FastQCFastQC Report
Mon 4 Feb 2019
000000000-BTF83_l01_n01_Exp2_SrcID43_NL_Cnt1_D4_rep2_SID70.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BTF83_l01_n01_Exp2_SrcID43_NL_Cnt1_D4_rep2_SID70.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences119995
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTCAATCC10110.8425351056294013No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT9990.8325346889453726No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTT7240.6033584732697196No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAA7100.5916913204716864No Hit
GTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTA7020.5850243760156674No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATTCACTATTTT4250.3541814242260094No Hit
CTTAGGGACAACCTTGAACCTGGGACCTTTGATCTTGGGGGGCTATATGA3750.31251302137589065No Hit
TAGTAGCACTGCCACAACTAACTCAATGCATGTGTAAGGAAGGAGTTGAA3570.2975123963498479No Hit
GGATATGGTTTCCAGAGCCCGAATTGATGCACGGATTGATTTCGAATCTG3110.25917746572773864No Hit
CCATATCCCGACTGGTCTTCTGTATGAACTGCTGGGGAAGAATTTTTCAA3110.25917746572773864No Hit
ATATGAAGCAATTGAGGAGTGCCTGATTAATGATCCCTGGGTTTTGCTTA3090.2575107296137339No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG2900.24167673653068877No Hit
ACCTTGAACCTGGGACCTTTGATCTTGGGGGGCTATATGAAGCAATTGAG2800.233343055960665No Hit
CCATAGCCTCCACCATACTGGATATCCCGACTGGTCTTCTGTATGAACTG2500.2083420142505938No Hit
GGATATCCAGTATGGTGGAGGCTATGGTTTCCAGAGCCCGAATTGATGCA2460.20500854202258428No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGTGAACTCTTCTTTCTTT2390.19917496562356765No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA2380.19834159756656525No Hit
GCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTC2230.18584107671152963No Hit
ATTGAGGAGTGCCTGATTAATGATCCCTGGGTTTTGCTTAATGCTTCTTG2200.18334097254052253No Hit
AGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCAC2150.17917413225551065No Hit
GCTTCATATAGCCCCCCAAGATCAAAGGTCCCAGGTTCAAGGTTGTCCCT2150.17917413225551065No Hit
CTCCTCAATTGCTTCATATAGCCCCCCAAGATCAAAGGTCCCAGGTTCAA2070.17250718779949165No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT2070.17250718779949165No Hit
TCATTAATCAGGCACTCCTCAATTGCTTCATATAGCCCCCCAAGATCAAA2050.1708404516854869No Hit
GAGCGAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT2010.1675069794574774No Hit
AACTAACTCAATGCATGTGTAAGGAAGGAGTTGAACCAAGAAGCATTAAG2000.166673611400475No Hit
GTGTAAGGAAGGAGTTGAACCAAGAAGCATTAAGCAAAACCCAGGGATCA1990.16584024334347264No Hit
AGCGAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTT1940.16167340305846076No Hit
GCCACAACTAACTCAATGCATGTGTAAGGAAGGAGTTGAACCAAGAAGCA1930.1608400350014584No Hit
TTCTTTATCCTTCCAGATTCGAAATCAATCCGTGCATCAATTCGGGCTCT1920.160006666944456No Hit
AAACAAGGGTGTTTTTTATTATTAAATAAGCTGAAACGAGAAAGTTCTTA1920.160006666944456No Hit
GCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTAAGGAAGGAG1890.1575065627734489No Hit
ATATAGCCCCCCAAGATCAAAGGTCCCAGGTTCAAGGTTGTCCCTAAGAG1880.15667319471644653No Hit
GTGTTATCATTCCATTCAAGTCCCCCGATGAGGACTCCAACTGCATTTTT1640.13667236134838953No Hit
GCATTAAGCAAAACCCAGGGATCATTAATCAGGCACTCCTCAATTGCTTC1590.13250552106337765No Hit
GTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCACT1550.12917204883536815No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGCTAAATTCACTATTTTTGCCG1530.1275053127213634No Hit
GTATCGTTCAGGCTCTTAGGGACAACCTTGAACCTGGGACCTTTGATCTT1530.1275053127213634No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT1530.1275053127213634No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC1520.12667194466436102No Hit
CAACTAACTCAATGCATGTGTAAGGAAGGAGTTGAACCAAGAAGCATTAA1510.12583857660735864No Hit
CTCTAATATTGCTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAA1510.12583857660735864No Hit
GGACAAGCTAAATTCACTATTTTTGCCGTCTGAGCTCTTCAATGGTGGAA1500.12500520855035627No Hit
GTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTCAATCCGATGA1450.12083836826534439No Hit
GCTAAATTCACTATTTTTGCCGTCTGAGCTCTTCAATGGTGGAACAGATC1420.11833826409433726No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1420.11833826409433726No Hit
GTATGGGGAGGACCTGAAAATCGAAACAAACAAATTTGCAGCAATATGCA1410.1175048960373349No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA1410.1175048960373349No Hit
CCTTGAACCTGGGACCTTTGATCTTGGGGGGCTATATGAAGCAATTGAGG1400.1166715279803325No Hit
GAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTT1370.11417142380932539No Hit
CCTCAATTGCTTCATATAGCCCCCCAAGATCAAAGGTCCCAGGTTCAAGG1360.113338055752323No Hit
AGTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGT1340.11167131963831826No Hit
CTCCAGCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCACTCT1330.11083795158131589No Hit
CTTCTTTCTTTATCCTTCCAGATTCGAAATCAATCCGTGCATCAATTCGG1320.11000458352431351No Hit
TGTGTAAGGAAGGAGTTGAACCAAGAAGCATTAAGCAAAACCCAGGGATC1240.10333763906829452No Hit
ATATACAACAGGATGGGGGCTGTGACCACTGAAGTGGCATTTGGCCTGGT1230.10250427101129214No Hit
ACCATATCCCGACTGGTCTTCTGTATGAACTGCTGGGGAAGAATTTTTCA1220.10167090295428977No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA351.0913936E-11145.01
TGTTAAC100.007083141145.0145
AGCCCCG151.2273295E-4144.99998145
GCTACGT151.2273295E-4144.99998145
CTGCTTA256.4415235E-6116.0145
AGCAGGC900.0112.777788
TGCCGTT553.6379788E-12105.45455145
AGTAGAA1800.092.6388851
TCTCCGC259.3664764E-487.0145
GCAAAAG3750.086.999993
GCAGGCA1200.084.583339
AGCGAAA950.076.315791
CAAAAGC4350.075.000014
AGCCCAG502.2938711E-672.5145
CACGAGC300.001931577772.49999145
CGAGACC300.001931577772.49999145
AAGCAGG5400.071.157417
GAGCAAA4650.070.161291
CGAAAGC1050.069.0476153
AGAAACA2450.068.0612264
Produced by FastQC (version 0.11.5)