FastQCFastQC Report
Mon 4 Feb 2019
000000000-BTF83_l01_n01_Exp2_SrcID47_NL_Cnt5_D4_rep2_SID74.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BTF83_l01_n01_Exp2_SrcID47_NL_Cnt5_D4_rep2_SID74.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences170745
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTCAATCC15710.9200855076283346No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT13040.7637119681396234No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAA9760.5716126387302704No Hit
GTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTA9320.5458432164924303No Hit
GAGCAAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAA7470.4374945093560572No Hit
TTTTTAAACTATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG7360.4310521537965972No Hit
ATTATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCA6430.37658496588479895No Hit
TTTCAAATCTGTGCTTCAAAAGTGCATTTGGATCACCAAGTTCTACGATT6390.3742422911359044No Hit
AGTCAGTATTCAACAGCTTGTATGCATCTCCACAACTAGAAGGATTTTCA5950.3484728688980644No Hit
ATACCGACTTTGCTAATATTTCAAATCTGTGCTTCAAAAGTGCATTTGGA5550.3250461214091188No Hit
CTTAGGGACAACCTTGAACCTGGGACCTTTGATCTTGGGGGGCTATATGA5230.30630472341796244No Hit
ATATGAAGCAATTGAGGAGTGCCTGATTAATGATCCCTGGGTTTTGCTTA5080.2975196931096079No Hit
TAGTAGCACTGCCACAACTAACTCAATGCATGTGTAAGGAAGGAGTTGAA4660.27292160824621514No Hit
CACTTGGAAGTATGCTTCATGTATTCAGATTTTCACTTCATCAATGAGCA4300.25183753550616417No Hit
TCGTAGAACTTGGTGATCCAAATGCACTTTTGAAGCACAGATTTGAAATA4280.25066619813171687No Hit
GCTTCATATAGCCCCCCAAGATCAAAGGTCCCAGGTTCAAGGTTGTCCCT4010.2348531435766787No Hit
CTTCCAAGTGAGTGCATATTGCTGCAAATTTGTTTGTTTCGATTTTCAGG3880.22723945064277137No Hit
GTATGGGGAGGACCTGAAAATCGAAACAAACAAATTTGCAGCAATATGCA3760.22021142639608773No Hit
ACCTTGAACCTGGGACCTTTGATCTTGGGGGGCTATATGAAGCAATTGAG3710.21728308295996956No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3660.21435473952385137No Hit
GAATAAAAGAACTAAGAAATCTAATGTCGCAGTCTCGCACCCGCGAGATA3660.21435473952385137No Hit
AGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCAC3600.21084072740050952No Hit
GTTCAAGGTTGTCCCTAAGAGCCTGAACGATAAGAAGCAGTTTTCTTGAT3580.20966939002606227No Hit
AGTATGGGGAGGACCTGAAAATCGAAACAAACAAATTTGCAGCAATATGC3550.20791238396439135No Hit
TCCTCATTCTGGGCAAAGAAGACAAGAGATATGGGCCAGCACTAAGCATC3540.2073267152771677No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT3480.20381270315382588No Hit
GTATTCAACAGCTTGTATGCATCTCCACAACTAGAAGGATTTTCAGCTGA3450.20205569709215496No Hit
GTAGAAACAAGGTCGTTTTTAAACTATTCGACACTAATTGATGGCCATCC3320.19444200415824767No Hit
ATCTAATGTCGCAGTCTCGCACCCGCGAGATACTCACAAAAACCACCGTG3310.19385633547102404No Hit
AGCGAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTT3270.1915136607221295No Hit
GAATGAGGAATCCCCTCAGAACAGCGGACTCCACTCCAGCTGTGCCTTCA3230.18917098597323495No Hit
GTCCCTAAGAGCCTGAACGATAAGAAGCAGTTTTCTTGATTCAGCTGAAA3200.18741397991156403No Hit
GAAGCAGTTTTCTTGATTCAGCTGAAAATCCTTCTAGTTGTGGAGATGCA3190.1868283112243404No Hit
ATATAGCCCCCCAAGATCAAAGGTCCCAGGTTCAAGGTTGTCCCTAAGAG3110.18214296172655128No Hit
TCATTAATCAGGCACTCCTCAATTGCTTCATATAGCCCCCCAAGATCAAA3100.18155729303932763No Hit
GTGTAAGGAAGGAGTTGAACCAAGAAGCATTAAGCAAAACCCAGGGATCA3100.18155729303932763No Hit
AGCGAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAAG3070.17980028697765674No Hit
TCAATGAACTGAGCAACCTTGCGAAAGGAGAGAAGGCTAATTGGGCAAGG3040.1780432809159858No Hit
GGTTCAAGGTTGTCCCTAAGAGCCTGAACGATAAGAAGCAGTTTTCTTGA3000.17570060616709127No Hit
ATTGAGGAGTGCCTGATTAATGATCCCTGGGTTTTGCTTAATGCTTCTTG2970.17394360010542037No Hit
GTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCACT2960.17335793141819672No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT2920.17101525666930217No Hit
GCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTC2880.16867258192040763No Hit
AAACAAGGTCGTTTTTAAACTATTCGACACTAATTGATGGCCATCCGAAT2880.16867258192040763No Hit
CTCCTCAATTGCTTCATATAGCCCCCCAAGATCAAAGGTCCCAGGTTCAA2860.16750124454596035No Hit
GCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTAAGGAAGGAG2860.16750124454596035No Hit
GCCACAACTAACTCAATGCATGTGTAAGGAAGGAGTTGAACCAAGAAGCA2820.1651585697970658No Hit
AACTAACTCAATGCATGTGTAAGGAAGGAGTTGAACCAAGAAGCATTAAG2810.16457290110984216No Hit
GAGCAAGGCGAGTCAATAATCGTAGAACTTGGTGATCCAAATGCACTTTT2790.16340156373539488No Hit
TCAATGAGCAAGGCGAGTCAATAATCGTAGAACTTGGTGATCCAAATGCA2770.1622302263609476No Hit
GTTCTACGATTATTGACTCGCCTTGCTCATTGATGAAGTGAAAATCTGAA2760.161644557673724No Hit
GTATGCATCTCCACAACTAGAAGGATTTTCAGCTGAATCAAGAAAACTGC2730.15988755161205306No Hit
GCATTAAGCAAAACCCAGGGATCATTAATCAGGCACTCCTCAATTGCTTC2710.1587162142376058No Hit
ATTATTGACTCGCCTTGCTCATTGATGAAGTGAAAATCTGAATACATGAA2700.15813054555038217No Hit
ATAAGAAGCAGTTTTCTTGATTCAGCTGAAAATCCTTCTAGTTGTGGAGA2620.15344519605259305No Hit
GTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACCACGTCTCCTTGC2550.1493455152420276No Hit
ATCAGGCACTCCTCAATTGCTTCATATAGCCCCCCAAGATCAAAGGTCCC2530.1481741778675803No Hit
GCTTCAATCCGATGATTGTCGAGCTTGCGGAAAAAACAATGAAAGAGTAT2520.14758850918035665No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG2510.14700284049313303No Hit
GAGCGAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT2460.14407449705701483No Hit
CTTGGAAGTATGCTTCATGTATTCAGATTTTCACTTCATCAATGAGCAAG2370.13880347887200212No Hit
ATCAAGAAGTACACATCAGGAAGACAGGAGAAGAACCCAGCACTTAGGAT2360.13821781018477847No Hit
GATTTGAAATATTAGCAAAGTCGGTATTCAACAGCTTGTATGCATCTCCA2300.13470379806143665No Hit
CCTCAATTGCTTCATATAGCCCCCCAAGATCAAAGGTCCCAGGTTCAAGG2220.13001844856364755No Hit
GTTCTGAGGGGATTCCTCATTCTGGGCAAAGAAGACAAGAGATATGGGCC2210.1294327798764239No Hit
GAGTATGGGGAGGACCTGAAAATCGAAACAAACAAATTTGCAGCAATATG2180.127675773814753No Hit
GTCGGTATTCAACAGCTTGTATGCATCTCCACAACTAGAAGGATTTTCAG2140.12533309906585843No Hit
GATCAAAGGTCCCAGGTTCAAGGTTGTCCCTAAGAGCCTGAACGATAAGA2110.12357609300418754No Hit
CCTCAGAACAGCGGACTCCACTCCAGCTGTGCCTTCATCTGGGTCTTCAG2100.12299042431696389No Hit
CCTTGAACCTGGGACCTTTGATCTTGGGGGGCTATATGAAGCAATTGAGG2100.12299042431696389No Hit
ACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG2070.12123341825529298No Hit
GTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTCAATCCGATGA2050.12006208088084569No Hit
AGTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGT2040.11947641219362207No Hit
GCTTGTATGCATCTCCACAACTAGAAGGATTTTCAGCTGAATCAAGAAAA2030.11889074350639843No Hit
CTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAA1980.11596240007028023No Hit
GGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATG1950.11420539400860932No Hit
CAACTAACTCAATGCATGTGTAAGGAAGGAGTTGAACCAAGAAGCATTAA1940.11361972532138569No Hit
GTTCATTGATGCTTAGTGCTGGCCCATATCTCTTGTCTTCTTTGCCCAGA1940.11361972532138569No Hit
ACAATGCTTCAATCCGATGATTGTCGAGCTTGCGGAAAAAACAATGAAAG1940.11361972532138569No Hit
TCCCCATACTCTTTCATTGTTTTTTCCGCAAGCTCGACAATCATCGGATT1930.11303405663416206No Hit
AGTAGAAACAAGGTCGTTTTTAAACTATTCGACACTAATTGATGGCCATC1920.11244838794693841No Hit
ATACTGACTTTGCTAATATTTCAAATCTGTGCTTCAAAAGTGCATTTGGA1900.11127705057249115No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT1880.11010571319804387No Hit
GAGCGAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAA1850.10834870713637296No Hit
TGTGTAAGGAAGGAGTTGAACCAAGAAGCATTAAGCAAAACCCAGGGATC1850.10834870713637296No Hit
TCATCAATGAGCAAGGCGAGTCAATAATCGTAGAACTTGGTGATCCAAAT1830.10717736976192568No Hit
GTGCTGGCCCATATCTCTTGTCTTCTTTGCCCAGAATGAGGAATCCCCTC1830.10717736976192568No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA1790.10483469501303114No Hit
ACTTCCAAGTGAGTGCATATTGCTGCAAATTTGTTTGTTTCGATTTTCAG1790.10483469501303114No Hit
ATGGAAGATTTTGTGCGACAATGCTTCAATCCGATGATTGTCGAGCTTGC1770.10366335763858385No Hit
ATTCAACAGCTTGTATGCATCTCCACAACTAGAAGGATTTTCAGCTGAAT1770.10366335763858385No Hit
GGCCAGCACTAAGCATCAATGAACTGAGCAACCTTGCGAAAGGAGAGAAG1740.10190635157691294No Hit
ACCCAGGGATCATTAATCAGGCACTCCTCAATTGCTTCATATAGCCCCCC1720.10073501420246568No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTGCG100.0070857485145.0145
TAGTGCG100.0070857485145.0145
CGAGACG600.0120.833336145
CCCCGAC256.449405E-6116.0145
GAGCGAA1150.0107.1739041
AGCAGGC352.751858E-7103.571438
CACGAGC301.5956433E-596.666664145
GCAAAAG4750.096.15793
GCAGGGT1100.092.2727369
AGCCCAG750.087.0145
TAGTTGG259.3733217E-487.0145
TTAACTA259.3733217E-487.0145
TCTCCGC259.3733217E-487.0145
TCGTATC608.240022E-1084.583336145
AAGCAGG6900.083.007257
GAGCAAA5700.080.131581
TCGTACT554.3724867E-879.090912
CAAAAGC5700.078.859654
ATACACT903.6379788E-1272.50001145
CTACTAG300.001932983872.51
Produced by FastQC (version 0.11.5)