FastQCFastQC Report
Mon 4 Feb 2019
000000000-BTF83_l01_n01_Exp2_SrcID43_NL_Cnt1_D4_rep1_SID53.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BTF83_l01_n01_Exp2_SrcID43_NL_Cnt1_D4_rep1_SID53.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences188301
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTCAATCC16190.8597936282866262No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT15960.8475791419057785No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTT11240.5969166387857738No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAA10680.567177019771536No Hit
GTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTA10100.5363752715067897No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATTCACTATTTT6360.3377571016617012No Hit
GGATATGGTTTCCAGAGCCCGAATTGATGCACGGATTGATTTCGAATCTG5180.2750914758817No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG4680.24853824461898766No Hit
TAGTAGCACTGCCACAACTAACTCAATGCATGTGTAAGGAAGGAGTTGAA4650.24694505074322495No Hit
CTTAGGGACAACCTTGAACCTGGGACCTTTGATCTTGGGGGGCTATATGA4460.23685482286339424No Hit
ATATGAAGCAATTGAGGAGTGCCTGATTAATGATCCCTGGGTTTTGCTTA4210.22357820723203806No Hit
CCATAGCCTCCACCATACTGGATATCCCGACTGGTCTTCTGTATGAACTG4120.21879862560474986No Hit
ACCTTGAACCTGGGACCTTTGATCTTGGGGGGCTATATGAAGCAATTGAG4050.2150811732279701No Hit
GCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTC4000.21242585010169887No Hit
GGATATCCAGTATGGTGGAGGCTATGGTTTCCAGAGCCCGAATTGATGCA3960.21030159160068188No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA3930.20870839772491911No Hit
CCATATCCCGACTGGTCTTCTGTATGAACTGCTGGGGAAGAATTTTTCAA3840.20392881609763094No Hit
AGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCAC3510.18640368346424077No Hit
GCTTCATATAGCCCCCCAAGATCAAAGGTCCCAGGTTCAAGGTTGTCCCT3250.17259600320763033No Hit
AGCGAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTT3220.1710028093318676No Hit
AAACAAGGGTGTTTTTTATTATTAAATAAGCTGAAACGAGAAAGTTCTTA3160.1678164215803421No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT3070.1630368399530539No Hit
GAGCGAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT3030.1609125814520369No Hit
CTCCTCAATTGCTTCATATAGCCCCCCAAGATCAAAGGTCCCAGGTTCAA3020.16038151682678264No Hit
GCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTAAGGAAGGAG3010.1598504522015284No Hit
GTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCACT2930.15560193519949445No Hit
GCCACAACTAACTCAATGCATGTGTAAGGAAGGAGTTGAACCAAGAAGCA2900.15400874132373168No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT2900.15400874132373168No Hit
ATTGAGGAGTGCCTGATTAATGATCCCTGGGTTTTGCTTAATGCTTCTTG2870.15241554744796895No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGTGAACTCTTCTTTCTTT2720.14444957806915523No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2700.14338744881864676No Hit
TTCTTTATCCTTCCAGATTCGAAATCAATCCGTGCATCAATTCGGGCTCT2690.1428563841933925No Hit
TCATTAATCAGGCACTCCTCAATTGCTTCATATAGCCCCCCAAGATCAAA2640.14020106106712127No Hit
CTCTAATATTGCTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAA2580.13701467331559578No Hit
AACTAACTCAATGCATGTGTAAGGAAGGAGTTGAACCAAGAAGCATTAAG2540.13489041481457878No Hit
GTGTAAGGAAGGAGTTGAACCAAGAAGCATTAAGCAAAACCCAGGGATCA2470.13117296243779905No Hit
GTATGGGGAGGACCTGAAAATCGAAACAAACAAATTTGCAGCAATATGCA2440.12957976856203632No Hit
GCATTAAGCAAAACCCAGGGATCATTAATCAGGCACTCCTCAATTGCTTC2410.12798657468627359No Hit
GGACAAGCTAAATTCACTATTTTTGCCGTCTGAGCTCTTCAATGGTGGAA2370.1258623161852566No Hit
ATATAGCCCCCCAAGATCAAAGGTCCCAGGTTCAAGGTTGTCCCTAAGAG2360.12533125156000233No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC2330.12373805768423958No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA2310.12267592843373111No Hit
GTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTCAATCCGATGA2270.12055166993271411No Hit
GTGTTATCATTCCATTCAAGTCCCCCGATGAGGACTCCAACTGCATTTTT2270.12055166993271411No Hit
CAACTAACTCAATGCATGTGTAAGGAAGGAGTTGAACCAAGAAGCATTAA2260.12002060530745987No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGCTAAATTCACTATTTTTGCCG2210.11736528218118862No Hit
GTATCGTTCAGGCTCTTAGGGACAACCTTGAACCTGGGACCTTTGATCTT2200.11683421755593439No Hit
TGATAACACAGTTCGAGTCTCTGAAACTCTACAGAGATTCGCTTGGAGAA2160.11470995905491739No Hit
ATCAGGCACTCCTCAATTGCTTCATATAGCCCCCCAAGATCAAAGGTCCC2140.11364782980440889No Hit
AGTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGT2100.1115235713033919No Hit
GAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTT2080.1104614420528834No Hit
ATATACAACAGGATGGGGGCTGTGACCACTGAAGTGGCATTTGGCCTGGT2030.10780611892661218No Hit
CTTCTTTCTTTATCCTTCCAGATTCGAAATCAATCCGTGCATCAATTCGG2000.10621292505084944No Hit
GCTAAATTCACTATTTTTGCCGTCTGAGCTCTTCAATGGTGGAACAGATC1930.10249547267406972No Hit
ATGCATGTGTAAGGAAGGAGTTGAACCAAGAAGCATTAAGCAAAACCCAG1930.10249547267406972No Hit
AGCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTT1900.10090227879830697No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCTAG100.0070863245145.0145
GAGCGAA700.0145.01
GCAGGAG100.0070863245145.09
AGCAGGC2250.0112.777788
CTGTCCT203.8611316E-4108.75145
CTGCTTA553.6379788E-12105.454544145
GCAAAAG7050.099.751773
GAGCAAA7150.098.3566361
TCGTATC301.5960735E-596.666664145
TTTCCTA406.098089E-790.625145
CAAAAGC7900.089.018994
TGCCCTA259.374834E-487.0145
TATATTC259.374834E-487.03
AAGCAGG9600.084.5833367
GCAGGCA3150.080.555569
GCAGGGT2350.077.127669
TTATATT300.001933294972.52
ATTATAT300.001933294972.51
AGTAGAA2700.072.51
CTGTCTT1150.069.347824145
Produced by FastQC (version 0.11.5)