FastQCFastQC Report
Mon 4 Feb 2019
000000000-BTF83_l01_n01_Exp2_SrcID35_Shed_Ind2_D4_rep2_SID66.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BTF83_l01_n01_Exp2_SrcID35_Shed_Ind2_D4_rep2_SID66.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences125799
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGTATCCTTCCAGATTCGAAATCAATCCGTGCATCAATTCGGGCTCTGG19971.5874530004213068No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTT16911.3442078235916024No Hit
CAGTGAACTCTTCTTTCTTTATCCTTCCAGATTCGAAATCAATCCGTGCA12821.019086002273468No Hit
GGAACATGGTTTCCAGAGCCCGAATTGATGCACGGATTGATTTCGAATCT11150.8863345495592175No Hit
GGAACAGGATACACCATGGATACCAGTCGGGATATCCAGTATGGTGGAGG10830.8608971454463071No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT9280.737684719274398No Hit
AGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTCAATCC7880.6263960762804156No Hit
CTCAGACTGGATATCCCGACTGGTCTTCTGTTGAACTCTTCTTTCTTTAT7490.5953942400178062No Hit
GTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTA5950.4729767327244254No Hit
CAGACTGGATATCCCGACTGGTCTTCTGTTGAACTCTTCTTTCTTTATCC5730.4554885173967997No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAA5160.4101781413206782No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATTCACTATTTT5030.3998441958998084No Hit
CTCAGTGAACTCTTCTTTCTTTATCCTTCCAGATTCGAAATCAATCCGTG4360.34658463103840254No Hit
TCTCAGTGAACTCTTCTTTCTTTATCCTTCCAGATTCGAAATCAATCCGT4130.3283014968322483No Hit
CCATAGCCTCCACCATACTGGATATCCCGACTGGTATCCATGGTGTATCC3470.2758368508493708No Hit
CCATGTTCCTGTCCCATGGCTGTAAGGAGGGTCTCCAGTATAAGGGAAAG2910.23132139365177784No Hit
GATGGACAAATGTACCAAAGGTGCTGCAATTTATTTGAAAAATTCTTCCC2680.21303825944562357No Hit
CACTTGGAAGTATGCTTCATGTATTCAGATTTTCACTTCATCAATGAGCA2640.2098585839315098No Hit
TAGTAGCACTGCCACAACTAACTCAATGCATGTGTAAGGAAGGAGTTGAA2630.20906366505298135No Hit
CTTTTCTTACTGGAGACCCTCCTTACAGCCATGGGACAGGAACAGGATAC2550.20270431402475375No Hit
ATATGAAGCAATTGAGGAGTGCCTGATTAATGATCCCTGGGTTTTGCTTA2450.1947551252394693No Hit
TCTCAGACTGGATATCCCGACTGGTCTTCTGTTGAACTCTTCTTTCTTTA2400.19078053084682708No Hit
GCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTTTC2360.1876008553327133No Hit
GAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTT2350.18680593645418486No Hit
CCATAGCCTCCACCATACTGGATATCCCGACTGGTCTTCTGTATGAACTG2300.1828313420615426No Hit
CCATGGATACCAGTCGGGATATCCAGTATGGTGGAGGCTATGGTTTCCAG2280.18124150430448574No Hit
GGACAAGCTAAATTCACTATTTTTGCCGTCTGAGCTCTTCAATGGTGGAA2220.17647199103331507No Hit
CTCAGTATCCTTCCAGATTCGAAATCAATCCGTGCATCAATTCGGGCTCT2210.17567707215478662No Hit
GTTTACTACTGTCCAGGCCATTGTGCGATCTCTTCCCTCGATTATTTCAA2190.1740872343977297No Hit
GTCCAGGCCATTGTGCGATCTCTTCCCTCGATTATTTCAAATCTGTGCTT2180.17329231551920127No Hit
ATCCAGTATGGTGGAGGCTATGGTTTCCAGAGCCCGAATTGATGCACGGA2150.17090755888361592No Hit
GGTTCCTTGCTCATTGATGAAGTGAAAATCTGAATACATGAAGCATACTT2040.16216345121980302No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1890.15023966804187633No Hit
GTATGGGGAGGACCTGAAAATCGAAACAAACAAATTTGCAGCAATATGCA1840.14626507364923408No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG1810.14388031701364876No Hit
AGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCAC1780.14149556037806343No Hit
ATTGAGGAGTGCCTGATTAATGATCCCTGGGTTTTGCTTAATGCTTCTTG1760.13990572262100653No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGCTAAATTCACTATTTTTGCCG1730.13752096598542118No Hit
GAAGCAGTTTTCTTGATTCAGCTGAAAATCCTTCTAGTTGTGGAGATGCA1720.13672604710689276No Hit
GCTAAATTCACTATTTTTGCCGTCTGAGCTCTTCAATGGTGGAACAGATC1720.13672604710689276No Hit
GAGCGAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT1710.1359311282283643No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA1640.1303666960786652No Hit
GCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTC1620.12877685832160826No Hit
GGTCTTCTGTATGAACTGCTGGGGAAGAATTTTTCAAATAAATTGCAGCA1610.12798193944307984No Hit
CCTTACAGCCATGGGACAGGAACAGGATACACCATGGATACCAGTCGGGA1570.12480226392896605No Hit
GTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGATGTT1570.12480226392896605No Hit
AGCGAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTT1570.12480226392896605No Hit
TCATTAATCAGGCACTCCTCAATTGCTTCATATAGCCCCCCAAGATCAAA1560.12400734505043759No Hit
CTTAGGGACAACCTTGAACCTGGGACCTTTGATCTTGGGGGGCTATATGA1550.12321242617190915No Hit
CTTCCAAGTGAGTGCATATTGCTGCAAATTTGTTTGTTTCGATTTTCAGG1550.12321242617190915No Hit
GTGTAAGGAAGGAGTTGAACCAAGAAGCATTAAGCAAAACCCAGGGATCA1520.12082766953632382No Hit
CACCATGGATACCAGTCGGGATATCCAGTATGGTGGAGGCTATGGTTTCC1480.11764799402221003No Hit
GCAAGGAACCTGGGACCTTTGATCTTGGGGGGCTATATGAAGCAATTGAG1470.11685307514368158No Hit
GGATAAAGAAAGAAGAGTTCACTGAGATCATGAAGATCTGTTCCACCATT1470.11685307514368158No Hit
AGTATGGGGAGGACCTGAAAATCGAAACAAACAAATTTGCAGCAATATGC1460.11605815626515315No Hit
GCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTAAGGAAGGAG1450.1152632373866247No Hit
GAGCAAAAGCAGGGTAGATAATCACTCACTGAGTGACATCAAAATCATGG1440.11446831850809626No Hit
GAGTATGGGGAGGACCTGAAAATCGAAACAAACAAATTTGCAGCAATATG1430.11367339962956781No Hit
ATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTATGA1410.11208356187251091No Hit
GGTATCCATGGTGTATCCTGTTCCTGTCCCATGGCTGTAAGGAGGGTCTC1400.11128864299398246No Hit
GGATACCAGTCGGGATATCCAGTATGGTGGAGGCTATGGTTTCCAGAGCC1400.11128864299398246No Hit
GGATACTGAGATCATGAAGATCTGTTCCACCATTGAAGAGCTCAGACGGC1380.10969880523692557No Hit
GCCACAACTAACTCAATGCATGTGTAAGGAAGGAGTTGAACCAAGAAGCA1370.10890388635839714No Hit
AGTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGT1360.10810896747986869No Hit
AGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTTT1360.10810896747986869No Hit
ATGGATACTGTCAACAGGACACATCAGTACTCAGAAAAGGGAAGATGGAC1330.10572421084428334No Hit
GTATGGTGGAGGCTATGGTTTCCAGAGCCCGAATTGATGCACGGATTGAT1310.10413437308722645No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT1310.10413437308722645No Hit
CTACAGGAGATATGCTTATAAGAAGTGCCATAGGCCAGGTTTCAAGGCCC1300.10333945420869801No Hit
CCTGTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGAT1300.10333945420869801No Hit
GGATAAAGAAAGAAGAGTTCAACAGAAGACCAGTCGGGATATCCAGTCTG1280.1017496164516411No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAG202.1232936E-6145.0145
CGGATTA151.2274696E-4145.0145
CTGCCCT100.007083545145.0145
TACCTCT151.2274696E-4145.0145
AGCAGGC2800.0129.464288
CTGCTTA351.888111E-9124.28571145
GAGCGAA550.0118.636361
GCAGGCA3300.0109.848489
GAGCAAA5200.0108.750011
AGCCCCG203.8581158E-4108.75145
GACGCAC203.8581158E-4108.75145
GCAAAAG5400.0104.722213
CATGGTT1700.089.558835
AAGCAGG7250.087.07
GAAGTAG259.367537E-487.0145
TTACACT259.367537E-487.0145
CAAAAGC6650.085.037594
ACAAGGT755.4569682E-1177.333338
GTAGAAA1700.076.764712
AGTAGAA2000.076.1251
Produced by FastQC (version 0.11.5)