FastQCFastQC Report
Mon 4 Feb 2019
000000000-BTF83_l01_n02_Exp1_SrcID12_Shed_Cnt2_D3_rep2_SID31.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BTF83_l01_n02_Exp1_SrcID12_Shed_Cnt2_D3_rep2_SID31.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60407
Sequences flagged as poor quality0
Sequence length151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATGTCAAAG670.11091429801181982No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGTAGT100.007074494145.06
GTAGTTT100.007074494145.08
GGTAGTT100.007074494145.07
CAAGGGT256.415432E-6116.04
GCAGGTC203.8483014E-4108.758
GCAGGGT451.218863E-680.555568
CGAAAGC651.5315891E-978.076923
TGTTAAG300.001926917872.52
CTATTAG300.001926917872.54
AGCGAAA754.7530193E-967.6666641
AAGGGTG451.1848329E-464.444445
AGGGTGT501.9950909E-458.06
TAGAGCC400.006023505254.3758
TTAGAGC400.006023505254.3757
CAGGGTA400.006023505254.3759
GTTCATG400.006023505254.3757
GAAAGCA953.0739102E-853.4210554
AGCAGGT701.6769236E-551.7857137
AGCAGGG701.6769236E-551.7857137
AAGCAGG1550.051.4516146
Produced by FastQC (version 0.11.5)