FastQCFastQC Report
Mon 4 Feb 2019
000000000-BTF83_l01_n02_Exp1_SrcID17_NL_Ind1_D3_rep2_SID32.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BTF83_l01_n02_Exp1_SrcID17_NL_Ind1_D3_rep2_SID32.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences162301
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTT14080.8675239216024546No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT12310.7584672922532825No Hit
AGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTCAATCC11080.6826821769428407No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAA7410.4565591093092464No Hit
GTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTA6990.4306812650569004No Hit
GAGCAAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAA5480.33764425357822814No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATTCACTATTTT5220.32162463570772826No Hit
CCCTTATACTGGAGACCCTCCTTACAGCCATGGGACAGGAACAGGATACA4290.2643236948632479No Hit
TTTTTAAACTATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG4270.26309141656551716No Hit
GGATACACCATGGATACTGTCAACAGGACACATCAGTACTCAGAAAAGGG3390.20887117146536374No Hit
TAGTAGCACTGCCACAACTAACTCAATGCATGTGTAAGGAAGGAGTTGAA3360.20702275401876757No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGTGAACTCTTCTTTCTTT3310.2039420582744407No Hit
GGAGAATACAAGTCAAAGAGGAGTACTTGAAGATGAACAAATGTACCAAA3310.2039420582744407No Hit
GTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACCACGTCTCCTTGC3250.20024522338124842No Hit
ATTATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCA3060.1885385795528062No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG2980.18360946636188316No Hit
GTATTCTCCAGTTTCGGTGTTTGTTGTCCATCTTCCCTTTTCTGAGTACT2900.17868035317096012No Hit
GCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTC2810.1731351008311717No Hit
ATATGAAGCAATTGAGGAGTGCCTGATTAATGATCCCTGGGTTTTGCTTA2710.16697370934251793No Hit
ACCTTGAACCTGGGACCTTTGATCTTGGGGGGCTATATGAAGCAATTGAG2710.16697370934251793No Hit
ATACTGGATATCCCGACTGGTCTTCTGTATGAACTGCTGGGGAAGAATTT2600.16019617870499872No Hit
TTCTTTATCCTTCCAGATTCGAAATCAATCCGTGCATCAATTCGGGCTCT2460.15157023062088343No Hit
ATACTGGAGACCCTCCTTACAGCCATGGGACAGGAACAGGATACACCATG2440.15033795232315267No Hit
AGCGAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTT2410.14848953487655653No Hit
GAAGCAGTTTTCTTGATTCAGCTGAAAATCCTTCTAGTTGTGGAGATGCA2370.146024978281095No Hit
GGACAAGCTAAATTCACTATTTTTGCCGTCTGAGCTCTTCAATGGTGGAA2350.14479269998336425No Hit
CTTCTTTCTTTATCCTTCCAGATTCGAAATCAATCCGTGCATCAATTCGG2330.1435604216856335No Hit
GAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTT2330.1435604216856335No Hit
CCTTACAGCCATGGGACAGGAACAGGATACACCATGGATACTGTCAACAG2310.14232814338790273No Hit
GCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTTTC2250.13863130849471045No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT2170.1337021953037874No Hit
GAGCGAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT2140.13185377785719127No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA2010.12384396892194133No Hit
AGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCAC2010.12384396892194133No Hit
GCTGGGGAAGAATTTTTCAAATAAATTGCAGCACCTTTGGTACATTTGTT2000.12322782977307596No Hit
GTGTAAGGAAGGAGTTGAACCAAGAAGCATTAAGCAAAACCCAGGGATCA1960.12076327317761444No Hit
ATCCAGTATGGTGGAGGCTATGGTTTCCAGAGCCCGAATTGATGCACGGA1940.11953099487988367No Hit
GCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTAAGGAAGGAG1940.11953099487988367No Hit
AGCGAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAAG1920.11829871658215292No Hit
GTATGGGGAGGACCTGAAAATCGAAACAAACAAATTTGCAGCAATATGCA1910.11768257743328754No Hit
GCCACAACTAACTCAATGCATGTGTAAGGAAGGAGTTGAACCAAGAAGCA1900.11706643828442215No Hit
CTTCCAAGTGAGTGCATATTGCTGCAAATTTGTTTGTTTCGATTTTCAGG1900.11706643828442215No Hit
GTAGAAACAAGGTCGTTTTTAAACTATTCGACACTAATTGATGGCCATCC1830.1127534642423645No Hit
CTCTTCTTTCTTTATCCTTCCAGATTCGAAATCAATCCGTGCATCAATTC1810.11152118594463373No Hit
GTATTCAAGATGGATCGATTTCTTTTGGGGATCCAGGAGTGTGTTGTTGC1800.11090504679576836No Hit
CTTAGGGACAACCTTGAACCTGGGACCTTTGATCTTGGGGGGCTATATGA1800.11090504679576836No Hit
GAGCAAAAGCAGGGTAGATAATCACTCACTGAGTGACATCAAAATCATGG1790.11028890764690298No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGCTAAATTCACTATTTTTGCCG1780.1096727684980376No Hit
TCATTAATCAGGCACTCCTCAATTGCTTCATATAGCCCCCCAAGATCAAA1780.1096727684980376No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC1750.10782435105144146No Hit
ACCTTGGACCTTTGATCTTGGGGGGCTATATGAAGCAATTGAGGAGTGCC1710.10535979445597994No Hit
GTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGATGTT1710.10535979445597994No Hit
AACTAACTCAATGCATGTGTAAGGAAGGAGTTGAACCAAGAAGCATTAAG1710.10535979445597994No Hit
GTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCACT1680.10351137700938379No Hit
GTACTGATGTGTCCTGTTGACAGTATCCATGGTGTATCCTGTTCCTGTCC1670.10289523786051842No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT1670.10289523786051842No Hit
GCTTCATATAGCCCCCCAAGATCAAAGGTCCCAGGTTCAAGGTTGTCCCT1650.10166295956278767No Hit
TCAATGAACTGAGCAACCTTGCGAAAGGAGAGAAGGCTAATGTGCTAATT1640.10104682041392227No Hit
GATCTTCATGATCTCAGTGAACTCTTCTTTCTTTATCCTTCCAGATTCGA1640.10104682041392227No Hit
GCATACTTCCAAGTGAGTGCATATTGCTGCAAATTTGTTTGTTTCGATTT1630.1004306812650569No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTCTAC100.0070854286145.0145
GAGCGAA1200.0138.958331
TCGTACT256.448441E-6116.02
GGCAGGT203.86016E-4108.757
GAGCAAA6700.0107.126861
AGCAGGC2800.098.392868
GCAAAAG8250.087.878793
CGGCCGG259.372485E-487.0145
TACGGCA259.372485E-487.04
TTATATA259.372485E-487.04
TAGGCAT259.372485E-487.09
CAAAAGC8400.086.3095254
GCAGGCA3250.084.769239
GTCGTAC353.42859E-582.857141
AAGCAGG10850.082.188947
AAGGGTG451.2282198E-680.555565
ATAGCGG300.001932811872.53
AGCGGTA300.001932811872.55
ACGGCAG300.001932811872.55
GCAGGAG502.2975346E-672.59
Produced by FastQC (version 0.11.5)