.16316540893330614No Hit
CCTCTTTGACTTGTATTCAAGATGGATCGATTTCTTTTGGGGATCCAGGA1830.16227864040649467No Hit
GCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTTTC1760.15607126071881455No Hit
ATACTGGAGACCCTCCTTACAGCCATGGGACAGGAACAGGATATTTGAAA1740.15429772366519168No Hit
GGATACACCATGGATACTGTCAACAGGACACATCAGTACTCAGAAAAGGA1690.14986388103113443No Hit
CCTTACAGCCATGGGACAGGAACAGGATATTTGAAAAATTCTTCCCCAGC1650.14631680692388865No Hit
AGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTTT1620.1436565013434543No Hit
TAGTAGCACTGCCACAACTAACTCAATGCATGTGTAAGGAAGGAGTTGAA1610.14276973281664287No Hit
GCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTC1430.12680789933403686No Hit
GTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGATGTT1390.12326082522679106No Hit
GAGCAAAAGCAGGGTAGATAATCACTCACTGAGTGACATCAAAATCATGG1380.12237405669997961No Hit
CCATTAGCCTTCTCTCCTTTCGCAAGGTTGCTCAGTTCATTGATGCTTAG1310.11616667701229949No Hit
TCTTAAAAGTGCCAGCACAAAATGCTATAAGCACAACTTTCCCTTATACT1300.11527990848548804No Hit
GAGCAAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAA1290.1143931399586766No Hit
ATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTATGA1250.11084606585143081No Hit
CCACCATACTGGATATCCCGACTGGTCTTCTGTATGAACTGCTGGGGAAG1240.10995929732461937No Hit
CTTAGGGACAACCTTGAACCTGGGACCTTTGATCTTGGGGGGCTATATGA1230.10907252879780792No Hit
GTATGGTGGAGGCTATGGTTTCCAGAGCCCGAATTGATGCACGGATTGAT1220.10818576027099647No Hit
TCTTTATCCTTCCAGATTCGAAATCAATCCGTGCATCAATTCGGGCTCTG1220.10818576027099647No Hit
AGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTTT1210.10729899174418503No Hit
TTCATGATCTCAGTGAACTCTTCTTTCTTTATCCTTCCAGATTCGAAATC1190.10552545469056213No Hit
ATATGAAGCAATTGAGGAGTGCCTGATTAATGATCCCTGGGTTTTGCTTA1180.10463868616375069No Hit
CCATTTGAATGGATGTCAATCCGACCTTACTTTTCTTAAAAGTGCCAGCA1150.10197838058331635No Hit
GCAATAGCTGAATCAAGAAAACTGCTTCTTATCGTTCAGGCTCTTAGGGA1140.1010916120565049No Hit
CCATACTGGATATCCCGACTGGTCTTCTGTATGAACTGCTGGGGAAGAAT1140.1010916120565049No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTGGC100.00708258145.0145
GAGCGAA550.0118.636371
GAGCAAA5450.099.7706451
AGCAGGC3300.096.6666648
GCAAAAG5750.094.5652163
TAGTATA259.3650067E-487.01
GCAGGCA4000.083.3759
AAGCAGG7450.081.7449657
CAAAAGC6800.081.029414
AGTAGAA1800.072.51
AAAGCAG8550.071.228076
GTAGAAA1950.066.923082
AAAAGCA8700.063.3333325
AGTATAT350.00355830462.1428572
AGCGAAA1300.061.3461571
TACATCA606.766637E-660.4166684
CTATTCG753.7311474E-758.0000049
AGCAGGG1400.056.9642878
AGCAAAA9550.056.9371722
TAAACTA651.0873986E-555.7692345
Produced by FastQC (version 0.11.5)