FastQCFastQC Report
Mon 4 Feb 2019
000000000-BTF83_l01_n01_Exp3_HA_control_rep1_SID89.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BTF83_l01_n01_Exp3_HA_control_rep1_SID89.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences118383
Sequences flagged as poor quality0
Sequence length151
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATACATCATGGTCATAAGTTCCATTTCTGATTGACCCTATGCAGGCAT8350.7053377596445436No Hit
GATGTATACAGAGATGAAGCATTAAACAACCGGTTCCAGATCAAAGGTGT5190.43840754162337503No Hit
CTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTCCAAAATAA3730.3150790231705566No Hit
GGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAA3670.31001072789167367No Hit
GTATTATCCCGTGTTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACA3540.2990294214540939No Hit
ATCTGGGACAGGCCCCATATGTGATCCTGTTTACATTTTGAAATGGTTTG3430.28973754677614183No Hit
GTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAG3400.28720339913670034No Hit
CTAAAATAGATCTCTGGTCATACAACGCGGAGCTTCTTGTTGCCCTGGAG3190.26946436566061005No Hit
CCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTT2970.25088061630470593No Hit
GTTATTAGATCTCCTTTTGCAAGCAGAGCTTGTTCCATTTTGAGTGACTC2800.2365204463478709No Hit
TCATAAGGGTAACAGTTGCTGTGGGCTTTGCTGCGTTCAACAAAAAGGTC2770.23398629870842944No Hit
ACATACAATTGATCTAACTGACTCAGAAATGAACAAACTGTTTGAAAGAA2750.23229686694880178No Hit
TCTAATAACAGTTTCTTTAGTAGATTGAATTGGTTGACCCACTTAAAATT2740.23145215106898798No Hit
ACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAAT2660.22469442403047735No Hit
CATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATC2460.20780010643420085No Hit
CTGTATACATCATGGTCATAAGTTCCATTTCTGATTGACCCTATGCAGGC2430.2052659587947594No Hit
CTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGT2380.20104237939569028No Hit
ATCTATTAGTGTGCAGTTTTCTCCATCAAGGATCTGATGAGGACTGTCGC2310.19512936823699348No Hit
TCTCTGTATACATCATGGTCATAAGTTCCATTTCTGATTGACCCTATGCA2210.18668220943885525No Hit
ACATTCTATGTCTGGTTTTCGCTCAAAAACTTCCCGGAAATGACAACAGC2170.18330334591959993No Hit
ATTGTATGTTGGTTCTCCAGGGCAACAAGAAGCTCCGCGTTGTATGACCA2090.17654561888108936No Hit
GTCCAATAGATGCTTATTCTGCTGGGGATATCCCTTACCCTGGGTCTAGA2090.17654561888108936No Hit
CTATTGGACAATAGTAAAACCGGGAGACATACTTTTGATTAACAGCACAG2020.17063260772239258No Hit
GTTTTTAATTAATGCACTCAAATGCAAATGTTGCACCTAATGTTGCCTTT1940.16387488068388198No Hit
TCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATAC1910.1613407330444405No Hit
ATGCAGGCATTGTCACATTTGTGGTATATTTTGAAACAACCATTGCCCAT1850.15627243776555755No Hit
CTATTGGGAGACCCTCAGTGTGATGGCTTCCAAAATAAGAAATGGGACCT1840.15542772188574375No Hit
CCCTTACCCTGGGTCTAGATCCGATATTCGGGATTACAGTTTGTTGGCTT1790.1512041424866746No Hit
GCATTAATGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGC1760.14866999484723312No Hit
TCTCAAAAGCACTCAAGCAGCAATCAACCAAATCAATGGGAAGCTGAATA1750.1478252789674193No Hit
ATACAGAGATGAAGCATTAAACAACCGGTTCCAGATCAAAGGTGTTGAGC1750.1478252789674193No Hit
CATATGGGGCCTGTCCCAGATATGTTAAGCAAAACACTCTGAAATTGGCA1710.144446415448164No Hit
GAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGG1710.144446415448164No Hit
GTTTTACTATTGTCCAATAGATGCTTATTCTGCTGGGGATATCCCTTACC1690.14275698368853637No Hit
ATGTGATCCTGTTTACATTTTGAAATGGTTTGTCATTGGGAATGCTTCCA1680.14191226780872254No Hit
CTATTAACCATGAAGACTATCTTTGCTTTGAGCTACATTCTATGTCTGGT1680.14191226780872254No Hit
ATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTG1660.14022283604909488No Hit
GATGAGGACTGTCGCATATTCCACCTGTTGAGGAACTCTGAACCAGCTCA1660.14022283604909488No Hit
ATTTTAGTGTCCTCAACATATTTCTCGAGGTCCTGAATTCTCCCTTCTAC1650.13937812016928106No Hit
TAGTAAAACCGGGAGACATACTTTTGATTAACAGCACAGGGAATCTAATT1640.13853340428946725No Hit
ATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAAT1600.13515454077021194No Hit
CAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCG1570.13262039313077048No Hit
ACTAAAGAAACTGTTATTAGATCTCCTTTTGCAAGCAGAGCTTGTTCCAT1560.13177567725095662No Hit
GGTACGGACAATGACCAAATCAGCCTATATGCTCAAGCATCAGGAAGAAT1530.12924152961151517No Hit
CTATTTTAGTGTCCTCAACATATTTCTCGAGGTCCTGAATTCTCCCTTCT1500.12670738197207368No Hit
GTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATA1500.12670738197207368No Hit
CACTAAAATAGATCTCTGGTCATACAACGCGGAGCTTCTTGTTGCCCTGG1490.12586266609225988No Hit
GTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCT1490.12586266609225988No Hit
AACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAG1460.12332851845281839No Hit
TTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATT1460.12332851845281839No Hit
GTATACAGAGATGAAGCATTAAACAACCGGTTCCAGATCAAAGGTGTTGA1450.12248380257300458No Hit
GGTATATCTTTCGCTCCGAGTCGGCATTTTGGGCCGCCGGGTTATTAGTA1440.12163908669319075No Hit
CTACCAAAAGAAGCCAACAAACTGTAATCCCGAATATCGGATCTAGACCC1430.12079437081337692No Hit
GTCCCAGATATGTTAAGCAAAACACTCTGAAATTGGCAACAGGGATGCGA1410.11910493905374928No Hit
TTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGT1410.11910493905374928No Hit
GTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACT1410.11910493905374928No Hit
ATGCAGTACCAAACGGAACGATAGTGAAAACAATCACGAATGACCAAATT1400.11826022317393545No Hit
CTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGA1400.11826022317393545No Hit
CCCTTATGATGTGCCGGATTATGCCTCCCTTAGGTCACTAGTTGCCTCAT1400.11826022317393545No Hit
ATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTT1390.11741550729412163No Hit
ACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATA1380.1165707914143078No Hit
TCTATTAGTGTGCAGTTTTCTCCATCAAGGATCTGATGAGGACTGTCGCA1370.11572607553449399No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1360.11488135965468016No Hit
TTCTATGTCTGGTTTTCGCTCAAAAACTTCCCGGAAATGACAACAGCACG1360.11488135965468016No Hit
GTCCGTACCCGGGTGGTGAACTCCCCAAATGTACAATTTGTCAAAATTTT1350.11403664377486632No Hit
ACCCTATGCAGGCATTGTCACATTTGTGGTATATTTTGAAACAACCATTG1350.11403664377486632No Hit
ATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGC1340.11319192789505249No Hit
TCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTG1330.11234721201523867No Hit
ATAGTAAAACCGGGAGACATACTTTTGATTAACAGCACAGGGAATCTAAT1330.11234721201523867No Hit
CTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCA1330.11234721201523867No Hit
ATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTT1330.11234721201523867No Hit
GAACGTGACTATGCCAAACAATGAAAATTTTGACAAATTGTACATTTGGG1320.11150249613542484No Hit
GATCTAGACCCAGGGTAAGGGATATCCCCAGCAGAATAAGCATCTATTGG1320.11150249613542484No Hit
CACTAATAGATGCTCTATTGGGAGACCCTCAGTGTGATGGCTTCCAAAAT1320.11150249613542484No Hit
TTCTTTAGTAGATTGAATTGGTTGACCCACTTAAAATTCAAATACCCAGC1310.11065778025561102No Hit
TGTATACAGAGATGAAGCATTAAACAACCGGTTCCAGATCAAAGGTGTTG1310.11065778025561102No Hit
ATTGGGAGACCCTCAGTGTGATGGCTTCCAAAATAAGAAATGGGACCTTT1310.11065778025561102No Hit
GGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTC1290.10896834849598337No Hit
CTATTAGTGTGCAGTTTTCTCCATCAAGGATCTGATGAGGACTGTCGCAT1290.10896834849598337No Hit
GCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAG1290.10896834849598337No Hit
GTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCAT1290.10896834849598337No Hit
GGATCACATATGGGGCCTGTCCCAGATATGTTAAGCAAAACACTCTGAAA1270.10727891673635573No Hit
TATGGGGCCTGTCCCAGATATGTTAAGCAAAACACTCTGAAATTGGCAAC1270.10727891673635573No Hit
ACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGA1260.1064342008565419No Hit
GTCCTGGGGTTGACCAGAGGGCCCCGGGCGCTCCGTGTGTGGCTGCGATG1260.1064342008565419No Hit
GTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGT1250.10558948497672807No Hit
GAATAGGACAAGCAGCAGATCTCAAAAGCACTCAAGCAGCAATCAACCAA1240.10474476909691426No Hit
GATCTAACTGACTCAGAAATGAACAAACTGTTTGAAAGAACAAAGAAGCA1220.10305533733728661No Hit
CCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGG1220.10305533733728661No Hit
GCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTG1210.10221062145747278No Hit
TCCCAATAGAGCATCTATTAGTGTGCAGTTTTCTCCATCAAGGATCTGAT1200.10136590557765895No Hit
GGGTAAGGGATATCCCCAGCAGAATAAGCATCTATTGGACAATAGTAAAA1200.10136590557765895No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCAT100.007083022145.0145
TATGACA100.007083022145.0145
CTCGAAG100.007083022145.0145
GGACATA100.007083022145.0145
ACCATGG301.0956501E-7120.83332145
CTCTTAA301.0956501E-7120.83332145
TCTCGTG256.441165E-6116.0145
ACAATTC203.8575486E-4108.75145
GCGGGTA203.8575486E-4108.75145
CGAGCGA203.8575486E-4108.75145
CACATCC651.3874342E-766.92307145
CTGTCCT350.003558740762.142857145
CATCTCG350.003558740762.142857145
TACATCA3100.058.4677434
CCCATCC502.002978E-458.0145
CATGGTC3200.056.6406259
ATCATGG3250.055.769237
TATACAT3400.053.3088262
ATACATC3400.053.3088263
TATTATC1850.047.027032
Produced by FastQC (version 0.11.5)