FastQCFastQC Report
Mon 4 Feb 2019
000000000-BTF83_l01_n01_Exp2_SrcID37_Shed_Cnt2_D4_rep1_SID50.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BTF83_l01_n01_Exp2_SrcID37_Shed_Cnt2_D4_rep1_SID50.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences169171
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA5980.35348848206843964No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG5930.35053289275348615No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT4480.2648208026198344No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA4440.2624563311678716No Hit
ATATACAACAGGATGGGGGCTGTGACCACTGAAGTGGCATTTGGCCTGGT3420.20216230914282No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT3330.19684224837590367No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3200.18915771615702454No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGATCCCCGTTCCC2990.17674424103421982No Hit
AAACAAGGGTGTTTTTTATTATTAAATAAGCTGAAACGAGAAAGTTCTTA2880.1702419445413221No Hit
GTATACATCATGGTCATAAGTCCCATTTCTGATTGACTCTATGCAAGCGT2850.16846859095235No Hit
GAGCAAAAGCAGGGGATAATTCTATTAATCATGAAGACCATCATTGCTTT2570.15191729078861033No Hit
CTCTAATATTGCTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAA2490.14718834788468474No Hit
AGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTCAATCC2340.1383215799398242No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT2340.1383215799398242No Hit
TCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGATCCCCGTTCCCA2330.13773046207683348No Hit
TCCTAGCTCCAGTGCTGGTCTGAAAAATGATCTTCTTGAAAATTTGCAGG2300.13595710848786138No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC2270.1341837548988893No Hit
CTACTAGACAGACCCCTTACCCAGGGTCTGGACCCGATATTCGGGATTAT2220.1312281655839358No Hit
GTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGATGTT2220.1312281655839358No Hit
GATGTATACAGAGACGAAGCATTAAACAACCGGTTTCAGATCAAAGGTGT2180.12886369413197296No Hit
TGATAACACAGTTCGAGTCTCTGAAACTCTACAGAGATTCGCTTGGAGAA2140.12649922268001015No Hit
CTCCAGCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCACTCT2130.12590810481701947No Hit
GTGTTATCATTCCATTCAAGTCCCCCGATGAGGACTCCAACTGCATTTTT2040.12058804405010316No Hit
GAGCAAAAGCAGGGTAGATAATCACTCACTGAGTGACATCAAAATCATGG2040.12058804405010316No Hit
GATGTAAGGCTTGCATAAATGTTATTTGCTCAAAACTATTCTCTGTTATC1960.11585910114617753No Hit
GTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTA1950.11526798328318684No Hit
GTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGAGTG1850.10935680465327981No Hit
GGTATGTGCAACCTGTGAACAGATTGCTGACTCCCAGCATCGGTCTCATA1820.10758345106430772No Hit
GGCTAAAACCATTCTGTTCTCATGTCTGATTAGTGGATTGGTTGTTGTCA1780.10521897961234492No Hit
CTCTCTATCATCCCGTCAGGCCCCCTCAAAGCCGAGATCGCACAGAGACT1770.1046278617493542No Hit
ATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTATGA1760.1040367438863635No Hit
CCTGTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGAT1760.1040367438863635No Hit
GGTCCATGCTCATACCCAAGCAGAAAGTGGCAGGCCCTCTTTGTATCAGA1710.10108115457141No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGCC100.007085692145.0145
GGCCGGC100.007085692145.0145
CTCTACT151.2282134E-4145.0145
AGCAGGC256.449234E-6116.08
CTGCTTA352.751749E-7103.57143145
GAGCGAA451.0861186E-896.6666641
GCAGGAG301.5956013E-596.6666649
TACAACG259.373174E-487.0145
GCAAAAG4950.083.4848563
GCAGGGT2150.077.5581369
GAGCAAA5600.075.089291
CAAAAGC5550.074.459464
AGCCCAG809.822543E-1172.5145
TCTTAAT406.647207E-572.52
TCGTATC406.647207E-572.5145
TTCTCCG300.001932953472.5145
AAGCAGG6350.071.929147
AGCGAAA1150.069.347831
GGGGCCG451.19100354E-464.44444145
AGCAGGG3000.062.8333368
Produced by FastQC (version 0.11.5)