FastQCFastQC Report
Mon 4 Feb 2019
000000000-BTF83_l01_n02_Exp1_SrcID10_Shed_Ind3_D3_rep2_SID30.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BTF83_l01_n02_Exp1_SrcID10_Shed_Ind3_D3_rep2_SID30.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences142094
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTT14000.9852632764226498No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT6870.48348276493025744No Hit
AGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTCAATCC6660.4687038157839177No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATTCACTATTTT6050.4257744873112165No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAA4500.31669176742156596No Hit
GTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTA4320.3040240967247034No Hit
GGATATTTGAAAAATTCTTCCCCAGCAGTTCATACAGAAGACCAGTCGGG4190.2948752234436359No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGTGAACTCTTCTTTCTTT3450.24279702168986728No Hit
ATACTGGATATCCCGACTGGTCTTCTGTATGAACTGCTGGGGAAGAATTT3170.22309175616141425No Hit
TTCTTTATCCTTCCAGATTCGAAATCAATCCGTGCATCAATTCGGGCTCT3070.2160541613298239No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG2970.20901656649823355No Hit
GAGCAAAAGCAGGGTAGATAATCACTCACTGAGTGACATCAAAATCATGG2790.19634889580137094No Hit
GAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTT2750.19353385786873478No Hit
GGATACACCATGGATACTGTCAACAGGACACATCAGTACTCAGAAAAGGG2600.18297746562134926No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA2590.18227370613819022No Hit
CCCTTATACTGGAGACCCTCCTTACAGCCATGGGACAGGAACAGGATACA2440.17171731389080466No Hit
GGACAAGCTAAATTCACTATTTTTGCCGTCTGAGCTCTTCAATGGTGGAA2420.1703097949244866No Hit
GGATACACCATGGATACTGTCAACAGGACACATCAGTACTCAGAAAAGGA2420.1703097949244866No Hit
GTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGATGTT2350.16538347854237334No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGCTAAATTCACTATTTTTGCCG2100.14778949146339745No Hit
CCTCTTTGACTTGTATTCAAGATGGATCGATTTCTTTTGGGGATCCAGGA2050.1442706940476023No Hit
CCTGTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGAT1990.14004813714864808No Hit
GCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTTTC1980.13934437766548904No Hit
CTTCTTTCTTTATCCTTCCAGATTCGAAATCAATCCGTGCATCAATTCGG1960.13793685869917097No Hit
CCATTAGCCTTCTCTCCTTTCGCAAGGTTGCTCAGTTCATTGATGCTTAG1940.13652933973285292No Hit
GAGCAAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAA1900.13371430180021676No Hit
TAGTAGCACTGCCACAACTAACTCAATGCATGTGTAAGGAAGGAGTTGAA1890.13301054231705772No Hit
ATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTATGA1870.13160302335073965No Hit
GCTAAATTCACTATTTTTGCCGTCTGAGCTCTTCAATGGTGGAACAGATC1840.12949174490126256No Hit
ATACTGGAGACCCTCCTTACAGCCATGGGACAGGAACAGGATACACCATG1840.12949174490126256No Hit
TTTTTAAACTATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG1800.1266767069686264No Hit
ATCCAGTATGGTGGAGGCTATGGTTTCCAGAGCCCGAATTGATGCACGGA1730.12175039058651316No Hit
AGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTTT1670.11752783368755894No Hit
GCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTC1630.1147127957549228No Hit
TCTTAAAAGTGCCAGCACAAAATGCTATAAGCACAACTTTCCCTTATACT1600.1126015173054457No Hit
CCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATGTCAAAG1580.11119399833912762No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA1560.10978647937280954No Hit
CCCTTATACTGGAGACCCTCCTTACAGCCATGGGACAGGAACAGGATATT1510.10626768195701437No Hit
CTCTAATATTGCTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAA1510.10626768195701437No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC1500.10556392247385535No Hit
CTTAGGGACAACCTTGAACCTGGGACCTTTGATCTTGGGGGGCTATATGA1500.10556392247385535No Hit
CAAATATCCTGTTCCTGTCCCATGGCTGTAAGGAGGGTCTCCAGTATAAG1480.10415640350753726No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT1470.10345264402437822No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT1450.10204512505806015No Hit
GTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATTCACTATTTTT1440.10134136557490112No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGGTC100.0070845042145.00002145
AACTGCG100.0070845042145.00002145
TCTAGGG151.2278021E-4145.0145
TTATATA256.445647E-6116.04
TTAGTAG256.445647E-6116.01
GAGCGAA850.0110.882351
GAGCAAA4800.0110.2604141
CGAATCG203.859157E-4108.75001145
AGCAGGC2450.0100.612248
GCAAAAG5500.093.590913
TCGTACT353.427112E-582.857152
CAAAAGC6550.078.587784
GCAGGCA3250.075.8461469
AAGCAGG8000.075.218757
CAAGGTC406.64354E-572.500018
TATAGGC406.64354E-572.500017
CTATGTA300.001932313272.53
CGGGCTT300.001932313272.5145
GTCGTCA300.001932313272.59
AGTCGTC300.001932313272.58
Produced by FastQC (version 0.11.5)