FastQCFastQC Report
Mon 4 Feb 2019
000000000-BTF83_l01_n01_Exp2_SrcID47_NL_Cnt5_D4_rep1_SID57.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BTF83_l01_n01_Exp2_SrcID47_NL_Cnt5_D4_rep1_SID57.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences157764
Sequences flagged as poor quality0
Sequence length151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT17381.101645495803859No Hit
AGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTCAATCC13890.8804289952080322No Hit
GTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTA11290.7156258715549808No Hit
GAGCAAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAA10220.6478030475900712No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAA9260.5869526634720215No Hit
AGTCAGTATTCAACAGCTTGTATGCATCTCCACAACTAGAAGGATTTTCA7330.4646180370680256No Hit
ATACCGACTTTGCTAATATTTCAAATCTGTGCTTCAAAAGTGCATTTGGA6430.40757080195735407No Hit
TTTTTAAACTATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG6100.3866534824167744No Hit
ATTATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCA5680.36003143936512766No Hit
CTTAGGGACAACCTTGAACCTGGGACCTTTGATCTTGGGGGGCTATATGA4550.2884054663928399No Hit
GAAGCAGTTTTCTTGATTCAGCTGAAAATCCTTCTAGTTGTGGAGATGCA4540.2877716082249436No Hit
TAGTAGCACTGCCACAACTAACTCAATGCATGTGTAAGGAAGGAGTTGAA4430.2807991683780837No Hit
CACTTGGAAGTATGCTTCATGTATTCAGATTTTCACTTCATCAATGAGCA4280.27129129585963846No Hit
ATATGAAGCAATTGAGGAGTGCCTGATTAATGATCCCTGGGTTTTGCTTA3890.24657082731168073No Hit
GAGCGAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT3720.23579523845744277No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG3720.23579523845744277No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA3590.22755508227479018No Hit
ACCTTGAACCTGGGACCTTTGATCTTGGGGGGCTATATGAAGCAATTGAG3550.22501964960320478No Hit
TTTCAAATCTGTGCTTCAAAAGTGCATTTGGATCACCAAGTTCTACGATT3540.22438579143530843No Hit
GCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTC3490.22121650059582668No Hit
AGCGAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAAG3250.20600390456631426No Hit
GCTTCATATAGCCCCCCAAGATCAAAGGTCCCAGGTTCAAGGTTGTCCCT3230.20473618823052156No Hit
AGCGAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTT3160.20029918105524705No Hit
GTATTCAACAGCTTGTATGCATCTCCACAACTAGAAGGATTTTCAGCTGA3130.198397606551558No Hit
GTCCCTAAGAGCCTGAACGATAAGAAGCAGTTTTCTTGATTCAGCTGAAA2970.1882558758652164No Hit
GTGTAAGGAAGGAGTTGAACCAAGAAGCATTAAGCAAAACCCAGGGATCA2920.18508658502573463No Hit
TCGTAGAACTTGGTGATCCAAATGCACTTTTGAAGCACAGATTTGAAATA2890.18318501052204558No Hit
GAGCGAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAA2830.17938186151466748No Hit
GTATGGGGAGGACCTGAAAATCGAAACAAACAAATTTGCAGCAATATGCA2820.17874800334677113No Hit
TCCTCATTCTGGGCAAAGAAGACAAGAGATATGGGCCAGCACTAAGCATC2770.17557871250728938No Hit
GTAGAAACAAGGTCGTTTTTAAACTATTCGACACTAATTGATGGCCATCC2750.17431099617149667No Hit
GTTCAAGGTTGTCCCTAAGAGCCTGAACGATAAGAAGCAGTTTTCTTGAT2740.17367713800360032No Hit
AGTATGGGGAGGACCTGAAAATCGAAACAAACAAATTTGCAGCAATATGC2720.17240942166780762No Hit
CTTCCAAGTGAGTGCATATTGCTGCAAATTTGTTTGTTTCGATTTTCAGG2680.16987398899622222No Hit
CTCCTCAATTGCTTCATATAGCCCCCCAAGATCAAAGGTCCCAGGTTCAA2650.16797241449253317No Hit
AGTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGT2640.16733855632463682No Hit
ATATAGCCCCCCAAGATCAAAGGTCCCAGGTTCAAGGTTGTCCCTAAGAG2630.16670469815674047No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT2600.16480312365305141No Hit
GAATAAAAGAACTAAGAAATCTAATGTCGCAGTCTCGCACCCGCGAGATA2590.16416926548515506No Hit
GTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACCACGTCTCCTTGC2570.16290154914936233No Hit
GAATGAGGAATCCCCTCAGAACAGCGGACTCCACTCCAGCTGTGCCTTCA2560.16226769098146598No Hit
AGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCAC2550.16163383281356963No Hit
GGTTCAAGGTTGTCCCTAAGAGCCTGAACGATAAGAAGCAGTTTTCTTGA2550.16163383281356963No Hit
ATTGAGGAGTGCCTGATTAATGATCCCTGGGTTTTGCTTAATGCTTCTTG2440.15466139296670978No Hit
GCCACAACTAACTCAATGCATGTGTAAGGAAGGAGTTGAACCAAGAAGCA2430.15402753479881343No Hit
TCATTAATCAGGCACTCCTCAATTGCTTCATATAGCCCCCCAAGATCAAA2420.15339367663091708No Hit
GCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTAAGGAAGGAG2420.15339367663091708No Hit
CTTGGAAGTATGCTTCATGTATTCAGATTTTCACTTCATCAATGAGCAAG2360.14959052762353897No Hit
AAACAAGGTCGTTTTTAAACTATTCGACACTAATTGATGGCCATCCGAAT2250.1426180877766791No Hit
GAGCAAGGCGAGTCAATAATCGTAGAACTTGGTGATCCAAATGCACTTTT2240.14198422960878274No Hit
TCAATGAGCAAGGCGAGTCAATAATCGTAGAACTTGGTGATCCAAATGCA2230.14135037144088639No Hit
AGCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTT2180.13818108060140463No Hit
GTATGCATCTCCACAACTAGAAGGATTTTCAGCTGAATCAAGAAAACTGC2180.13818108060140463No Hit
GAGTATGGGGAGGACCTGAAAATCGAAACAAACAAATTTGCAGCAATATG2170.13754722243350828No Hit
AACTAACTCAATGCATGTGTAAGGAAGGAGTTGAACCAAGAAGCATTAAG2170.13754722243350828No Hit
TCAATGAACTGAGCAACCTTGCGAAAGGAGAGAAGGCTAATTGGGCAAGG2090.13247635709033745No Hit
GCAAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAAGA2040.1293070662508557No Hit
GAGCAAAAGCAGGGTAGATAATCACTCACTGAGTGACATCAAAATCATGG2020.128039349915063No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT2010.12740549174716664No Hit
GGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTAAGGA1950.12360234273978854No Hit
ATCAGGCACTCCTCAATTGCTTCATATAGCCCCCCAAGATCAAAGGTCCC1940.12296848457189219No Hit
GTTCTGAGGGGATTCCTCATTCTGGGCAAAGAAGACAAGAGATATGGGCC1930.12233462640399584No Hit
ATTCAACAGCTTGTATGCATCTCCACAACTAGAAGGATTTTCAGCTGAAT1930.12233462640399584No Hit
GTTCTACGATTATTGACTCGCCTTGCTCATTGATGAAGTGAAAATCTGAA1910.12106691006820314No Hit
GTTCATTGATGCTTAGTGCTGGCCCATATCTCTTGTCTTCTTTGCCCAGA1890.11979919373241044No Hit
ATCAAGAAGTACACATCAGGAAGACAGGAGAAGAACCCAGCACTTAGGAT1890.11979919373241044No Hit
GTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCACT1890.11979919373241044No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT1850.11726376106082503No Hit
GTCGGTATTCAACAGCTTGTATGCATCTCCACAACTAGAAGGATTTTCAG1810.11472832838923962No Hit
GCTTGTATGCATCTCCACAACTAGAAGGATTTTCAGCTGAATCAAGAAAA1780.11282675388555057No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT1770.11219289571765421No Hit
CCTTGAACCTGGGACCTTTGATCTTGGGGGGCTATATGAAGCAATTGAGG1770.11219289571765421No Hit
CAACTAACTCAATGCATGTGTAAGGAAGGAGTTGAACCAAGAAGCATTAA1760.11155903754975786No Hit
ATTATTGACTCGCCTTGCTCATTGATGAAGTGAAAATCTGAATACATGAA1760.11155903754975786No Hit
GTGCTGGCCCATATCTCTTGTCTTCTTTGCCCAGAATGAGGAATCCCCTC1740.11029132121396516No Hit
ATACTGACTTTGCTAATATTTCAAATCTGTGCTTCAAAAGTGCATTTGGA1690.10712203037448341No Hit
GGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATG1680.10648817220658706No Hit
GATTTGAAATATTAGCAAAGTCGGTATTCAACAGCTTGTATGCATCTCCA1670.10585431403869071No Hit
GTCAGTATTCAACAGCTTGTATGCATCTCCACAACTAGAAGGATTTTCAG1670.10585431403869071No Hit
AGCAAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAAG1660.10522045587079436No Hit
ATCTAATGTCGCAGTCTCGCACCCGCGAGATACTCACAAAAACCACCGTG1660.10522045587079436No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA1610.1020511650313126No Hit
GATCAAAGGTCCCAGGTTCAAGGTTGTCCCTAAGAGCCTGAACGATAAGA1600.10141730686341623No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTT1590.10078344869551988No Hit
GAAGTATGCTTCATGTATTCAGATTTTCACTTCATCAATGAGCAAGGCGA1580.10014959052762352No Hit
GCTTAGTGCTGGCCCATATCTCTTGTCTTCTTTGCCCAGAATGAGGAATC1580.10014959052762352No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1580.10014959052762352No Hit
TGTGTAAGGAAGGAGTTGAACCAAGAAGCATTAAGCAAAACCCAGGGATC1580.10014959052762352No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCTTA100.007085243145.0145
AAGCCCG100.007085243145.0145
TCCCGAT100.007085243145.0145
TGGCTCA100.007085243145.0145
TCGGTCG100.007085243145.0145
CGAAGCG100.007085243145.0145
GAGCGAA800.0135.93751
AGGTAGT256.4478754E-6116.06
GGTAGTT256.4478754E-6116.07
GAGCAAA5950.098.697481
GTAGTTT301.595266E-596.6666648
GCAAAAG6100.096.2704853
TCTCCGC259.371993E-487.0145
CAAAAGC7400.079.358114
CGAAAGC1900.076.315793
AGCGAAA1900.076.315791
AAGCAGG9150.076.065587
GCAGGTC2700.075.185199
GCAGGGT1350.075.185199
AGCCCCG300.00193271172.5145
Produced by FastQC (version 0.11.5)