FastQCFastQC Report
Mon 4 Feb 2019
000000000-BTF83_l01_n02_Exp1_SrcID17_NL_Ind1_D3_rep1_SID12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BTF83_l01_n02_Exp1_SrcID17_NL_Ind1_D3_rep1_SID12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66920
Sequences flagged as poor quality0
Sequence length151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTT4670.6978481769276748No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT3390.5065750149432158No Hit
AGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTCAATCC2620.39151225343693963No Hit
GTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTA1930.28840406455469214No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAA1450.21667662881052005No Hit
GAGCAAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAA1420.21219366407650925No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATTCACTATTTT1370.20472205618649134No Hit
CCCTTATACTGGAGACCCTCCTTACAGCCATGGGACAGGAACAGGATACA1040.15540944411237298No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG1010.1509264793783622No Hit
GGAGAATACAAGTCAAAGAGGAGTACTTGAAGATGAACAAATGTACCAAA1000.14943215780035865No Hit
GTATTCTCCAGTTTCGGTGTTTGTTGTCCATCTTCCCTTTTCTGAGTACT1000.14943215780035865No Hit
GGATACACCATGGATACTGTCAACAGGACACATCAGTACTCAGAAAAGGG1000.14943215780035865No Hit
GTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACCACGTCTCCTTGC870.13000597728631202No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGTGAACTCTTCTTTCTTT800.11954572624028689No Hit
ACCTTGAACCTGGGACCTTTGATCTTGGGGGGCTATATGAAGCAATTGAG790.11805140466228334No Hit
TAGTAGCACTGCCACAACTAACTCAATGCATGTGTAAGGAAGGAGTTGAA790.11805140466228334No Hit
GCTGGGGAAGAATTTTTCAAATAAATTGCAGCACCTTTGGTACATTTGTT780.11655708308427974No Hit
ATTATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCA760.11356843992827256No Hit
AGCGAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTT760.11356843992827256No Hit
TTTTTAAACTATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG750.11207411835026898No Hit
ATACTGGAGACCCTCCTTACAGCCATGGGACAGGAACAGGATACACCATG750.11207411835026898No Hit
CTCTTCTTTCTTTATCCTTCCAGATTCGAAATCAATCCGTGCATCAATTC740.11057979677226538No Hit
GCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTC730.1090854751942618No Hit
CTTCTTTCTTTATCCTTCCAGATTCGAAATCAATCCGTGCATCAATTCGG720.10759115361625822No Hit
CCTTACAGCCATGGGACAGGAACAGGATACACCATGGATACTGTCAACAG720.10759115361625822No Hit
CTTCCAAGTGAGTGCATATTGCTGCAAATTTGTTTGTTTCGATTTTCAGG710.10609683203825462No Hit
GAAGCAGTTTTCTTGATTCAGCTGAAAATCCTTCTAGTTGTGGAGATGCA690.10310818888224747No Hit
GAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTT690.10310818888224747No Hit
ATACTGGATATCCCGACTGGTCTTCTGTATGAACTGCTGGGGAAGAATTT670.1001195457262403No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCAAA3000.0118.416671
TATATAG256.4205324E-6116.05
TATAGGT352.7334318E-7103.571437
GCAAAAG3400.0100.220593
AGCAGGC1800.096.6666648
GAGCGAA554.0745363E-1092.2727361
AAGCAGG4200.091.488097
CAAAAGC3650.091.3698654
CTACTAG259.3482324E-487.01
TAGACAG259.3482324E-487.05
GCAGGCA2000.087.09
GCAGGTC950.083.947379
GTATATA353.4138375E-582.857144
AAAGCAG4850.080.721656
AAAAGCA4300.079.244195
AGCAGGT2200.072.500018
CTATATA300.001927828672.52
TAGGTCC502.282768E-672.59
ACGATCC300.001927828672.5145
ATATAGG406.617879E-572.56
Produced by FastQC (version 0.11.5)