FastQCFastQC Report
Mon 4 Feb 2019
000000000-BTF83_l01_n01_Exp3_CW_B03_SID82.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BTF83_l01_n01_Exp3_CW_B03_SID82.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences205814
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTCAAATATATTCAATATGGAAAGAATAAAAGAACTAA27911.3560787895867141No Hit
TTTTTAAACTATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG19540.9494009153896237No Hit
AATATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCA13710.6661354426812559No Hit
AGCGAAAGCAGGTCAAATATATTCAATATGGAAAGAATAAAAGAACTAAG9820.4771298356768733No Hit
TCCTCATTCTGGGCAAAGAGGACAAGAGATATGGGCCAGCACTAAGCATC9480.4606100653988553No Hit
GAATAAAAGAACTAAGAAATCTAATGTCGCAGTCTCGCACCCGCGAGATA8830.4290281516320561No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT8150.39598861107602007No Hit
GAGCGAAAGCAGGTCAAATATATTCAATATGGAAAGAATAAAAGAACTAA7830.38044059199082664No Hit
CAGTAAGTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACCACATCC7820.37995471639441436No Hit
GTAGAAACAAGGTCGTTTTTAAACTATTCGACACTAATTGATGGCCATCC7590.3687795776769316No Hit
AAACAAGGTCGTTTTTAAACTATTCGACACTAATTGATGGCCATCCGAAT7580.3682937020805193No Hit
TCAATGAACTGAGCAACCTTGCGAAAGGAGAGAAGGCTAATGTGCTAATT7190.34934455382043983No Hit
GTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACCACATCCCCTTGC6940.33719766391013245No Hit
GGTCAAATATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATG6780.32942365436753573No Hit
GAATGAGGAATCCCCTCAGTCTTCTCCTGTCTTCCTGATGTGTACTTCTT6260.3041581233540964No Hit
GCAAAAGCAGGTCAAATATATTCAATATGGAAAGAATAAAAGAACTAAGA6190.3007569941792103No Hit
ATCTAATGTCGCAGTCTCGCACCCGCGAGATACTCACAAAAACCACCGTG6130.2978417406007366No Hit
AGCAAAAGCAGGTCAAATATATTCAATATGGAAAGAATAAAAGAACTAAG5620.27306208518370956No Hit
GTTCATTGATGCTTAGTGCTGGCCCATATCTCTTGTCCTCTTTGCCCAGA5540.2691750804124112No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT5520.2682033292195866No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA5080.24682480297744563No Hit
GGAGAAGACTGAGGGGATTCCTCATTCTGGGCAAAGAGGACAAGAGATAT5000.24293779820614728No Hit
ATCAAGAAGTACACATCAGGAAGACAGGAGAAGACTGAGGGGATTCCTCA4860.23613553985637517No Hit
ACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG4850.2356496642599629No Hit
GTCTTCTCCTGTCTTCCTGATGTGTACTTCTTGATTATGGCCATATGGTC4800.23322028627790142No Hit
ATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCAGTC4620.2244745255424801No Hit
GTGCTGGCCCATATCTCTTGTCCTCTTTGCCCAGAATGAGGAATCCCCTC4540.22058752077118174No Hit
CTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAA4400.2137852624214096No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG4400.2137852624214096No Hit
GTCTCGCACCCGCGAGATACTCACAAAAACCACCGTGGACCATATGGCCA4350.21135588443934816No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4230.2055253772824006No Hit
GGCCAGCACTAAGCATCAATGAACTGAGCAACCTTGCGAAAGGAGAGAAG4020.19532198975774243No Hit
AGTAGAAACAAGGTCGTTTTTAAACTATTCGACACTAATTGATGGCCATC3960.19240673617926865No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACCACAT3860.1875479802151457No Hit
ATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCAGTCTC3840.18657622902232113No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT3670.17831634388331213No Hit
GCTTAGTGCTGGCCCATATCTCTTGTCCTCTTTGCCCAGAATGAGGAATC3650.17734459269048752No Hit
GCACTAAGCATCAATGAACTGAGCAACCTTGCGAAAGGAGAGAAGGCTAA3630.17637284149766294No Hit
AAACAAGGGTGTTTTTTATTATTAAATAAGCTGAAACGAGAAAGTTCTTA3320.16131069800888181No Hit
CCACATCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTCGCAAGG3180.15450843965910968No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT3110.15110731048422363No Hit
ATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCAGTCTCGCACC3110.15110731048422363No Hit
TCGCAAGGTTGCTCAGTTCATTGATGCTTAGTGCTGGCCCATATCTCTTG3070.14916380809857444No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA3070.14916380809857444No Hit
CCATATGGCCATAATCAAGAAGTACACATCAGGAAGACAGGAGAAGACTG2920.14187567415239002No Hit
AATTAGCACATTAGCCTTCTCTCCTTTCGCAAGGTTGCTCAGTTCATTGA2900.14090392295956544No Hit
CTTCTTGATTATGGCCATATGGTCCACGGTGGTTTTTGTGAGTATCTCGC2890.14041804736315314No Hit
CTCTTGTCCTCTTTGCCCAGAATGAGGAATCCCCTCAGTCTTCTCCTGTC2890.14041804736315314No Hit
ATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACCACATCCCCTTGCCC2800.13604516699544247No Hit
GAGCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAAACCTACT2780.13507341580261792No Hit
GTACACATCAGGAAGACAGGAGAAGACTGAGGGGATTCCTCATTCTGGGC2710.13167228662773184No Hit
ACCAACACCACATCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTT2710.13167228662773184No Hit
CACCACATCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTCGCAA2680.13021465983849495No Hit
ATATACAACAGGATGGGGGCTGTGACCACTGAAGTGGCATTTGGCCTGGT2640.12827115745284579No Hit
CATTAGCCTTCTCTCCTTTCGCAAGGTTGCTCAGTTCATTGATGCTTAGT2640.12827115745284579No Hit
GTGTTATCATTCCATTCAAGTCCCCCGATGAGGACTCCAACTGCATTTTT2600.1263276550671966No Hit
CATCAGGAAGACAGGAGAAGACTGAGGGGATTCCTCATTCTGGGCAAAGA2580.125355903874372No Hit
ATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTA2540.12341240148872282No Hit
CTATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGG2450.11903952112101218No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC2450.11903952112101218No Hit
CTCCAGCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCACTCT2450.11903952112101218No Hit
TATCTCTTGTCCTCTTTGCCCAGAATGAGGAATCCCCTCAGTCTTCTCCT2440.11855364552459988No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGATCCCCGTTCCC2380.11563839194612613No Hit
TCCTAGCTCCAGTGCTGGTCTGAAAAATGATCTTCTTGAAAATTTGCAGG2350.11418076515688923No Hit
TACTCACTGACAGCCAGACAGCGACCAAAAGAATTCGGATGGCCATCAAT2310.11223726277124005No Hit
TTCCTGATGTGTACTTCTTGATTATGGCCATATGGTCCACGGTGGTTTTT2310.11223726277124005No Hit
TCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCC2200.1068926312107048No Hit
GGTATGTGCAACCTGTGAACAGATTGCTGACTCCCAGCATCGGTCTCATA2200.1068926312107048No Hit
CAGTGAGTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACCACATCC2200.1068926312107048No Hit
CTCTAATATTGCTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAA2190.10640675561429253No Hit
GATGTGTACTTCTTGATTATGGCCATATGGTCCACGGTGGTTTTTGTGAG2180.10592088001788022No Hit
GTGCTAATTGGGCAAGGGGATGTGGTGTTGGTAATGAAACGGAAACGGGA2170.10543500442146793No Hit
GAGCAAAAGCAGGGTAGATAATCACTCACTGAGTGACATCAAAATCATGG2160.10494912882505562No Hit
ACCTTGCGAAAGGAGAGAAGGCTAATGTGCTAATTGGGCAAGGGGATGTG2140.10397737763223104No Hit
GAACTAAGAAATCTAATGTCGCAGTCTCGCACCCGCGAGATACTCACAAA2140.10397737763223104No Hit
ATTAGCACATTAGCCTTCTCTCCTTTCGCAAGGTTGCTCAGTTCATTGAT2130.10349150203581875No Hit
GCCATATGGTCCACGGTGGTTTTTGTGAGTATCTCGCGGGTGCGAGACTG2060.10009037286093268No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTGCG100.0070868004145.0145
GCGCCTT100.0070868004145.0145
GCATTCG100.0070868004145.0145
CGAAGCG100.0070868004145.0145
GAGCGAA1450.0120.01
AGCAAAA6450.096.6666642
CTGCTTA700.093.21429145
GCAAAAG6950.091.798563
TCTCCGC259.37608E-487.0145
GGACAGT353.43078E-582.857146
AGCCCAG801.8189894E-1281.5625145
AGTAGAA2400.081.56251
CTCCGAC900.080.55556145
AGCAGGC451.2293967E-680.555568
AGCGAAA3000.077.3333361
GCAGGTC5950.076.764719
TTGCTCG300.00193355172.5145
TTTCCTA406.6506305E-572.5145
GGCGAAC300.00193355172.5145
CCACGAA809.822543E-1172.5145
Produced by FastQC (version 0.11.5)