FastQCFastQC Report
Mon 4 Feb 2019
000000000-BTF83_l01_n01_Exp2_SrcID41_NL_Ind1_D4_rep2_SID68.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BTF83_l01_n01_Exp2_SrcID41_NL_Ind1_D4_rep2_SID68.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72320
Sequences flagged as poor quality0
Sequence length151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT11511.591537610619469No Hit
AGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTCAATCC9801.3550884955752212No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTT8631.1933075221238938No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAA6790.9388827433628318No Hit
GTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTA6390.8835730088495576No Hit
GAGCAAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAA6150.8503871681415929No Hit
TTTTTAAACTATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG3990.5517146017699115No Hit
ATTATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCA3680.5088495575221239No Hit
TAGTAGCACTGCCACAACTAACTCAATGCATGTGTAAGGAAGGAGTTGAA2970.4106747787610619No Hit
ATATGAAGCAATTGAGGAGTGCCTGATTAATGATCCCTGGGTTTTGCTTA2720.37610619469026546No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATTCACTATTTT2600.35951327433628316No Hit
CTTAGGGACAACCTTGAACCTGGGACCTTTGATCTTGGGGGGCTATATGA2530.3498340707964602No Hit
GCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTC2520.34845132743362833No Hit
ACCTTGAACCTGGGACCTTTGATCTTGGGGGGCTATATGAAGCAATTGAG2400.33185840707964603No Hit
CCCTTATACTGGAGACCCTCCTTACAGCCATGGGACAGGAACAGGATACA2310.31941371681415925No Hit
GAGCGAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT2220.3069690265486726No Hit
ATTGAGGAGTGCCTGATTAATGATCCCTGGGTTTTGCTTAATGCTTCTTG2220.3069690265486726No Hit
ATACTGGATATCCCGACTGGTCTTCTGTATGAACTGCTGGGGAAGAATTT2160.29867256637168144No Hit
CAATTAGCCTTCTCTCCTTTCGCAAGGTTGCTCAGTTCATTGATGCTTAG2150.29728982300884954No Hit
GTATGGGGAGGACCTGAAAATCGAAACAAACAAATTTGCAGCAATATGCA2050.283462389380531No Hit
CCATGTCAACAGGACACATCAGTACTCAGAAAAGGGAAGATGGACAACAA2050.283462389380531No Hit
GTTCAAGGTTGTCCCTAAGAGCCTGAACGATAAGAAGCAGTTTTCTTGAT2020.2793141592920354No Hit
GTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACCACGTCTCCTTGC1980.273783185840708No Hit
CTCCTCAATTGCTTCATATAGCCCCCCAAGATCAAAGGTCCCAGGTTCAA1960.27101769911504425No Hit
AGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCAC1940.2682522123893806No Hit
GATCTTCATGATCTCAGTGAACTCTTCTTTCTTTATCCTTCCAGATTCGA1930.2668694690265487No Hit
GCTTCATATAGCCCCCCAAGATCAAAGGTCCCAGGTTCAAGGTTGTCCCT1920.2654867256637168No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT1900.2627212389380531No Hit
CTTCCAAGTGAGTGCATATTGCTGCAAATTTGTTTGTTTCGATTTTCAGG1880.25995575221238937No Hit
TCATTAATCAGGCACTCCTCAATTGCTTCATATAGCCCCCCAAGATCAAA1860.2571902654867257No Hit
GGATATCCCGACTGGTCTTCTGTATGAACTGCTGGGGAAGAATTTTTCAA1850.2558075221238938No Hit
GAAGCAGTTTTCTTGATTCAGCTGAAAATCCTTCTAGTTGTGGAGATGCA1840.25442477876106195No Hit
CCATGGAACAAATGTACCAAAGGTGCTGCAATTTATTTGAAAAATTCTTC1830.25304203539823006No Hit
AGCGAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTT1820.2516592920353983No Hit
GCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTAAGGAAGGAG1800.2488938053097345No Hit
GCCACAACTAACTCAATGCATGTGTAAGGAAGGAGTTGAACCAAGAAGCA1760.24336283185840707No Hit
GTGTAAGGAAGGAGTTGAACCAAGAAGCATTAAGCAAAACCCAGGGATCA1750.2419800884955752No Hit
ATACTGGAGACCCTCCTTACAGCCATGGGACAGGAACAGGATACACCATG1710.23644911504424776No Hit
GGCTAATTGGGCAAGGAGACGTGGTGTTGGTAATGAAACGGAAACGGGAC1680.2323008849557522No Hit
GAATAAAAGAACTAAGAAATCTAATGTCGCAGTCTCGCACCCGCGAGATA1680.2323008849557522No Hit
ATATAGCCCCCCAAGATCAAAGGTCCCAGGTTCAAGGTTGTCCCTAAGAG1670.23091814159292035No Hit
GTCCCTAAGAGCCTGAACGATAAGAAGCAGTTTTCTTGATTCAGCTGAAA1560.2157079646017699No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGTGAACTCTTCTTTCTTT1560.2157079646017699No Hit
GGTTCAAGGTTGTCCCTAAGAGCCTGAACGATAAGAAGCAGTTTTCTTGA1540.2129424778761062No Hit
TTCTTTATCCTTCCAGATTCGAAATCAATCCGTGCATCAATTCGGGCTCT1540.2129424778761062No Hit
GTTCATCTTCAAGTACTCGGTGTTTGTTGTCCATCTTCCCTTTTCTGAGT1530.21155973451327434No Hit
AACTAACTCAATGCATGTGTAAGGAAGGAGTTGAACCAAGAAGCATTAAG1530.21155973451327434No Hit
GCAGAAGGATTTTCAGCTGAATCAAGAAAACTGCTTCTTATCGTTCAGGC1500.2074115044247788No Hit
AGTATGGGGAGGACCTGAAAATCGAAACAAACAAATTTGCAGCAATATGC1500.2074115044247788No Hit
GTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCACT1470.20326327433628316No Hit
GAGCGAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAA1440.19911504424778761No Hit
GTAGAAACAAGGTCGTTTTTAAACTATTCGACACTAATTGATGGCCATCC1440.19911504424778761No Hit
GAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTT1430.19773230088495575No Hit
CAATTAGCACATTAGCCTTCTCTCCTTTCGCAAGGTTGCTCAGTTCATTG1420.1963495575221239No Hit
AGCGAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAAG1410.19496681415929204No Hit
AAACAAGGTCGTTTTTAAACTATTCGACACTAATTGATGGCCATCCGAAT1380.19081858407079647No Hit
GGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTAAGGA1360.18805309734513273No Hit
GATCTTGAACTCTTCTTTCTTTATCCTTCCAGATTCGAAATCAATCCGTG1360.18805309734513273No Hit
AGCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTT1330.18390486725663718No Hit
GGCTAATGTGCTAATTGGGCAAGGAGACGTGGTGTTGGTAATGAAACGGA1290.17837389380530974No Hit
GCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTTTC1290.17837389380530974No Hit
TAAAAGCCAGCACAAAATGCTATAAGCACAACTTTCCCTTATACTGGAGA1280.17699115044247787No Hit
GTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTCAATCCGATGA1260.17422566371681417No Hit
CCTCAATTGCTTCATATAGCCCCCCAAGATCAAAGGTCCCAGGTTCAAGG1250.1728429203539823No Hit
AGTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGT1240.17146017699115043No Hit
ATCAGGCACTCCTCAATTGCTTCATATAGCCCCCCAAGATCAAAGGTCCC1230.1700774336283186No Hit
CCTTGAACCTGGGACCTTTGATCTTGGGGGGCTATATGAAGCAATTGAGG1230.1700774336283186No Hit
AGCAAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAAG1180.16316371681415928No Hit
ATCCAGTATGGTGGAGGCTATGGTTTCCAGAGCCCGAATTGATGCACGGA1160.16039823008849557No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT1150.15901548672566373No Hit
GTTGACAGTATCCATGGTGTATCCTGTTCCTGTCCCATGGCTGTAAGGAG1150.15901548672566373No Hit
GCAAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAAGA1150.15901548672566373No Hit
GCATTAAGCAAAACCCAGGGATCATTAATCAGGCACTCCTCAATTGCTTC1150.15901548672566373No Hit
TCAATGAACTGAGCAACCTTGCGAAAGGAGAGAAGGCTAATGTGCTAATT1140.15763274336283187No Hit
CAACTAACTCAATGCATGTGTAAGGAAGGAGTTGAACCAAGAAGCATTAA1110.1534845132743363No Hit
GGCCAGCACTAAGCATCAATGAACTGAGCAACCTTGCGAAAGGAGAGAAG1110.1534845132743363No Hit
CCATGGATACTGTCAACAGGACACATCAGTACTCAGAAAAGGGAAGATGG1090.15071902654867259No Hit
CTCAATGCATGTGTAAGGAAGGAGTTGAACCAAGAAGCATTAAGCAAAAC1090.15071902654867259No Hit
GGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATG1080.14933628318584072No Hit
ATATGGCCATAATCAAGAAGTACACATCAGGAAGACAGGAGAAGAACCCA1080.14933628318584072No Hit
ATCTAATGTCGCAGTCTCGCACCCGCGAGATACTCACAAAAACCACCGTG1070.14795353982300885No Hit
GGACAAGCTAAATTCACTATTTTTGCCGTCTGAGCTCTTCAATGGTGGAA1070.14795353982300885No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG1050.14518805309734514No Hit
GCTAAATTCACTATTTTTGCCGTCTGAGCTCTTCAATGGTGGAACAGATC1040.14380530973451328No Hit
TGTGTAAGGAAGGAGTTGAACCAAGAAGCATTAAGCAAAACCCAGGGATC1040.14380530973451328No Hit
ACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG1030.14242256637168144No Hit
ATCAAGAAGTACACATCAGGAAGACAGGAGAAGAACCCAGCACTTAGGAT1030.14242256637168144No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACCACGT1020.14103982300884957No Hit
GCATACTTCCAAGTGAGTGCATATTGCTGCAAATTTGTTTGTTTCGATTT1010.1396570796460177No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGCTAAATTCACTATTTTTGCCG990.136891592920354No Hit
ACCCAGGGATCATTAATCAGGCACTCCTCAATTGCTTCATATAGCCCCCC980.13550884955752213No Hit
GCTTGTATGCATCTCCACAACTAGAAGGATTTTCAGCTGAATCAAGAAAA970.1341261061946903No Hit
CACTTGGAAGTATGCTTCATGTATTCAGATTTTCACTTCATCAATGAGCA960.1327433628318584No Hit
GAGTATGGGGAGGACCTGAAAATCGAAACAAACAAATTTGCAGCAATATG960.1327433628318584No Hit
GCAATATGCACTCACTTGGAAGTATGCTTCATGTATTCAGATTTTCACTT950.13136061946902655No Hit
TCCCCATACTCTTTCATTGTTTTTTCCGCAAGCTCGACAATCATCGGATT940.12997787610619468No Hit
CTTCTTTCTTTATCCTTCCAGATTCGAAATCAATCCGTGCATCAATTCGG930.12859513274336284No Hit
GGATACACCATGGATACTGTCAACAGGACACATCAGTACTCAGAAAAGGG920.12721238938053098No Hit
CAGTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTG920.12721238938053098No Hit
GCTTCTTATCGTTCAGGCTCTTAGGGACAACCTTGAACCTGGGACCTTTG920.12721238938053098No Hit
GCAATATGCACTCACTTGGAAGTATGCTTCATGTAACTAGAAGGATTTTC910.12582964601769914No Hit
ATACCGACTTTGCTAATATTTCAAATCTGTGCTTCAAAAGTGCATTTGGA910.12582964601769914No Hit
AGTAGAAACAAGGTCGTTTTTAAACTATTCGACACTAATTGATGGCCATC900.12444690265486726No Hit
GATCAAAGGTCCCAGGTTCAAGGTTGTCCCTAAGAGCCTGAACGATAAGA900.12444690265486726No Hit
CTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAA880.12168141592920353No Hit
ATCCTGTTCCTGTCCCATGGCTGTAAGGAGGGTCTCCAGTATAAGGGAAA870.1202986725663717No Hit
TCCCTGGGTTTTGCTTAATGCTTCTTGGTTCAACTCCTTCCTTACACATG870.1202986725663717No Hit
AACTCAATGCATGTGTAAGGAAGGAGTTGAACCAAGAAGCATTAAGCAAA860.11891592920353983No Hit
ATGCATGTGTAAGGAAGGAGTTGAACCAAGAAGCATTAAGCAAAACCCAG860.11891592920353983No Hit
CCTTACAGCCATGGGACAGGAACAGGATACACCATGGATACTGTCAACAG850.11753318584070797No Hit
ATCTTCATGATCTCAGTGAACTCTTCTTTCTTTATCCTTCCAGATTCGAA840.1161504424778761No Hit
GGAACTTTCCTCCACTCCTTTGGGGGACTCTCCAATGGGCCATGTTTCTG830.11476769911504425No Hit
ATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCAGTC830.11476769911504425No Hit
GCTTCAATCCGATGATTGTCGAGCTTGCGGAAAAAACAATGAAAGAGTAT830.11476769911504425No Hit
GTGCTGGCCCATATCTCTTGTCTTCTTTGTCTGCTGTAATTGGATATTTC830.11476769911504425No Hit
GGTGTATCCTGTTCCTGTCCCATGGCTGTAAGGAGGGTCTCCAGTATAAG830.11476769911504425No Hit
GATTAATGATCCCTGGGTTTTGCTTAATGCTTCTTGGTTCAACTCCTTCC810.11200221238938055No Hit
TCTTTATCCTTCCAGATTCGAAATCAATCCGTGCATCAATTCGGGCTCTG810.11200221238938055No Hit
GAAAAATGAAAGAGTATGGGGAGGACCTGAAAATCGAAACAAACAAATTT800.11061946902654868No Hit
TCTTCATGATCTCAGTGAACTCTTCTTTCTTTATCCTTCCAGATTCGAAA800.11061946902654868No Hit
AAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCACTGCCA790.10923672566371682No Hit
CCTTCTGCTGCAAATTTGTTTGTTTCGATTTTCAGGTCCTCCCCATACTC780.10785398230088496No Hit
CCACCATACTGGATATCCCGACTGGTCTTCTGTATGAACTGCTGGGGAAG780.10785398230088496No Hit
GTTCCATGGTGTATCCTGTTCCTGTCCCATGGCTGTAAGGAGGGTCTCCA770.1064712389380531No Hit
GTATGAACTGCTGGGGAAGAATTTTTCAAATAAATTGCAGCACCTTTGGT770.1064712389380531No Hit
AGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTTT750.1037057522123894No Hit
CAATTGAGGAGTGCCTGATTAATGATCCCTGGGTTTTGCTTAATGCTTCT740.10232300884955751No Hit
GTATGGTGGAGGCTATGGTTTCCAGAGCCCGAATTGATGCACGGATTGAT730.10094026548672567No Hit
CCCCATACTCTTTCATTGTTTTTTCCGCAAGCTCGACAATCATCGGATTG730.10094026548672567No Hit
AGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTTT730.10094026548672567No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTCGT100.0070773587145.00002145
AGGGTGT100.0070773587145.000026
GTATATA100.0070773587145.000024
TATAGGT100.0070773587145.000027
AAGGGTG100.0070773587145.000025
GGCTACC100.0070773587145.00002145
GGATCCG151.2253276E-4145.0145
GCATTTC151.2253276E-4145.0145
GAGCGAA700.0134.642851
GAGCAAA2800.0126.8751
AGTAGAA850.0119.411761
TTGCTCG256.4240758E-6116.0145
TAGAAAC950.0106.842113
AGCAGGC1850.0101.8918848
GTAGAAA1000.0101.52
GCAAAAG3750.094.733343
GCAGGCA2000.094.259
CAAAAGC3800.093.486854
GCAGGTC950.091.578959
TCGTATC406.0598904E-790.62501145
Produced by FastQC (version 0.11.5)