FastQCFastQC Report
Mon 4 Feb 2019
000000000-BTF83_l01_n01_Exp3_Neg_control_rep1_SID90.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BTF83_l01_n01_Exp3_Neg_control_rep1_SID90.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTT33.8461538461538463No Hit
TAGTAGCACTGCCACAACTAACTCAATGCATGTGTAAGGAAGGAGTTGAA22.564102564102564No Hit
ATGGTGGAGGCTATGGTTTCCAGAGCCCGAATTGATGCACGGATTGATTT11.282051282051282No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGCGGGGTGCGGGGGTTG11.282051282051282No Hit
GAGCTAGGATGAGTCCCAATGGTTCTCATCGCTTGCACCATTTGTCTAGC11.282051282051282No Hit
GTAGAATGCAGTTCTCCTCATTTACTGTGAATGTGAGGGGATCAGGAATG11.282051282051282No Hit
GGTTTTAGCCAGCACTACAGCTAAGGCTATGGAGCAAATGGCTGGATCGA11.282051282051282No Hit
GAACTGAGCAACCTTGCGAAAGGAGAGAAGGCTAATGTGCTAATTGGGCA11.282051282051282No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAGAGAATCTCGTATGCCGTC11.282051282051282No Hit
GGGTGTTTTTAATTACTAATACACTCAAATGCAAATGTTGCACCTAATGT11.282051282051282No Hit
TCCTGTGATTTCAAACCTTTCTTCAATTGTCTCTTCTCCTCTCTCGGACT11.282051282051282No Hit
GCCATGCTTTTCCATCGTGACAACTTGAGCTGGACCATGCTATACACACT11.282051282051282No Hit
GGATACTGTCAACAGGACACATCAGTACTCAGAAAAGGGAAGATGGACAA11.282051282051282No Hit
GCAGAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAACAAAAAAAATA11.282051282051282TruSeq Adapter, Index 25 (96% over 27bp)
GCATTAAACAACCGGTTCCAGATCAAAGGCGTTGAGCTGAAGTCAGGATA11.282051282051282No Hit
TTCACCGAAGAGGGAGCAATTGTTGGCGAAATTTCACCATTGCCTTCTCT11.282051282051282No Hit
CCTATTGATGCAGCTTTTGCCCTCAACAGAGAAAATACCAGAGTAACCTG11.282051282051282No Hit
GGATATTTGAAAAATTCTTCCCCAGCAGTTCATACAGAAGACCAGTCGGG11.282051282051282No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGTGCGGGCGGGGGGGAGGAGGCGGG11.282051282051282No Hit
GTACCAGAGAAACAAACTAGAGGCCTATTCGGCGCAATAGCAGGTTTCAT11.282051282051282No Hit
GTGGTTATGCCCAAACAGATTGTGTATTGGAAGCAATGGCTTTCCTTGAG11.282051282051282No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11.282051282051282No Hit
ACTTTCCCTTATACTGGAGACCCTCCTTACAGCCATGGGACAGGAACAGG11.282051282051282No Hit
GCTATAAGCACAACTTTCCCTTATACTGGAGACCCTCCTTACATCCATGG11.282051282051282No Hit
TGGTTTTAGCCAGCACTACAGCTAAGGCTATGGAGCAAATGGCTGGATCG11.282051282051282No Hit
GTACTGAGAACGTAGGTTGTATGCTGATTTGGCCCGCAGATGCCCTCTGT11.282051282051282No Hit
AGTACGTCTCCCGGCTTAACTATTGTCCAATAGATGCTTATTCTACTAGA11.282051282051282No Hit
CCATAGCCTCCACCATACTGGATATCCCGACTGGTATCCATGGTGTATCC11.282051282051282No Hit
GAACTTCTGGAGGGGTGAGAATGGACGAAAAACAAGAATTGCTTATGAAA11.282051282051282No Hit
TTAGTAGATTGAATTGGTTGACCCACTTAAAATTCAAATACCCAGCATTG11.282051282051282No Hit
GGGAAAAGCTCAATAATGAGGTCAGATGCACCTATTGATACCTGTATTTC11.282051282051282No Hit
ACTGTCAACAGGACACATCAGTACTCAGAAAAGGGAAGATGGACAACAAA11.282051282051282No Hit
GATCAGTGTTCTTCCCTGCAAAGACATCTTCAAGTCTCTGTGCGATCTCG11.282051282051282No Hit
GGTCATAAGTCCCATTTCTGATTGACTCTATGCAAGCGTTGTCACATTTG11.282051282051282No Hit
ATTGAGGGCAAGCTGTCTCAAATGTCCAAAGAAGTAAATGCTAGAATTGA11.282051282051282No Hit
GGAAGAGTCACAGTCTCTACCAGGAGAAGCCAGCAAACTATAATCCCGAA11.282051282051282No Hit
GGGTAGATAATCACTCACTGAGTGACATCAAAATCATGGCGTCCCAAGGC11.282051282051282No Hit
TGGCTGGATCGAGTGAGCAAGCAGCAGAGGCCATGGAGGTTGCTAGTCAG11.282051282051282No Hit
GCACTCTGCTGTTCCTTTCGATATTCTTCCCTCATAGACTTTGGCACTCC11.282051282051282No Hit
GACCAGCACTGGAGCTAGGATGAGTCCCAATGGTTCTCATCGCTTGCACC11.282051282051282No Hit
CCTGGATCCCCAAAGGAAATCGATCCATCTTGAATACAAGTCAAAGAGGA11.282051282051282No Hit
CATGGAGGTTGCTAGTCAGGCTAGACAAATGGTGCAAGCGATGAGAACCA11.282051282051282No Hit
CCATAGCCTCCACCAGACTGGATATCCCGACTGGTCTTCTGTATGAACTG11.282051282051282No Hit
TTTCAGAAGGATGCGAAAGTGCTTTTTCAAAATTGGGGAGTTGAACCTAT11.282051282051282No Hit
CACCATGGATACCAGTCGGGATATCCAGTATGGTGGAGGCTATGGTTTCC11.282051282051282No Hit
AATGGCAACTCAGCACCGTCTGGCCAAGACCAATCTACAGTATCACTATT11.282051282051282No Hit
AGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTCAATCC11.282051282051282No Hit
GTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTA11.282051282051282No Hit
CATAGGCAAATGGTGACAACAACCAATCCACTAATCAGACATGAGAACAG11.282051282051282No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGCGGGGGTGGGACGGGGGG11.282051282051282No Hit
ATCTTCATGATCTCAGTGAACTCTTCTTTCTTTATCCTTCCAGATTCGAA11.282051282051282No Hit
TTGCTAGCGACCTCAATTCTGGTTCATCACTATCATATTGCTTCAAATCA11.282051282051282No Hit
GGTATTTTCTCTGTTGAGGGCAAAAGCTGCATCAATAGGTGCTTTTATGT11.282051282051282No Hit
ATGCCCCAAAGTAGTGGAATACAGAAATTGGTCAAAGCCGCAATGTCAAA11.282051282051282No Hit
TAAAATGACCATGGCCTCTGTACCTGCGTCGCGTTACCTAACTGACATGA11.282051282051282No Hit
GTGGAACAGATCTTCATGATCTCAGTGAACTCTTCTTTCTTTATCCTTCC11.282051282051282No Hit
GATTATGGCCATATGGTCCACGGTGGTTTTTGTGAGTATCTCGCGGGTGC11.282051282051282No Hit
ACTAGAAGGATTTTCAGCTGAATCAAGAAAACTGCTTCTTATCGTTCAGG11.282051282051282No Hit
GTTATTATCTTTTGATTTGGATTCATTTTCACTCCTGCTTTTGCTCCTGT11.282051282051282No Hit
GTAATGAGAATGGGAGACCTCCACTCACTCCAAAACAGAAACGAGAAATG11.282051282051282No Hit
CCTCTGGGATTCCTTTCGTCAGTCCGAGAGAGGAGAAGAGACAATTGAAG11.282051282051282No Hit
ATGTATAGTTTGTCAAAATTGTCATTGTTTGGCATAGTCACGTTCAGCAC11.282051282051282No Hit
TTTTTACTCCAGCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAG11.282051282051282No Hit
CTTCAACAAATTGAGAGTATGATTGAAGCTGAGTCCTCTGTCAAAGAGAA11.282051282051282No Hit
TACCAGTACGTCTCCCGGCTTAACTATTGTCCAATAGATGCTTATTCTAC11.282051282051282No Hit
GCGTCGCGTTACCTAACTGACACGACTCTTGAGGAAACGTCAAGGGACTG11.282051282051282No Hit
CCACAGCACTCTGCTGTTCCTTTCGACATTCTTCCCTCATAGACTTTGGC11.282051282051282No Hit
GGTTGACCAAATCAGGAAGCACATATCCAGTGCTGAACGTGACTATGCCA11.282051282051282No Hit
TTAGAGGCCAATACAGTGGGTTTGTAAGAACTCTGTTCCAACAAATGAGG11.282051282051282No Hit
CCTCTATGGCATCGGTACGTGTACCTGTAATCTTTGATGAACAACTGAAG11.282051282051282No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGG11.282051282051282No Hit
ATATGATAGTGATGAACCAGAATTGAGGTCGCTAGCAAGTTGGATTCAGA11.282051282051282No Hit
ATCACTCACTGAGTGACATCAAAATCATGGCGTCCCAAGGCACCAAACGG11.282051282051282No Hit
GACAAGTCAAAGAGGAGTACTTGAAGATGAACAAAGGTGCTGCAATTTAT11.282051282051282No Hit
CACTTAGACTTCCGAATGGGCCTCCCTGTTCTCAGCGGTCCAAATTCCTG11.282051282051282No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCACAG50.0145.07
AAGAGTC50.0145.03
GAAGAGT50.0145.02
GAGTCAC50.0145.05
AGAGTCA50.0145.04
AATGGGA50.0145.0145
GGAAGAG50.0145.01
TCACAGT50.0145.08
CACAGTC50.0145.09
AGTCACA50.0145.06