FastQCFastQC Report
Mon 4 Feb 2019
000000000-BTF83_l01_n01_Exp3_HK_IAV_EG_A1_rep1_SID85.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BTF83_l01_n01_Exp3_HK_IAV_EG_A1_rep1_SID85.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences182430
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2310.12662391054102945No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAC100.0070861434145.0145
CTCGTAG301.0980693E-7120.833336145
TATCGTG259.3743583E-487.02
TCTCCGC300.001933196972.5145
CGTGTAG300.001933196972.55
CAGTACG406.648603E-572.59
GAGCGAA651.3916542E-766.923071
CTCCGAC1051.6370905E-1162.142857145
TCGTGTA350.00356183162.1428574
CAAAAGC5550.058.7837834
GCAAAAG5550.058.7837833
CCACGGA502.0058696E-458.0145
ATCGTGT400.00604298854.3753
AGCGAAA1101.6625563E-952.7272721
TCGAGCA701.6904762E-551.7857139
AAAAGCA6700.050.858215
CGAAAGC1152.4647306E-950.4347843
GAGCAAA6600.049.4318161
CATCTCG450.00962660448.333332145
GGGATAC450.00962660448.3333323
Produced by FastQC (version 0.11.5)