FastQCFastQC Report
Mon 4 Feb 2019
000000000-BTF83_l01_n01_Exp2_SrcID34_Shed_Ind1_D4_rep2_SID65.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BTF83_l01_n01_Exp2_SrcID34_Shed_Ind1_D4_rep2_SID65.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences121705
Sequences flagged as poor quality0
Sequence length151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT24802.037714144858469No Hit
AGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTCAATCC17051.4009284745901975No Hit
GTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTA13551.1133478493077524No Hit
GAGCAAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAA13131.0788381742738589No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAA12841.0550100653218848No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTT12831.0541884063925064No Hit
TTTTTAAACTATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG6960.5718746148473768No Hit
ATTATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCA6870.5644796844829711No Hit
TAGTAGCACTGCCACAACTAACTCAATGCATGTGTAAGGAAGGAGTTGAA5460.44862577544061455No Hit
GAAGCAGTTTTCTTGATTCAGCTGAAAATCCTTCTAGTTGTGGAGATGCA5340.4387658682880736No Hit
ATATGAAGCAATTGAGGAGTGCCTGATTAATGATCCCTGGGTTTTGCTTA4980.4091861468304507No Hit
GCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTC4850.39850458074853123No Hit
GAGCGAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT4720.3878230146666119No Hit
GTATGGGGAGGACCTGAAAATCGAAACAAACAAATTTGCAGCAATATGCA4640.38124974323158456No Hit
CTTAGGGACAACCTTGAACCTGGGACCTTTGATCTTGGGGGGCTATATGA4060.33359352532763653No Hit
CAATTAGCCTTCTCTCCTTTCGCAAGGTTGCTCAGTTCATTGATGCTTAG3900.32044698245758185No Hit
ATTGAGGAGTGCCTGATTAATGATCCCTGGGTTTTGCTTAATGCTTCTTG3750.3081220985169056No Hit
GTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACCACGTCTCCTTGC3650.2999055092231215No Hit
AGCGAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTT3570.2933322377880942No Hit
AGCGAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAAG3550.2916889199293373No Hit
GAATAAAAGAACTAAGAAATCTAATGTCGCAGTCTCGCACCCGCGAGATA3540.2908672609999589No Hit
GCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTAAGGAAGGAG3540.2908672609999589No Hit
GCTTCATATAGCCCCCCAAGATCAAAGGTCCCAGGTTCAAGGTTGTCCCT3480.2859373074236884No Hit
GAGCGAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAA3430.28182901277679634No Hit
ATATGGCCATAATCAAGAAGTACACATCAGGAAGACAGGAGAAGAACCCA3330.2736124234830122No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT3250.2670391520479849No Hit
AGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCAC3250.2670391520479849No Hit
CAATTAGCACATTAGCCTTCTCTCCTTTCGCAAGGTTGCTCAGTTCATTG3230.2653958341892281No Hit
ACCTTGAACCTGGGACCTTTGATCTTGGGGGGCTATATGAAGCAATTGAG3140.2580009038248223No Hit
TCATTAATCAGGCACTCCTCAATTGCTTCATATAGCCCCCCAAGATCAAA3140.2580009038248223No Hit
AAACAAGGTCGTTTTTAAACTATTCGACACTAATTGATGGCCATCCGAAT3100.25471426810730863No Hit
GTGTAAGGAAGGAGTTGAACCAAGAAGCATTAAGCAAAACCCAGGGATCA3060.251427632389795No Hit
GCAGAAGGATTTTCAGCTGAATCAAGAAAACTGCTTCTTATCGTTCAGGC3060.251427632389795No Hit
AGCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTT3030.24896265560165973No Hit
GCTTGTATGCATCTCCACAACTAGAAGGATTTTCAGCTGAATCAAGAAAA3000.24649767881352452No Hit
AACTAACTCAATGCATGTGTAAGGAAGGAGTTGAACCAAGAAGCATTAAG2900.23828108951974036No Hit
GCCACAACTAACTCAATGCATGTGTAAGGAAGGAGTTGAACCAAGAAGCA2890.23745943059036195No Hit
GCAAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAAGA2880.23663777166098351No Hit
GTAGAAACAAGGTCGTTTTTAAACTATTCGACACTAATTGATGGCCATCC2800.2300645002259562No Hit
GTTCATGCTCCAGTTTCGGTGTTTGTTGTCCATCTTCCCTTTTCTGAGTA2800.2300645002259562No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATTCACTATTTT2730.22431288772030727No Hit
AGTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGT2730.22431288772030727No Hit
GTTCAAGGTTGTCCCTAAGAGCCTGAACGATAAGAAGCAGTTTTCTTGAT2720.22349122879092886No Hit
CCCTTATACTGGAGACCCTCCTTACAGCCATGGGACAGGAACAGGATACA2660.2185612752146584No Hit
GTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCACT2630.21609629842652317No Hit
AGTATGGGGAGGACCTGAAAATCGAAACAAACAAATTTGCAGCAATATGC2630.21609629842652317No Hit
CTCCTCAATTGCTTCATATAGCCCCCCAAGATCAAAGGTCCCAGGTTCAA2580.21198800377963106No Hit
GTCCCTAAGAGCCTGAACGATAAGAAGCAGTTTTCTTGATTCAGCTGAAA2570.21116634485025265No Hit
GGCTAATTGGGCAAGGAGACGTGGTGTTGGTAATGAAACGGAAACGGGAC2500.20541473234460375No Hit
ATACTGGATATCCCGACTGGTCTTCTGTATGAACTGCTGGGGAAGAATTT2480.20377141448584693No Hit
CTTCCAAGTGAGTGCATATTGCTGCAAATTTGTTTGTTTCGATTTTCAGG2470.20294975555646852No Hit
CCTCAATTGCTTCATATAGCCCCCCAAGATCAAAGGTCCCAGGTTCAAGG2440.2004847787683333No Hit
GGTTCAAGGTTGTCCCTAAGAGCCTGAACGATAAGAAGCAGTTTTCTTGA2390.19637648412144118No Hit
GGAACTTTCCTCCACTCCTTTGGGGGACTCTCCAATGGGCCATGTTTCTG2380.19555482519206277No Hit
GGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATG2360.19391150733330595No Hit
AGCAAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAAG2270.1865165769689002No Hit
GGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTAAGGA2250.18487325911014338No Hit
CCATGTCAACAGGACACATCAGTACTCAGAAAAGGGAAGATGGACAACAA2230.18322994125138653No Hit
GAGTATGGGGAGGACCTGAAAATCGAAACAAACAAATTTGCAGCAATATG2200.1807649644632513No Hit
GCAATATGCACTCACTTGGAAGTATGCTTCATGTAACTAGAAGGATTTTC2190.1799433055338729No Hit
ATACTGGAGACCCTCCTTACAGCCATGGGACAGGAACAGGATACACCATG2190.1799433055338729No Hit
TCAATGAACTGAGCAACCTTGCGAAAGGAGAGAAGGCTAATGTGCTAATT2180.17912164660449448No Hit
ATATAGCCCCCCAAGATCAAAGGTCCCAGGTTCAAGGTTGTCCCTAAGAG2150.17665666981635922No Hit
CACTTGGAAGTATGCTTCATGTATTCAGATTTTCACTTCATCAATGAGCA2100.17254837516946714No Hit
GTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTCAATCCGATGA2090.17172671624008873No Hit
GCTTGTGGGGGGCTATATGAAGCAATTGAGGAGTGCCTGATTAATGATCC2090.17172671624008873No Hit
GAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTT2090.17172671624008873No Hit
GGCTAATGTGCTAATTGGGCAAGGAGACGTGGTGTTGGTAATGAAACGGA2050.1684400805225751No Hit
TCCCCATACTCTTTCATTGTTTTTTCCGCAAGCTCGACAATCATCGGATT1990.1635101269463046No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGTGAACTCTTCTTTCTTT1970.16186680908754777No Hit
ATCAAGAAGTACACATCAGGAAGACAGGAGAAGAACCCAGCACTTAGGAT1960.16104515015816934No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT1950.16022349122879093No Hit
CAGTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTG1910.15693685551127726No Hit
GGCCAGCACTAAGCATCAATGAACTGAGCAACCTTGCGAAAGGAGAGAAG1890.15529353765252044No Hit
GGATACACCATGGATACTGTCAACAGGACACATCAGTACTCAGAAAAGGG1890.15529353765252044No Hit
GTTCATTGATTCAATTTCTTTCTGTTTGGGCATAACCACTTGGTTCATTG1850.15200690193500677No Hit
GCATTAAGCAAAACCCAGGGATCATTAATCAGGCACTCCTCAATTGCTTC1850.15200690193500677No Hit
ATCAGGCACTCCTCAATTGCTTCATATAGCCCCCCAAGATCAAAGGTCCC1840.15118524300562838No Hit
ATACCGACTTTGCTAATATTTCAAATCTGTGCTTCAAAAGTGCATTTGGA1840.15118524300562838No Hit
TTCTTTATCCTTCCAGATTCGAAATCAATCCGTGCATCAATTCGGGCTCT1840.15118524300562838No Hit
CAACTAACTCAATGCATGTGTAAGGAAGGAGTTGAACCAAGAAGCATTAA1820.14954192514687153No Hit
ATGCATGTGTAAGGAAGGAGTTGAACCAAGAAGCATTAAGCAAAACCCAG1820.14954192514687153No Hit
TGTGTAAGGAAGGAGTTGAACCAAGAAGCATTAAGCAAAACCCAGGGATC1770.14543363049997945No Hit
ATCTAATGTCGCAGTCTCGCACCCGCGAGATACTCACAAAAACCACCGTG1760.14461197157060104No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACCACGT1730.1421469947824658No Hit
CTCAATGCATGTGTAAGGAAGGAGTTGAACCAAGAAGCATTAAGCAAAAC1710.14050367692370896No Hit
GAAAAATGAAAGAGTATGGGGAGGACCTGAAAATCGAAACAAACAAATTT1710.14050367692370896No Hit
CCTTACAGCCATGGGACAGGAACAGGATACACCATGGATACTGTCAACAG1700.13968201799433055No Hit
ATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCAGTC1690.13886035906495214No Hit
CCTTGAACCTGGGACCTTTGATCTTGGGGGGCTATATGAAGCAATTGAGG1690.13886035906495214No Hit
GCAATATGCACTCACTTGGAAGTATGCTTCATGTATTCAGATTTTCACTT1620.13310874655930324No Hit
CATGTAACTAGAAGGATTTTCAGCTGAATCAAGAAAACTGCTTCTTATCG1620.13310874655930324No Hit
CTCACTTGGAAGTATGCTTCTGAGAACAAATCAGAAACATGGCCCATTGG1610.1322870876299248No Hit
GCATACTTCCAAGTGAGTGCATATTGCTGCAAATTTGTTTGTTTCGATTT1600.1314654287005464No Hit
ATTGGAGAGTCCCCCAAAGGAGTGGAGGAAAGTTCCATTGGGAAGGTCTG1600.1314654287005464No Hit
CCATGTTTTTGGCTGGACCATGTGCTGGCATCATCACTGCATTGTTCATG1570.12900045191241116No Hit
AAATGAAAGAGTATGGGGAGGACCTGAAAATCGAAACAAACAAATTTGCA1570.12900045191241116No Hit
ACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG1550.12735713405365431No Hit
GGAAAGTTCCATTGGGAAGGTCTGCAGGACTTTATTAGCAAAGTCGGTAT1550.12735713405365431No Hit
GCTTCAATCCGATGATTGTCGAGCTTGCGGAAAAAACAATGAAAGAGTAT1550.12735713405365431No Hit
GCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTTTC1540.12653547512427593No Hit
GATTAATGATCCCTGGGTTTTGCTTAATGCTTCTTGGTTCAACTCCTTCC1530.1257138161948975No Hit
ATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACCACGTCTCCTTGCCC1510.12407049833614067No Hit
AAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCACTGCCA1500.12324883940676226No Hit
CTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAA1490.12242718047738384No Hit
GATCAAAGGTCCCAGGTTCAAGGTTGTCCCTAAGAGCCTGAACGATAAGA1490.12242718047738384No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG1480.12160552154800543No Hit
GTTGACAGTATCCATGGTGTATCCTGTTCCTGTCCCATGGCTGTAAGGAG1480.12160552154800543No Hit
CCTTCTGCTGCAAATTTGTTTGTTTCGATTTTCAGGTCCTCCCCATACTC1470.120783862618627No Hit
GGGCCATGTTTCTGATTTGTTCTCAAAGAACTCTTTGGTCATGTCTTTCT1470.120783862618627No Hit
ACCCAGGGATCATTAATCAGGCACTCCTCAATTGCTTCATATAGCCCCCC1450.11914054475987018No Hit
CCATATGGCCATAATCAAGAAGTACACATCAGGAAGACAGGAGAAGAACC1390.11421059118359969No Hit
TCCTTCTGCTGCAAATTTGTTTGTTTCGATTTTCAGGTCCTCCCCATACT1390.11421059118359969No Hit
GATCCAAAATGGAAGATTTTGTGCGACAATGCTTCAATCCGATGATTGTC1390.11421059118359969No Hit
GTACACATCAGGAAGACAGGAGAAGAACCCAGCACTTAGGATGAAATGGA1380.11338893225422127No Hit
AACTCAATGCATGTGTAAGGAAGGAGTTGAACCAAGAAGCATTAAGCAAA1370.11256727332484287No Hit
GTATGCATCTCCACAACTAGAAGGATTTTCAGCTGAATCAAGAAAACTGC1370.11256727332484287No Hit
CCCCATACTCTTTCATTGTTTTTTCCGCAAGCTCGACAATCATCGGATTG1360.11174561439546443No Hit
CTCGATTGAAGCTGAGTCCTCTGTCAAAGAGAAAGACATGACCAAAGAGT1350.11092395546608604No Hit
AGTAGAAACAAGGTCGTTTTTAAACTATTCGACACTAATTGATGGCCATC1340.11010229653670761No Hit
TCCCTGGGTTTTGCTTAATGCTTCTTGGTTCAACTCCTTCCTTACACATG1330.1092806376073292No Hit
AGTTGTGGAGATGCATACAAGCTGTTGAATACCGACTTTGCTAATAAAGT1330.1092806376073292No Hit
CCATGGATACTGTCAACAGGACACATCAGTACTCAGAAAAGGGAAGATGG1310.10763731974857238No Hit
CCCACAAGCTGTTGAATACCGACTTTGCTAATAAAGTCCTGCAGACCTTC1310.10763731974857238No Hit
GGGCCATGTTTCTGATTTGTTCTCAGAAGCATACTTCCAAGTGAGTGCAT1290.10599400188981553No Hit
ATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCAGTCTC1280.10517234296043712No Hit
GTTCATTGATGCTTAGTGCTGGCCCATATCTCTTGTCTTCTTTGTCTGCT1280.10517234296043712No Hit
GTGCTGGCCCATATCTCTTGTCTTCTTTGTCTGCTGTAATTGGATATTTC1260.1035290251016803No Hit
GCTTCTTATCGTTCAGGCTCTTAGGGACAACCTTGAACCTGGGACCTTTG1250.10270736617230188No Hit
GCACTAAGCATCAATGAACTGAGCAACCTTGCGAAAGGAGAGAAGGCTAA1240.10188570724292347No Hit
CAAAGAACTCTTTGGTCATGTCTTTCTCTTTGACAGAGGACTCAGCTTCA1240.10188570724292347No Hit
GGACAAGCTAAATTCACTATTTTTGCCGTCTGAGCTCTTCAATGGTGGAA1240.10188570724292347No Hit
TCATGTAACTAGAAGGATTTTCAGCTGAATCAAGAAAACTGCTTCTTATC1230.10106404831354504No Hit
ACAATGCTTCAATCCGATGATTGTCGAGCTTGCGGAAAAAACAATGAAAG1220.10024238938416664No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCTTA151.2273731E-4145.0145
GGTTTAT100.0070832656145.0145
GGATACG100.0070832656145.0145
AGTATAT100.0070832656145.02
GAGCGAA1150.0145.01
ACTAATA100.0070832656145.08
GATGAGC100.0070832656145.0145
AATAGGT100.0070832656145.07
TAGTATA100.0070832656145.01
GAGCAAA6200.0134.47581
TAGTTGG256.4419037E-6116.0145
GCTACGT203.857813E-4108.75145
GCAAAAG7850.0106.210193
CAAAAGC8150.0102.300624
TTCAGCG501.9281288E-10101.5145
AGCAGGC2100.0100.119058
GCAGGTC3050.095.081979
AAAAGCA8950.093.1564255
AAGCAGG10850.092.211987
GGAAACT406.085029E-790.625145
Produced by FastQC (version 0.11.5)