FastQCFastQC Report
Mon 4 Feb 2019
000000000-BTF83_l01_n02_Exp1_SrcID18_NL_Ind2_D3_rep1_SID13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BTF83_l01_n02_Exp1_SrcID18_NL_Ind2_D3_rep1_SID13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences119233
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGTATCCTTCCAGATTCGAAATCAATCCGTGCATCAATTCGGGCTCTGG13011.0911408754287821No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTT9510.7975979804248823No Hit
CAGTGAACTCTTCTTTCTTTATCCTTCCAGATTCGAAATCAATCCGTGCA4990.41850829887698876No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT3810.3195424085613882No Hit
AGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTCAATCC3020.2532855836890794No Hit
GAACAAAGGTGCTGCAATTTATTTGAAAAATTCTTCCCCAGCAGTTCATA2990.25076950173190304No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATTCACTATTTT2680.2247699881744148No Hit
CCCTTATACTGGAGACCCTCCTTACAGCCATGGGACAGGAACAGGATACA2500.2096734964313571No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG2450.20548002650272995No Hit
GGATACACCATGGATACTGTCAACAGGACACATCAGTACTCAGAAAAGGG2420.20296394454555367No Hit
CTCAGTGAACTCTTCTTTCTTTATCCTTCCAGATTCGAAATCAATCCGTG2230.18702875881677053No Hit
TCTCAGTGAACTCTTCTTTCTTTATCCTTCCAGATTCGAAATCAATCCGT2130.17864181895951625No Hit
CCTTACAGCCATGGGACAGGAACAGGATACACCATGGATACTGTCAACAG2020.16941618511653653No Hit
GAGCAAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAA2010.16857749113081108No Hit
GAAGCAGTTTTCTTGATTCAGCTGAAAATCCTTCTAGTTGTGGAGATGCA2010.16857749113081108No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAA1950.16354532721645854No Hit
ATACTGGATATCCCGACTGGTCTTCTGTATGAACTGCTGGGGAAGAATTT1870.1568357753306551No Hit
ATACTGGAGACCCTCCTTACAGCCATGGGACAGGAACAGGATACACCATG1820.15264230540202797No Hit
GTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTA1760.1476101414876754No Hit
GAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTT1690.14173928358759738No Hit
GTATGACCAAAGAGTTCTTTGAGAACAAATCAGAAACATGGCCCATTGGA1680.14090058960187196No Hit
GTACTCATGGTGGAGGCTATGGTTTCCAGAGCCCGAATTGATGCACGGAT1530.12832017981599053No Hit
GAGCAAAAGCAGGGTAGATAATCACTCACTGAGTGACATCAAAATCATGG1480.1241267098873634No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA1430.11993323995873625No Hit
CCTTTGTTCATCTTCAAGTACTCCTCTTTGACTTGTCTGGCAGTGGCCCA1390.11657846401583453No Hit
GTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACCACGTCTCCTTGC1360.11406238205865825No Hit
ATCCAGTATGGTGGAGGCTATGGTTTCCAGAGCCCGAATTGATGCACGGA1280.10735283017285482No Hit
CCATGGATACTGTCAACAGGACACATCAGTACTCAGAAAAGGGAAGATGG1270.10651413618712939No Hit
GTCCAGGCCATTGTGCGATCTCTTCCCTCGATTATTTCAAATCTGTGCTT1260.10567544220140399No Hit
CACTTGGAAGTATGCTTCATGTATTCAGATTTTCACTTCATCAATGAGCA1250.10483674821567855No Hit
GCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTTTC1230.1031593602442277No Hit
CCTGTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGAT1230.1031593602442277No Hit
GTCTGGCAGTGGCCCATCAATCGGGTTGAGTTGCGGTGCTCCAGTTTCGG1220.10232066625850227No Hit
CATCTTCAAGTACTCCTCTTTGACTTGTCTGGCAGTGGCCCATCAATCGG1220.10232066625850227No Hit
GGATATCCCGACTGGTCTTCTGTATGAACTGCTGGGGAAGAATTTTTCAA1210.10148197227277683No Hit
GTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGATGTT1210.10148197227277683No Hit
GGACAAGCTAAATTCACTATTTTTGCCGTCTGAGCTCTTCAATGGTGGAA1200.10064327828705141No Hit
CTCAGTATCCTTCCAGATTCGAAATCAATCCGTGCATCAATTCGGGCTCT1200.10064327828705141No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAGCG100.0070830854145.0145
GAGCGAA600.0120.8333361
GAGCAAA4450.0102.640461
AGCAGGC2100.0100.119058
GCAAAAG5150.091.504853
CAAAAGC5500.085.6818164
GCAGGAG353.424844E-582.857149
AAGCAGG6650.079.5864647
GCAGGCA2650.079.339629
AAAGCAG7300.073.493166
TTTCGCT406.639158E-572.53
TTCGCTC300.001931547772.54
GCAGGGT1900.068.684219
AGCAAAA6900.067.2463762
TAGTAAA808.005372E-963.43752
AGCAGGG2300.063.0434728
AAAAGCA7700.061.20135
CGAAAGC1200.060.4166683
TCGTACT606.769007E-660.4166682
GGATAGC753.7329664E-758.05
Produced by FastQC (version 0.11.5)