FastQCFastQC Report
Mon 4 Feb 2019
000000000-BTF83_l01_n02_Exp1_SrcID18_NL_Ind2_D3_rep2_SID33.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BTF83_l01_n02_Exp1_SrcID18_NL_Ind2_D3_rep2_SID33.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences139247
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGTATCCTTCCAGATTCGAAATCAATCCGTGCATCAATTCGGGCTCTGG15011.0779406378593435No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTT12620.9063031878604207No Hit
CAGTGAACTCTTCTTTCTTTATCCTTCCAGATTCGAAATCAATCCGTGCA5680.40790824936982484No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT5500.39498157949542895No Hit
AGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTCAATCC5220.3748734263574799No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATTCACTATTTT5050.36266490480943936No Hit
GGATACACCATGGATACTGTCAACAGGACACATCAGTACTCAGAAAAGGG4340.3116763736382112No Hit
CCCTTATACTGGAGACCCTCCTTACAGCCATGGGACAGGAACAGGATACA3750.26930562238324707No Hit
GAACAAAGGTGCTGCAATTTATTTGAAAAATTCTTCCCCAGCAGTTCATA3480.24991561757165326No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG3390.24345228263445534No Hit
ATACTGGAGACCCTCCTTACAGCCATGGGACAGGAACAGGATACACCATG3370.24201598598174467No Hit
GTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTA3350.240579689329034No Hit
TCTCAGTGAACTCTTCTTTCTTTATCCTTCCAGATTCGAAATCAATCCGT3350.240579689329034No Hit
ATACTGGATATCCCGACTGGTCTTCTGTATGAACTGCTGGGGAAGAATTT3280.23555265104454673No Hit
CCTTACAGCCATGGGACAGGAACAGGATACACCATGGATACTGTCAACAG3040.21831709121201892No Hit
GAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTT3010.21616264623295295No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAA2970.21329005292753164No Hit
CTCAGTGAACTCTTCTTTCTTTATCCTTCCAGATTCGAAATCAATCCGTG2920.209699311295755No Hit
GAAGCAGTTTTCTTGATTCAGCTGAAAATCCTTCTAGTTGTGGAGATGCA2450.17594633995705475No Hit
GTCTGGCAGTGGCCCATCAATCGGGTTGAGTTGCGGTGCTCCAGTTTCGG2400.17235559832527808No Hit
GAGCAAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAA2340.16804670836714614No Hit
GTATGACCAAAGAGTTCTTTGAGAACAAATCAGAAACATGGCCCATTGGA2280.1637378184090142No Hit
TTTTTAAACTATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG2270.16301967008265889No Hit
CCTTTGTTCATCTTCAAGTACTCCTCTTTGACTTGTCTGGCAGTGGCCCA2200.1579926317981716No Hit
GGACAAGCTAAATTCACTATTTTTGCCGTCTGAGCTCTTCAATGGTGGAA2150.15440189016639497No Hit
CATCAGTACTCAGAAAAGGGAAGATGGACAACAAACACCGAAACTGGAGC2110.15152929686097366No Hit
GAGCAAAAGCAGGGTAGATAATCACTCACTGAGTGACATCAAAATCATGG2070.14865670355555236No Hit
GTACTCATGGTGGAGGCTATGGTTTCCAGAGCCCGAATTGATGCACGGAT2020.14506596192377574No Hit
CCATGGATACTGTCAACAGGACACATCAGTACTCAGAAAAGGGAAGATGG1940.13932077531293313No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA1860.13357558870209055No Hit
GTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACCACGTCTCCTTGC1830.13142114372302455No Hit
CACTTGGAAGTATGCTTCATGTATTCAGATTTTCACTTCATCAATGAGCA1800.12926669874395857No Hit
GTCCAGGCCATTGTGCGATCTCTTCCCTCGATTATTTCAAATCTGTGCTT1800.12926669874395857No Hit
GTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGATGTT1800.12926669874395857No Hit
CCTCTTTGACTTGTCTGGCAGTGGCCCATCAATCGGGTTGAGTTGCGGTG1800.12926669874395857No Hit
CCATACTGGATATCCCGACTGGTCTTCTGTATGAACTGCTGGGGAAGAAT1780.12783040209124794No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC1770.1271122537648926No Hit
GTACTGATGTGTCCTGTTGACAGTATCCATGGTGTATCCTGTTCCTGTCC1750.12567595711218194No Hit
GGATATCCCGACTGGTCTTCTGTATGAACTGCTGGGGAAGAATTTTTCAA1740.12495780878582663No Hit
CTCAGTATCCTTCCAGATTCGAAATCAATCCGTGCATCAATTCGGGCTCT1730.12423966045947131No Hit
GTACTCAGAAAAGGGAAGATGGACAACAAACACCGAAACTGGAGCACCGC1710.12280336380676064No Hit
GCTAAATTCACTATTTTTGCCGTCTGAGCTCTTCAATGGTGGAACAGATC1690.12136706715404999No Hit
ATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTATGA1660.11921262217498402No Hit
ATCCAGTATGGTGGAGGCTATGGTTTCCAGAGCCCGAATTGATGCACGGA1640.11777632552227336No Hit
CATCTTCAAGTACTCCTCTTTGACTTGTCTGGCAGTGGCCCATCAATCGG1560.11203113891143078No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGTATCCTTCCAGATTCGA1550.11131299058507545No Hit
GGATATTTGAAAAATTCTTCCCCAGCAGTTCATACAGAAGACCAGTCGGG1520.10915854560600946No Hit
GCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTTTC1510.10844039727965413No Hit
CATGTGGAGTTGATAATATGCACTCACTTGGAAGTATGCTTCATGTATTC1500.10772224895329882No Hit
ATTATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCA1500.10772224895329882No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT1460.1048496556478775No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGCTAAATTCACTATTTTTGCCG1450.10413150732152218No Hit
AAACAAGGGTGTTTTTTATTATTAAATAAGCTGAAACGAGAAAGTTCTTA1440.10341335899516688No Hit
CCTGTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGAT1420.10197706234245621No Hit
CCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATGTCAAAG1420.10197706234245621No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA450.0145.01
ATTGCAC100.007084353145.0145
AGCAGGC1950.0118.974368
GCAAAAG4650.0102.903233
GAGCAAA4800.099.68751
AAGCAGG6400.083.8281257
CAAAAGC5800.082.54
GCAGGCA3000.077.3333369
TACTTCA300.001932231572.5145
ATTCGAC607.980816E-872.51
TCGACAC607.980816E-872.53
ATATTTG851.6734703E-1068.235293
GCAGGGT1300.066.923079
AGTAGAA2000.065.251
AGCGAAA1350.064.444441
TAAACTA350.00356005962.1428575
CGACACT702.31983E-762.1428574
CTTGGGC350.00356005962.142857145
ACTAATT702.31983E-762.1428578
AAAGCAG8750.062.1428576
Produced by FastQC (version 0.11.5)