FastQCFastQC Report
Mon 4 Feb 2019
000000000-BTF83_l01_n01_Exp3_HA_control_rep2_SID91.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BTF83_l01_n01_Exp3_HA_control_rep2_SID91.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences117359
Sequences flagged as poor quality0
Sequence length151
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATACATCATGGTCATAAGTTCCATTTCTGATTGACCCTATGCAGGCAT5420.461830792695916No Hit
GATGTATACAGAGATGAAGCATTAAACAACCGGTTCCAGATCAAAGGTGT3660.3118635980197514No Hit
GTATTATCCCGTGTTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACA3020.2573300726829642No Hit
GTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAG3010.2564779863495769No Hit
CTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTCCAAAATAA2820.24028834601521826No Hit
GGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAA2720.23176748268134528No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2600.2215424466806977No Hit
CATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATC2550.21728201501376118No Hit
TCTAATAACAGTTTCTTTAGTAGATTGAATTGGTTGACCCACTTAAAATT2400.20450072001295172No Hit
GTTATTAGATCTCCTTTTGCAAGCAGAGCTTGTTCCATTTTGAGTGACTC2350.2002402883460152No Hit
ACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAAT2310.19683194301246604No Hit
CTAAAATAGATCTCTGGTCATACAACGCGGAGCTTCTTGTTGCCCTGGAG2150.18319856167826923No Hit
ATCTGGGACAGGCCCCATATGTGATCCTGTTTACATTTTGAAATGGTTTG2120.18064230267810732No Hit
CCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTT2110.17979021634472006No Hit
ACATACAATTGATCTAACTGACTCAGAAATGAACAAACTGTTTGAAAGAA2110.17979021634472006No Hit
TAGTAAAACCGGGAGACATACTTTTGATTAACAGCACAGGGAATCTAATT2020.17212143934423435No Hit
CTATTGGACAATAGTAAAACCGGGAGACATACTTTTGATTAACAGCACAG1990.16956518034407245No Hit
TCATAAGGGTAACAGTTGCTGTGGGCTTTGCTGCGTTCAACAAAAAGGTC1980.16871309401068516No Hit
CATATGGGGCCTGTCCCAGATATGTTAAGCAAAACACTCTGAAATTGGCA1850.15763597167665028No Hit
TCTCAAAAGCACTCAAGCAGCAATCAACCAAATCAATGGGAAGCTGAATA1850.15763597167665028No Hit
GAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGG1780.1516713673429392No Hit
ATTTTAGTGTCCTCAACATATTTCTCGAGGTCCTGAATTCTCCCTTCTAC1750.1491151083427773No Hit
GTCCAATAGATGCTTATTCTGCTGGGGATATCCCTTACCCTGGGTCTAGA1750.1491151083427773No Hit
CTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGT1730.1474109356760027No Hit
CCCTTACCCTGGGTCTAGATCCGATATTCGGGATTACAGTTTGTTGGCTT1720.1465588493426154No Hit
ACATTCTATGTCTGGTTTTCGCTCAAAAACTTCCCGGAAATGACAACAGC1690.1440025903424535No Hit
ATTGTATGTTGGTTCTCCAGGGCAACAAGAAGCTCCGCGTTGTATGACCA1680.1431505040090662No Hit
TTCTATGTCTGGTTTTCGCTCAAAAACTTCCCGGAAATGACAACAGCACG1600.1363338133419678No Hit
ATCTATTAGTGTGCAGTTTTCTCCATCAAGGATCTGATGAGGACTGTCGC1580.1346296406751932No Hit
GATGAGGACTGTCGCATATTCCACCTGTTGAGGAACTCTGAACCAGCTCA1580.1346296406751932No Hit
TCTCTGTATACATCATGGTCATAAGTTCCATTTCTGATTGACCCTATGCA1560.13292546800841862No Hit
GTTTTTAATTAATGCACTCAAATGCAAATGTTGCACCTAATGTTGCCTTT1550.13207338167503133No Hit
TTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGT1520.12951712267486942No Hit
TCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATAC1500.12781295000809484No Hit
GAATAGGACAAGCAGCAGATCTCAAAAGCACTCAAGCAGCAATCAACCAA1490.12696086367470752No Hit
GTCCCAGATATGTTAAGCAAAACACTCTGAAATTGGCAACAGGGATGCGA1470.12525669100793294No Hit
CTATTGGGAGACCCTCAGTGTGATGGCTTCCAAAATAAGAAATGGGACCT1470.12525669100793294No Hit
CTGTATACATCATGGTCATAAGTTCCATTTCTGATTGACCCTATGCAGGC1450.12355251834115834No Hit
CTATTTTAGTGTCCTCAACATATTTCTCGAGGTCCTGAATTCTCCCTTCT1440.12270043200777103No Hit
GGGTAAGGGATATCCCCAGCAGAATAAGCATCTATTGGACAATAGTAAAA1430.12184834567438373No Hit
GCATTAATGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGC1410.12014417300760913No Hit
ACCCTATGCAGGCATTGTCACATTTGTGGTATATTTTGAAACAACCATTG1400.11929208667422182No Hit
CCCTTATGATGTGCCGGATTATGCCTCCCTTAGGTCACTAGTTGCCTCAT1400.11929208667422182No Hit
GTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGT1380.11758791400744723No Hit
GCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCA1370.11673582767405992No Hit
ATGTGATCCTGTTTACATTTTGAAATGGTTTGTCATTGGGAATGCTTCCA1370.11673582767405992No Hit
ATACAGAGATGAAGCATTAAACAACCGGTTCCAGATCAAAGGTGTTGAGC1370.11673582767405992No Hit
GTCCGTACCCGGGTGGTGAACTCCCCAAATGTACAATTTGTCAAAATTTT1360.11588374134067264No Hit
CTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGA1350.11503165500728534No Hit
GTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGC1350.11503165500728534No Hit
CTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCA1330.11332748234051074No Hit
GGTATATCTTTCGCTCCGAGTCGGCATTTTGGGCCGCCGGGTTATTAGTA1310.11162330967373615No Hit
CACTAAAATAGATCTCTGGTCATACAACGCGGAGCTTCTTGTTGCCCTGG1310.11162330967373615No Hit
GTTTTACTATTGTCCAATAGATGCTTATTCTGCTGGGGATATCCCTTACC1310.11162330967373615No Hit
GTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCAT1310.11162330967373615No Hit
CCCATATCCTCAGCATTTTCCCTCAGTTGCTTCTTTGTTCTTTCAAACAG1300.11077122334034885No Hit
CTATTAACCATGAAGACTATCTTTGCTTTGAGCTACATTCTATGTCTGGT1300.11077122334034885No Hit
ATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTG1290.10991913700696154No Hit
ATGCAGGCATTGTCACATTTGTGGTATATTTTGAAACAACCATTGCCCAT1280.10906705067357425No Hit
GGTACGGACAATGACCAAATCAGCCTATATGCTCAAGCATCAGGAAGAAT1270.10821496434018694No Hit
GATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACAT1270.10821496434018694No Hit
GATCTAGACCCAGGGTAAGGGATATCCCCAGCAGAATAAGCATCTATTGG1260.10736287800679964No Hit
GTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCT1240.10565870534002504No Hit
GTCATTGGGAATGCTTCCATTTGGAGTGATGCATTCAGAATTGCATTTGC1230.10480661900663776No Hit
GTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTC1220.10395453267325046No Hit
CTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGG1220.10395453267325046No Hit
GTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACT1220.10395453267325046No Hit
ACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATA1220.10395453267325046No Hit
ATTAGATTCCCTGTGCTGTTAATCAAAAGTATGTCTCCCGGTTTTACTAT1220.10395453267325046No Hit
GCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAG1200.10225036000647586No Hit
TCTATTAGTGTGCAGTTTTCTCCATCAAGGATCTGATGAGGACTGTCGCA1190.10139827367308855No Hit
GTCCTGGGGTTGACCAGAGGGCCCCGGGCGCTCCGTGTGTGGCTGCGATG1180.10054618733970125No Hit
GTCCTGAATTCTCCCTTCTACTTCTGAGAATTCTTTTTCAATCTGATGGA1180.10054618733970125No Hit
ATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTT1180.10054618733970125No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACAT151.2272618E-4145.05
GAGTTAT100.007082946145.01
TTACACT202.1226952E-6145.0145
TGCTTGG203.8574662E-4108.75145
CTCTTAA203.8574662E-4108.75145
TCGGACA203.8574662E-4108.754
ATGAGAG203.8574662E-4108.752
GAGAAGA203.8574662E-4108.756
GACATCT203.8574662E-4108.757
TTCGGAC203.8574662E-4108.753
GGACATC203.8574662E-4108.756
TGAGAGA203.8574662E-4108.753
TTTCGGA259.365966E-487.02
GATGAGA259.365966E-487.01
ATAACCG259.365966E-487.0145
TCGTATC353.4246223E-582.85715145
CTGTCCT406.638729E-572.5145
CTCTTTA406.638729E-572.5145
AGCCCCG350.003558665362.14286145
GAGAGAA350.003558665362.142864
Produced by FastQC (version 0.11.5)