FastQCFastQC Report
Mon 4 Feb 2019
000000000-BTF83_l01_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BTF83_l01_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1576809
Sequences flagged as poor quality0
Sequence length151
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1095816.949541764411543No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGC16860.10692480826783714No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGCG16480.10451487783238174No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGA15870.10064630529125596No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT15850.10051946684728462No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCAAA14300.072.51
GCAAAAG16150.065.54183
CTCCGAC2850.043.245613145
AGCAGGC7350.042.4149678
CTATTCG2400.042.2916689
AGCAAAA25350.041.469432
TGTTACG700.001056826941.42857145
TACGCAT1252.7202805E-740.6145
CTCGTAG1209.567353E-636.25145
CTTATAT1001.3918137E-436.253
CGAAAGC5800.035.03
CAGTATC5000.034.81
GCAGGTA11450.034.1921429
AAAGCAG35350.034.045266
CAAAAGC32800.032.271344
GCAGGTC5400.032.222229
GAGCGAA4300.032.0348851
TAAACTA2950.031.9491545
GCAGGCA10300.029.5631089
TTAACTC1000.006075506629.0145
Produced by FastQC (version 0.11.5)