FastQCFastQC Report
Mon 4 Feb 2019
000000000-BTF83_l01_n01_Exp2_SrcID35_Shed_Ind2_D4_rep1_SID49.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BTF83_l01_n01_Exp2_SrcID35_Shed_Ind2_D4_rep1_SID49.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences170984
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGTATCCTTCCAGATTCGAAATCAATCCGTGCATCAATTCGGGCTCTGG28181.6481074252561643No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTT26251.5352313666775839No Hit
GGAACAGGATACACCATGGATACCAGTCGGGATATCCAGTATGGTGGAGG17281.0106208768071867No Hit
CAGTGAACTCTTCTTTCTTTATCCTTCCAGATTCGAAATCAATCCGTGCA16760.9802086744958592No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT13950.8158658120058017No Hit
GGAACATGGTTTCCAGAGCCCGAATTGATGCACGGATTGATTTCGAATCT12650.7398353062274833No Hit
CTCAGACTGGATATCCCGACTGGTCTTCTGTTGAACTCTTCTTTCTTTAT11020.644504748982361No Hit
AGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTCAATCC10730.6275440976933514No Hit
CAGACTGGATATCCCGACTGGTCTTCTGTTGAACTCTTCTTTCTTTATCC9130.5339680905815749No Hit
GTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTA8520.4982922378702101No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAA7280.42577083235858326No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATTCACTATTTT7100.41524353155850835No Hit
TCTCAGTGAACTCTTCTTTCTTTATCCTTCCAGATTCGAAATCAATCCGT6670.39009497964721845No Hit
CTCAGTGAACTCTTCTTTCTTTATCCTTCCAGATTCGAAATCAATCCGTG6200.3626070275581341No Hit
GATGGACAAATGTACCAAAGGTGCTGCAATTTATTTGAAAAATTCTTCCC3970.23218546764609554No Hit
TAGTAGCACTGCCACAACTAACTCAATGCATGTGTAAGGAAGGAGTTGAA3830.2239975670238151No Hit
GAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTT3810.22282786693491788No Hit
CCATGTTCCTGTCCCATGGCTGTAAGGAGGGTCTCCAGTATAAGGGAAAG3760.2199036167126749No Hit
CCATAGCCTCCACCATACTGGATATCCCGACTGGTATCCATGGTGTATCC3540.2070369157348056No Hit
GCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTTTC3420.20001871520142236No Hit
CCATAGCCTCCACCATACTGGATATCCCGACTGGTCTTCTGTATGAACTG3350.1959247648902821No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA3320.19417021475693633No Hit
CACCATGGATACCAGTCGGGATATCCAGTATGGTGGAGGCTATGGTTTCC3210.18773686426800168No Hit
TCTCAGACTGGATATCCCGACTGGTCTTCTGTTGAACTCTTCTTTCTTTA3110.18188836382351564No Hit
CACTTGGAAGTATGCTTCATGTATTCAGATTTTCACTTCATCAATGAGCA3020.1766247134234782No Hit
ATATGAAGCAATTGAGGAGTGCCTGATTAATGATCCCTGGGTTTTGCTTA3020.1766247134234782No Hit
GCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTC2980.1742853132456838No Hit
CTTTTCTTACTGGAGACCCTCCTTACAGCCATGGGACAGGAACAGGATAC2940.1719459130678894No Hit
GTCCAGGCCATTGTGCGATCTCTTCCCTCGATTATTTCAAATCTGTGCTT2790.16317316240116034No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG2780.16258831235671173No Hit
GAAGCAGTTTTCTTGATTCAGCTGAAAATCCTTCTAGTTGTGGAGATGCA2690.1573246619566743No Hit
CCATGGATACCAGTCGGGATATCCAGTATGGTGGAGGCTATGGTTTCCAG2670.1561549618677771No Hit
GAGCGAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT2670.1561549618677771No Hit
ATCCAGTATGGTGGAGGCTATGGTTTCCAGAGCCCGAATTGATGCACGGA2650.1549852617788799No Hit
GAGCAAAAGCAGGGTAGATAATCACTCACTGAGTGACATCAAAATCATGG2620.1532307116455341No Hit
CTCAGTATCCTTCCAGATTCGAAATCAATCCGTGCATCAATTCGGGCTCT2610.15264586160108548No Hit
GGACAAGCTAAATTCACTATTTTTGCCGTCTGAGCTCTTCAATGGTGGAA2550.14913676133439385No Hit
GGTTCCTTGCTCATTGATGAAGTGAAAATCTGAATACATGAAGCATACTT2530.14796706124549666No Hit
GTATGGGGAGGACCTGAAAATCGAAACAAACAAATTTGCAGCAATATGCA2500.14621251111215083No Hit
GTTTACTACTGTCCAGGCCATTGTGCGATCTCTTCCCTCGATTATTTCAA2480.14504281102325364No Hit
AGCGAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTT2440.14270341084545923No Hit
GCTAAATTCACTATTTTTGCCGTCTGAGCTCTTCAATGGTGGAACAGATC2360.1380246104898704No Hit
AGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTTT2220.12983670986758994No Hit
AGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCAC2170.12691245964534692No Hit
ATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTATGA2120.1239882094231039No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGCTAAATTCACTATTTTTGCCG2120.1239882094231039No Hit
ATTGAGGAGTGCCTGATTAATGATCCCTGGGTTTTGCTTAATGCTTCTTG2120.1239882094231039No Hit
GTAGTAAACAGTATTTGCAACACTACAGGAGATATGCTTATAAGAAGTGC2100.12281850933420671No Hit
CCTTACAGCCATGGGACAGGAACAGGATACACCATGGATACCAGTCGGGA2080.1216488092453095No Hit
GTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGATGTT2040.1193094090675151No Hit
GAACAGGATACACCATGGATACCAGTCGGGATATCCAGTATGGTGGAGGC2000.11697000888972067No Hit
AACTAACTCAATGCATGTGTAAGGAAGGAGTTGAACCAAGAAGCATTAAG1960.11463060871192626No Hit
GCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTAAGGAAGGAG1950.11404575866747765No Hit
GGATACACCATGGATACTGTCAACAGGACACATCAGTACTCAGAAAAGGG1950.11404575866747765No Hit
AGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTTT1930.11287605857858045No Hit
CCTGTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGAT1930.11287605857858045No Hit
GGATAAAGAAAGAAGAGTTCACTGAGATCATGAAGATCTGTTCCACCATT1930.11287605857858045No Hit
GTGTAAGGAAGGAGTTGAACCAAGAAGCATTAAGCAAAACCCAGGGATCA1880.10995180835633744No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1880.10995180835633744No Hit
GCAAGGAACCTGGGACCTTTGATCTTGGGGGGCTATATGAAGCAATTGAG1850.10819725822299163No Hit
ATGGATACTGTCAACAGGACACATCAGTACTCAGAAAAGGGAAGATGGAC1840.107612408178543No Hit
CTTCCAAGTGAGTGCATATTGCTGCAAATTTGTTTGTTTCGATTTTCAGG1840.107612408178543No Hit
AGCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTT1810.10585785804519722No Hit
GGAACAGGATACACCATGGATACTGTCAACAGGACACATCAGTACTCAGA1790.1046881579563No Hit
TCATTAATCAGGCACTCCTCAATTGCTTCATATAGCCCCCCAAGATCAAA1770.1035184578674028No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT1770.1035184578674028No Hit
GGATACTGTCAACAGGACACATCAGTACTCAGAAAAGGGAAGATGGACAA1760.1029336078229542No Hit
GGTCTTCTGTATGAACTGCTGGGGAAGAATTTTTCAAATAAATTGCAGCA1750.10234875777850559No Hit
GGATACTGAGATCATGAAGATCTGTTCCACCATTGAAGAGCTCAGACGGC1720.10059420764515978No Hit
AGTATGGGGAGGACCTGAAAATCGAAACAAACAAATTTGCAGCAATATGC1710.10000935760071118No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCTCCG100.007085757145.0145
GAGCGAA950.0129.736851
TACTTCA301.0977601E-7120.833336145
AGCAGGC4200.0113.9285668
GAGCAAA6850.0112.189781
TGAAGTA203.860516E-4108.75145
CGGATTA203.860516E-4108.75145
TCTCCGC352.7518763E-7103.57143145
GCAAAAG7450.0102.1812063
TCGTATC750.096.66667145
TAAACTA301.59565E-596.6666645
GCAGGAG301.59565E-596.6666649
GCAGGCA5150.092.912629
CACGGGC406.09567E-790.625145
CAATAGT406.09567E-790.6259
CAAAAGC8450.090.088764
AAGCAGG10150.087.142867
TTTCCTA259.373345E-487.0145
CGGGCTT259.373345E-487.0145
CTCTACT259.373345E-487.0145
Produced by FastQC (version 0.11.5)