FastQCFastQC Report
Mon 4 Feb 2019
000000000-BTF83_l01_n01_Exp3_HK_IAV_EG_C1_rep3_SID87.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BTF83_l01_n01_Exp3_HK_IAV_EG_C1_rep3_SID87.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences128991
Sequences flagged as poor quality0
Sequence length151
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1290.10000697723096959No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAGCG100.007083753145.0145
GAGCGAA750.087.01
GCGGACG259.3680824E-487.0145
CTCCGGC353.425912E-582.85714145
CGCTCCC300.001931907172.56
CTCCGAC702.7866918E-972.49999145
CGAAAGC1300.061.3461573
AGCGAAA1350.059.0740741
CCACGAA902.0309017E-856.38889145
GCGAAAG1450.055.02
GCAAAAG3750.054.1333353
GAGCAAA4250.052.882351
CCGTATC553.2084805E-452.727276145
AGCAAAA4250.051.1764682
GTAGTCA450.00962025348.3333326
TGCCGCT604.9289654E-448.333332145
AAGCAGG5850.045.85477
CAAAAGC4750.045.7894744
ATACACT803.716472E-545.3125145
CATCTCG657.3127414E-444.615387145
Produced by FastQC (version 0.11.5)