FastQCFastQC Report
Mon 4 Feb 2019
000000000-BTF83_l01_n01_Exp3_HK_IAV_EG_B1_rep2_SID86.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BTF83_l01_n01_Exp3_HK_IAV_EG_B1_rep2_SID86.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences192941
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2520.130609875557813No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA1450.0100.01
CTCCGAC900.080.55556145
CTTATAG451.2291039E-680.555561
CTCGTAG406.649573E-572.5145
CCGAATC300.001933366272.5145
CGAAAGC2050.070.7317054
AGCGAAA2300.063.043482
GCGAAAG2450.059.1836743
CGTGGAG502.006161E-458.06
CGTGTAG502.006161E-458.05
CAGTACG502.006161E-458.09
CGTGCCA553.2124823E-452.7272765
ACGTGGA553.2124823E-452.7272765
TATCGTG553.2124823E-452.7272762
AGCCCCG858.886982E-751.17647145
AAGCAGG7650.048.3333367
GGTCGTT450.00962743848.3333327
CAGTCTA450.00962743848.3333329
TCGTGTA657.321811E-444.6153834
CAAAAGC5550.044.4144134
Produced by FastQC (version 0.11.5)