FastQCFastQC Report
Mon 4 Feb 2019
000000000-BTF83_l01_n01_Exp2_SrcID34_Shed_Ind1_D4_rep1_SID48.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BTF83_l01_n01_Exp2_SrcID34_Shed_Ind1_D4_rep1_SID48.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences145537
Sequences flagged as poor quality0
Sequence length151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT27111.862756549880786No Hit
AGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTCAATCC21731.493091103980431No Hit
GAGCAAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAA15771.0835732494142383No Hit
GTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTA15141.0402852882772078No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAA14901.023794636415482No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTT12930.888433869050482No Hit
TTTTTAAACTATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG9410.6465709750785024No Hit
ATTATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCA8820.6060314559184262No Hit
GCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTC6920.47548046201309635No Hit
TAGTAGCACTGCCACAACTAACTCAATGCATGTGTAAGGAAGGAGTTGAA6660.4576155891628933No Hit
GAAGCAGTTTTCTTGATTCAGCTGAAAATCCTTCTAGTTGTGGAGATGCA5710.3923400922102283No Hit
ATATGAAGCAATTGAGGAGTGCCTGATTAATGATCCCTGGGTTTTGCTTA5380.3696654459003552No Hit
GAGCGAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT5250.3607330094752537No Hit
AGCGAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAAG5160.3545490150271065No Hit
GTATGGGGAGGACCTGAAAATCGAAACAAACAAATTTGCAGCAATATGCA5080.3490521310731979No Hit
CAATTAGCCTTCTCTCCTTTCGCAAGGTTGCTCAGTTCATTGATGCTTAG5020.3449294681077664No Hit
CTTAGGGACAACCTTGAACCTGGGACCTTTGATCTTGGGGGGCTATATGA4700.32294193229213186No Hit
GTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACCACGTCTCCTTGC4520.3105739433958375No Hit
AGCGAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTT4190.2878992970859644No Hit
GAATAAAAGAACTAAGAAATCTAATGTCGCAGTCTCGCACCCGCGAGATA4170.28652507609748723No Hit
ATTGAGGAGTGCCTGATTAATGATCCCTGGGTTTTGCTTAATGCTTCTTG4170.28652507609748723No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT4140.2844637446147715No Hit
GCAAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAAGA4020.27621841868390856No Hit
AACTAACTCAATGCATGTGTAAGGAAGGAGTTGAACCAAGAAGCATTAAG3960.2720957557184771No Hit
GTGTAAGGAAGGAGTTGAACCAAGAAGCATTAAGCAAAACCCAGGGATCA3880.2665988717645684No Hit
CAATTAGCACATTAGCCTTCTCTCCTTTCGCAAGGTTGCTCAGTTCATTG3870.2659117612703299No Hit
ATATGGCCATAATCAAGAAGTACACATCAGGAAGACAGGAGAAGAACCCA3870.2659117612703299No Hit
AGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCAC3850.2645375402818527No Hit
GCAGAAGGATTTTCAGCTGAATCAAGAAAACTGCTTCTTATCGTTCAGGC3780.2597277668221827No Hit
CTCCTCAATTGCTTCATATAGCCCCCCAAGATCAAAGGTCCCAGGTTCAA3610.24804688842012687No Hit
GAGCGAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAA3580.24598555693741112No Hit
GTAGAAACAAGGTCGTTTTTAAACTATTCGACACTAATTGATGGCCATCC3580.24598555693741112No Hit
ACCTTGAACCTGGGACCTTTGATCTTGGGGGGCTATATGAAGCAATTGAG3570.24529844644317253No Hit
GCTTCATATAGCCCCCCAAGATCAAAGGTCCCAGGTTCAAGGTTGTCCCT3570.24529844644317253No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATTCACTATTTT3550.24392422545469536No Hit
GTTCAAGGTTGTCCCTAAGAGCCTGAACGATAAGAAGCAGTTTTCTTGAT3480.2391144519950253No Hit
GCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTAAGGAAGGAG3440.236366010018071No Hit
TCATTAATCAGGCACTCCTCAATTGCTTCATATAGCCCCCCAAGATCAAA3390.2329304575468781No Hit
AGCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTT3320.22812068408720806No Hit
AAACAAGGTCGTTTTTAAACTATTCGACACTAATTGATGGCCATCCGAAT3280.22537224211025375No Hit
AGCAAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAAG3250.223310910627538No Hit
AGTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGT3230.22193668963906085No Hit
GCCACAACTAACTCAATGCATGTGTAAGGAAGGAGTTGAACCAAGAAGCA3230.22193668963906085No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3210.2205624686505837No Hit
GTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCACT3200.21987535815634512No Hit
GCTTGTATGCATCTCCACAACTAGAAGGATTTTCAGCTGAATCAAGAAAA3160.2171269161793908No Hit
ATACTGGATATCCCGACTGGTCTTCTGTATGAACTGCTGGGGAAGAATTT3150.21643980568515223No Hit
GTTCATGCTCCAGTTTCGGTGTTTGTTGTCCATCTTCCCTTTTCTGAGTA3070.21094292173124363No Hit
AGTATGGGGAGGACCTGAAAATCGAAACAAACAAATTTGCAGCAATATGC2990.20544603777733497No Hit
ATATAGCCCCCCAAGATCAAAGGTCCCAGGTTCAAGGTTGTCCCTAAGAG2950.2026975958003807No Hit
GGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATG2950.2026975958003807No Hit
GGCTAATGTGCTAATTGGGCAAGGAGACGTGGTGTTGGTAATGAAACGGA2940.2020104853061421No Hit
GGAACTTTCCTCCACTCCTTTGGGGGACTCTCCAATGGGCCATGTTTCTG2880.19788782234071062No Hit
CCCTTATACTGGAGACCCTCCTTACAGCCATGGGACAGGAACAGGATACA2880.19788782234071062No Hit
GGCTAATTGGGCAAGGAGACGTGGTGTTGGTAATGAAACGGAAACGGGAC2850.19582649085799486No Hit
GTCCCTAAGAGCCTGAACGATAAGAAGCAGTTTTCTTGATTCAGCTGAAA2850.19582649085799486No Hit
CTTCCAAGTGAGTGCATATTGCTGCAAATTTGTTTGTTTCGATTTTCAGG2800.19239093838680196No Hit
GGCCAGCACTAAGCATCAATGAACTGAGCAACCTTGCGAAAGGAGAGAAG2750.1889553859156091No Hit
CAACTAACTCAATGCATGTGTAAGGAAGGAGTTGAACCAAGAAGCATTAA2720.18689405443289336No Hit
ATACCGACTTTGCTAATATTTCAAATCTGTGCTTCAAAAGTGCATTTGGA2660.18277139146746188No Hit
CCTCAATTGCTTCATATAGCCCCCCAAGATCAAAGGTCCCAGGTTCAAGG2580.17727450751355325No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT2520.1731518445481218No Hit
GAGTATGGGGAGGACCTGAAAATCGAAACAAACAAATTTGCAGCAATATG2510.1724647340538832No Hit
TCAATGAACTGAGCAACCTTGCGAAAGGAGAGAAGGCTAATGTGCTAATT2500.17177762355964463No Hit
GCAATATGCACTCACTTGGAAGTATGCTTCATGTAACTAGAAGGATTTTC2480.17040340257116746No Hit
ATCAAGAAGTACACATCAGGAAGACAGGAGAAGAACCCAGCACTTAGGAT2440.16765496059421317No Hit
CCATGTCAACAGGACACATCAGTACTCAGAAAAGGGAAGATGGACAACAA2420.166280739605736No Hit
ATCAGGCACTCCTCAATTGCTTCATATAGCCCCCCAAGATCAAAGGTCCC2410.16559362911149741No Hit
GGTTCAAGGTTGTCCCTAAGAGCCTGAACGATAAGAAGCAGTTTTCTTGA2410.16559362911149741No Hit
GGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTAAGGA2400.16490651861725883No Hit
ATACTGGAGACCCTCCTTACAGCCATGGGACAGGAACAGGATACACCATG2340.16078385565182737No Hit
ATCTAATGTCGCAGTCTCGCACCCGCGAGATACTCACAAAAACCACCGTG2310.15872252416911165No Hit
CAGTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTG2310.15872252416911165No Hit
CACTTGGAAGTATGCTTCATGTATTCAGATTTTCACTTCATCAATGAGCA2290.15734830318063447No Hit
CTCAATGCATGTGTAAGGAAGGAGTTGAACCAAGAAGCATTAAGCAAAAC2290.15734830318063447No Hit
GAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTT2240.15391275070944158No Hit
ATGCATGTGTAAGGAAGGAGTTGAACCAAGAAGCATTAAGCAAAACCCAG2230.15322564021520302No Hit
GTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTCAATCCGATGA2220.15253852972096443No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACCACGT2200.15116430873248726No Hit
GCATTAAGCAAAACCCAGGGATCATTAATCAGGCACTCCTCAATTGCTTC2200.15116430873248726No Hit
GGAAAGTTCCATTGGGAAGGTCTGCAGGACTTTATTAGCAAAGTCGGTAT2190.1504771982382487No Hit
TTCTTTATCCTTCCAGATTCGAAATCAATCCGTGCATCAATTCGGGCTCT2170.14910297724977153No Hit
TGTGTAAGGAAGGAGTTGAACCAAGAAGCATTAAGCAAAACCCAGGGATC2150.14772875626129436No Hit
ATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCAGTC2140.14704164576705578No Hit
ACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG2110.14498031428434008No Hit
TCCCCATACTCTTTCATTGTTTTTTCCGCAAGCTCGACAATCATCGGATT2080.14291898280162432No Hit
AAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCACTGCCA2080.14291898280162432No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGTGAACTCTTCTTTCTTT2040.14017054082467No Hit
ACCCAGGGATCATTAATCAGGCACTCCTCAATTGCTTCATATAGCCCCCC1990.13673498835347714No Hit
CCCACAAGCTGTTGAATACCGACTTTGCTAATAAAGTCCTGCAGACCTTC1960.13467365687076138No Hit
GCTTCAATCCGATGATTGTCGAGCTTGCGGAAAAAACAATGAAAGAGTAT1960.13467365687076138No Hit
CCTTGAACCTGGGACCTTTGATCTTGGGGGGCTATATGAAGCAATTGAGG1950.1339865463765228No Hit
CCTTCTGCTGCAAATTTGTTTGTTTCGATTTTCAGGTCCTCCCCATACTC1880.12917677291685276No Hit
GTTGACAGTATCCATGGTGTATCCTGTTCCTGTCCCATGGCTGTAAGGAG1840.12642833093989844No Hit
AGTAGAAACAAGGTCGTTTTTAAACTATTCGACACTAATTGATGGCCATC1810.1243669994571827No Hit
GATTAATGATCCCTGGGTTTTGCTTAATGCTTCTTGGTTCAACTCCTTCC1790.12299277846870556No Hit
GAAAAATGAAAGAGTATGGGGAGGACCTGAAAATCGAAACAAACAAATTT1790.12299277846870556No Hit
GCACTAAGCATCAATGAACTGAGCAACCTTGCGAAAGGAGAGAAGGCTAA1780.12230566797446697No Hit
CCATATGGCCATAATCAAGAAGTACACATCAGGAAGACAGGAGAAGAACC1780.12230566797446697No Hit
GTTCATTGATGCTTAGTGCTGGCCCATATCTCTTGTCTTCTTTGTCTGCT1770.1216185574802284No Hit
CTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAA1770.1216185574802284No Hit
CTCACTTGGAAGTATGCTTCTGAGAACAAATCAGAAACATGGCCCATTGG1760.12093144698598982No Hit
GTACACATCAGGAAGACAGGAGAAGAACCCAGCACTTAGGATGAAATGGA1760.12093144698598982No Hit
CCTTACAGCCATGGGACAGGAACAGGATACACCATGGATACTGTCAACAG1740.11955722599751265No Hit
GCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTTTC1720.1181830050090355No Hit
GTGCTGGCCCATATCTCTTGTCTTCTTTGTCTGCTGTAATTGGATATTTC1690.11612167352631976No Hit
GGATACACCATGGATACTGTCAACAGGACACATCAGTACTCAGAAAAGGG1690.11612167352631976No Hit
GCAATATGCACTCACTTGGAAGTATGCTTCATGTATTCAGATTTTCACTT1670.11474745253784262No Hit
GGGCCATGTTTCTGATTTGTTCTCAAAGAACTCTTTGGTCATGTCTTTCT1640.11268612105512688No Hit
AAATGAAAGAGTATGGGGAGGACCTGAAAATCGAAACAAACAAATTTGCA1630.11199901056088829No Hit
ATTAGCACATTAGCCTTCTCTCCTTTCGCAAGGTTGCTCAGTTCATTGAT1600.10993767907817256No Hit
ATCCAGTATGGTGGAGGCTATGGTTTCCAGAGCCCGAATTGATGCACGGA1590.10925056858393399No Hit
CCATGTTTTTGGCTGGACCATGTGCTGGCATCATCACTGCATTGTTCATG1580.1085634580896954No Hit
AATTAGCACATTAGCCTTCTCTCCTTTCGCAAGGTTGCTCAGTTCATTGA1570.10787634759545682No Hit
GATCAAAGGTCCCAGGTTCAAGGTTGTCCCTAAGAGCCTGAACGATAAGA1570.10787634759545682No Hit
GCTTGTGGGGGGCTATATGAAGCAATTGAGGAGTGCCTGATTAATGATCC1550.10650212660697966No Hit
CCCCATACTCTTTCATTGTTTTTTCCGCAAGCTCGACAATCATCGGATTG1530.10512790561850252No Hit
AACTCAATGCATGTGTAAGGAAGGAGTTGAACCAAGAAGCATTAAGCAAA1530.10512790561850252No Hit
ATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACCACGTCTCCTTGCCC1520.10444079512426394No Hit
GTTCATTGATTCAATTTCTTTCTGTTTGGGCATAACCACTTGGTTCATTG1520.10444079512426394No Hit
GTAAGGAAGGAGTTGAACCAAGAAGCATTAAGCAAAACCCAGGGATCATT1520.10444079512426394No Hit
GCATACTTCCAAGTGAGTGCATATTGCTGCAAATTTGTTTGTTTCGATTT1520.10444079512426394No Hit
GATCCAAAATGGAAGATTTTGTGCGACAATGCTTCAATCCGATGATTGTC1520.10444079512426394No Hit
CAATTGAGGAGTGCCTGATTAATGATCCCTGGGTTTTGCTTAATGCTTCT1510.10375368463002535No Hit
CATCCATTTCATCCTAAGTGCTGGGTTCTTCTCCTGTCTTCCTGATGTGT1500.10306657413578678No Hit
TCATGTAACTAGAAGGATTTTCAGCTGAATCAAGAAAACTGCTTCTTATC1500.10306657413578678No Hit
AGTTACATGAAGCATACTTCCAAGTGAGTGCATATTGCTGCAAATTTGTT1490.1023794636415482No Hit
GTATGCATCTCCACAACTAGAAGGATTTTCAGCTGAATCAAGAAAACTGC1490.1023794636415482No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG1480.10169235314730961No Hit
GCTTCTTATCGTTCAGGCTCTTAGGGACAACCTTGAACCTGGGACCTTTG1470.10100524265307105No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGGTT151.227863E-4145.0145
TACGGAG100.0070846803145.0145
CCCGTTA100.0070846803145.0145
GAGCGAA1300.0133.846161
GAGCAAA7100.0120.492951
CTCTTAA650.0111.53847145
AGCCCCG203.8593478E-4108.75145
TTAAGCG203.8593478E-4108.75145
TTAGTAG203.8593478E-4108.751
ATACACT600.0108.75145
AGCAGGC2200.0102.159098
AGCCAGT301.5948472E-596.6666646
TCGATTA301.5948472E-596.666664145
GTAGCCA301.5948472E-596.6666644
CCGTAGC301.5948472E-596.6666642
GCCGTAG301.5948472E-596.6666641
GCAAAAG8950.096.3966453
AGTAGAA1700.093.8235241
CAAAAGC9150.093.497274
AAGCAGG11600.087.57
Produced by FastQC (version 0.11.5)