FastQCFastQC Report
Mon 4 Feb 2019
000000000-BTF83_l01_n01_Exp3_HK_IAV_EG_E1_rep4_SID88.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BTF83_l01_n01_Exp3_HK_IAV_EG_E1_rep4_SID88.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences198183
Sequences flagged as poor quality0
Sequence length151
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3490.17609986729436936No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAG353.430465E-582.85714145
CGAAAGC1950.074.358973
AGCGAAA1900.072.51
GCGAAAG2100.065.595242
GAGCGAA1009.094947E-1265.251
CTCCGAC1051.6370905E-1162.142857145
CATCTCG753.7457175E-758.0145
TCGTATC651.0904296E-555.76923145
ATACACT858.888219E-751.17647145
CAAAAGC4550.050.9890144
CCCTCGA450.0096278248.3333364
CCCCGAC450.0096278248.333336145
ACGTGGA450.0096278248.3333365
GTGTAAC450.0096278248.3333361
CCACGGA803.7237536E-545.3125145
GAGCAAA5550.044.4144131
CCACGAA1151.4049147E-744.130436145
GCAAAAG5150.043.6407743
GTATTAT700.001054589241.428576
ACGGGCA700.001054589241.42857145
Produced by FastQC (version 0.11.5)