FastQCFastQC Report
Mon 4 Feb 2019
000000000-BTF83_l01_n01_Exp3_Neg_control_rep2_SID92.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BTF83_l01_n01_Exp3_Neg_control_rep2_SID92.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGGAAGCACATATCCAGTGCTGAACGTGACTATGCCAAACAATGACAAT12.941176470588235No Hit
CCTCAATTGCTTCATATAGCCCCCCAAGATCAAAGGCCCCAGGTTCAAGG12.941176470588235No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGCGGGATAGGGG12.941176470588235No Hit
ACCTACATCTTTACAGTCGTCAAAGTCTACCTTTTCTGGTGCAATGTTCT12.941176470588235No Hit
ACTCAATGCATGTGTAAGGAAGGAGTTGAACCAAGAAGCATTAAGCAAAA12.941176470588235No Hit
GTATGGGGAGGACCTGAAAATCGAAACAAACAAATTTGCAGCAGAAGGAT12.941176470588235No Hit
CCATAAGACAAGAAATGGCCAGCAGAGGCCTCTGGGATTCCTTTCGTCAG12.941176470588235No Hit
CCTCATTCTGGGCAAAGAAGACAAGAGATATGGGCCAGCACTAAGCATCA12.941176470588235No Hit
GGTTCAAGGTTGTCCCTAAGAGCCTGAACGATAAGAAGCAGTTTTCTTGA12.941176470588235No Hit
CTCTTAGATCTTCAAATGCGGCAGAATGGCATGCCATCCACACCAGTTGA12.941176470588235No Hit
GTCCTGCAGTTCTGCCAGTACTTGCTTCCATGACAGAAGATAATTTGGAT12.941176470588235No Hit
GTAAAATGCTATAAGCACAACTTTCCTTTATACTGGAGACCTTCCTTACA12.941176470588235No Hit
GATGTAAGGCTTGCATAAATGTTATTTGCTCAAAACTATTCTCTGTTATC12.941176470588235No Hit
GCAATATGCACTCACTTGGAAGTATGCTTCATGTATTCAGATTTTCACTT12.941176470588235No Hit
TCTCTCTATTGTTAAGCTGTTTTGGATCAACCGTCCCTCATAATCACTGA12.941176470588235No Hit
TCTTAGGGATTTCTGATCTCGGCGAAGCCGATCAAGGAATGGGGCATCAC12.941176470588235No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGT12.941176470588235No Hit
CTTGGAGAAGCAGTAATGAGAATGGGAGACCTCCACTCACTCCAAAACAG12.941176470588235No Hit
ACTCAGTGCGAAAGCATACATTGGTGCTATTAGATTTCCATTTGCCTCAA12.941176470588235No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAA12.941176470588235No Hit
GTAAGCTACTTGGAATCAATATGAGCAAGAAAAAGTCTTACATAAACAGA12.941176470588235No Hit
CACAAAAACCACCGTGGACCATATGGCCATAATCAAGAAGTACACATCAG12.941176470588235No Hit
GCTTGTATGCATCTCCACAACTAGAAGGATTTTCAGCTGAATCAAGAAAA12.941176470588235No Hit
CTTAAAATGACCATGGCCTCTGTACCTGCGTCGCGTTACCTAACTGACAT12.941176470588235No Hit
TTGCTCATTGATGAAGTGAAAATCTGAATACATGAAGCATACTTCCAAGT12.941176470588235No Hit
ATTTTTGCCGTCTGAGCTCTTCAATGGTGGAACAGATCTTCTTCTTTCTT12.941176470588235No Hit
GTAAAGATGTAGGTGATTTGAAGCAATATGATAGTGATGAACCAGAATTG12.941176470588235No Hit
GTAGTAAACAGTATTTGCAACACTACAGGGGCTGAGAAACCAAAGTTTCT12.941176470588235No Hit
CTCCAACATGTATGCAACCATCAAAGGAGAAATTTTGCAATCCTGGAGTT12.941176470588235No Hit
GGCTAATTGGGCAAGGAGACGTGGTGTTGGTAATGAAACGGAAACGGGAC12.941176470588235No Hit
GCCTATGAGACCGATGCTGGGAGTCAGCAATCTGTTCACAGGTTGCACAT12.941176470588235No Hit
ATTGGGGGACCCTCATTGTGATGTTTTTCAAAATGAGACATGGGACCTTT12.941176470588235No Hit
GTCGTAGACATAAATATGGAAGATTATAGCATTGATTCCAGTTATGTGTG12.941176470588235No Hit
TTCTTTATCCTTCCAGATTCGAAATCAATCCGTGCATCAATTCGGGCTCT12.941176470588235No Hit

[WARN]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)