FastQCFastQC Report
Mon 4 Feb 2019
000000000-BTF83_l01_n02_Exp1_SrcID12_Shed_Cnt2_D3_rep1_SID11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BTF83_l01_n02_Exp1_SrcID12_Shed_Cnt2_D3_rep1_SID11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79080
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTCAATCC1750.22129489124936771No Hit
GTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTA1420.17956499747091553No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT1370.17324228629236216No Hit
GTATACATCATGGTCATAAGTCCCATTTCTGATTGACTCTATGCAAGCGT1210.1530096105209914No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAA1120.14162873039959534No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT1110.14036418816388468No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA1010.12771876580677793No Hit
AAACAAGGGTGTTTTTTATTATTAAATAAGCTGAAACGAGAAAGTTCTTA990.1251896813353566No Hit
GTGTTATCATTCCATTCAAGTCCCCCGATGAGGACTCCAACTGCATTTTT960.12139605462822459No Hit
CTCTAATATTGCTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAA950.12013151239251392No Hit
ATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTATGA920.11633788568538189No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGGCGT100.007078602145.04
GCAGGAG203.8527552E-4108.757
GAGCGAA259.354571E-487.01
GATGTAC300.001929130972.56
GTACACC300.001929130972.59
TACATGA300.001929130972.52
TATACTC406.6253284E-572.55
GCAGGGT554.0271334E-665.9090969
AGCTTAT451.1870945E-464.444441
CTTATAT350.003554368662.1428533
GTACTAC350.003554368662.1428533
TCATATC350.003554368662.1428533
TGTACTA350.003554368662.1428532
TACTGAT501.9988888E-458.07
GTATACT501.9988888E-458.04
GTAGAAA902.0174411E-856.388892
TATATAC400.006030373354.3753
CTAGTAG400.006030373354.3753
GTACTGA805.80445E-754.3756
TATAGGC400.006030373354.3757
Produced by FastQC (version 0.11.5)