FastQCFastQC Report
Mon 4 Feb 2019
000000000-BTF83_l01_n01_Exp2_SrcID32_Shed_Ind1_D3_rep2_SID63.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BTF83_l01_n01_Exp2_SrcID32_Shed_Ind1_D3_rep2_SID63.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42733
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTAAACTATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG5071.1864367116748182No Hit
ATTATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCA4030.9430650785107528No Hit
GAGCAAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAA3940.9220040717946317No Hit
GAATAAAAGAACTAAGAAATCTAATGTCGCAGTCTCGCACCCGCGAGATA2990.6996934453466875No Hit
ATCTAATGTCGCAGTCTCGCACCCGCGAGATACTCACAAAAACCACCGTG2700.6318302014836309No Hit
AGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTCAATCC2490.5826878524793485No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT2310.5405658390471064No Hit
TCAATGAACTGAGCAACCTTGCGAAAGGAGAGAAGGCTAATGTGCTAATT2240.524185056045679No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT2180.5101443849015983No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT2180.5101443849015983No Hit
GTAGAAACAAGGTCGTTTTTAAACTATTCGACACTAATTGATGGCCATCC2150.503124049329558No Hit
GTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACCACGTCTCCTTGC1970.46100203589731586No Hit
AGCGAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAAG1880.43994102918119493No Hit
AAACAAGGTCGTTTTTAAACTATTCGACACTAATTGATGGCCATCCGAAT1730.40483935132099313No Hit
GTCTCGCACCCGCGAGATACTCACAAAAACCACCGTGGACCATATGGCCA1680.3931387920342593No Hit
CTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAA1660.38845856831956566No Hit
ACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG1530.35803711417405754No Hit
ATCAAGAAGTACACATCAGGAAGACAGGAGAAGAACCCAGCACTTAGGAT1520.35569700231671075No Hit
GAAATATCCAATTACAGCAGACAAAGAAGACAAGAGATATGGGCCAGCAC1490.3486766667446704No Hit
GGCCAGCACTAAGCATCAATGAACTGAGCAACCTTGCGAAAGGAGAGAAG1420.3322958837432429No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAA1400.32761566002854936No Hit
GTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTA1380.3229354363138558No Hit
GCACTAAGCATCAATGAACTGAGCAACCTTGCGAAAGGAGAGAAGGCTAA1320.3088947651697751No Hit
GGATATTTCATTGCCATCATCCATTTCATCCTAAGTGCTGGGTTCTTCTC1310.3065546533124283No Hit
GTTCATTGATGCTTAGTGCTGGCCCATATCTCTTGTCTTCTTTGTCTGCT1260.2948540940256944No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACCACGT1230.2878337584536541No Hit
GGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATG1220.2854936465963073No Hit
AGTAGAAACAAGGTCGTTTTTAAACTATTCGACACTAATTGATGGCCATC1180.2761331991669202No Hit
GTGCTGGCCCATATCTCTTGTCTTCTTTGTCTGCTGTAATTGGATATTTC1170.2737930873095734No Hit
ATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCAGTC1160.27145297545222663No Hit
GCTTAGTGCTGGCCCATATCTCTTGTCTTCTTTGTCTGCTGTAATTGGAT1140.26677275173753306No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTT1010.2363512975920249No Hit
ATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACCACGTCTCCTTGCCC990.23167107387733135No Hit
CCATATGGCCATAATCAAGAAGTACACATCAGGAAGACAGGAGAAGAACC970.2269908501626378No Hit
AATTAGCACATTAGCCTTCTCTCCTTTCGCAAGGTTGCTCAGTTCATTGA960.22465073830529098No Hit
CATCCATTTCATCCTAAGTGCTGGGTTCTTCTCCTGTCTTCCTGATGTGT940.21997051459059747No Hit
TCGCAAGGTTGCTCAGTTCATTGATGCTTAGTGCTGGCCCATATCTCTTG940.21997051459059747No Hit
ACCCAGCACTTAGGATGAAATGGATGATGGCAATGAAATATCCAATTACA930.21763040273325066No Hit
GTAATTGGATATTTCATTGCCATCATCCATTTCATCCTAAGTGCTGGGTT930.21763040273325066No Hit
TCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCC910.2129501790185571No Hit
CATCAGGAAGACAGGAGAAGAACCCAGCACTTAGGATGAAATGGATGATG910.2129501790185571No Hit
GCAAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAAGA900.21061006716121033No Hit
CATTAGCCTTCTCTCCTTTCGCAAGGTTGCTCAGTTCATTGATGCTTAGT880.20592984344651677No Hit
ATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCAGTCTC860.2012496197318232No Hit
GTGCTGGGTTCTTCTCCTGTCTTCCTGATGTGTACTTCTTGATTATGGCC860.2012496197318232No Hit
GCCATATGGTCCACGGTGGTTTTTGTGAGTATCTCGCGGGTGCGAGACTG830.19422928415978283No Hit
CTTAGGGACAACCTTGAACCTGGGACCTTTGATCTTGGGGGGCTATATGA830.19422928415978283No Hit
GAGCAACCTTGCGAAAGGAGAGAAGGCTAATGTGCTAATTGGGCAAGGAG830.19422928415978283No Hit
GAGCGAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAA820.19188917230243605No Hit
GTGCTAATTGGGCAAGGAGACGTGGTGTTGGTAATGAAACGGAAACGGGA820.19188917230243605No Hit
GAAGTACACATCAGGAAGACAGGAGAAGAACCCAGCACTTAGGATGAAAT810.18954906044508926No Hit
CTATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGG790.1848688367303957No Hit
TCCAATTACAGCAGACAAAGAAGACAAGAGATATGGGCCAGCACTAAGCA790.1848688367303957No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATTCACTATTTT790.1848688367303957No Hit
GAACTAAGAAATCTAATGTCGCAGTCTCGCACCCGCGAGATACTCACAAA790.1848688367303957No Hit
CTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTCCG770.18018861301570216No Hit
ATTTCATCCTAAGTGCTGGGTTCTTCTCCTGTCTTCCTGATGTGTACTTC770.18018861301570216No Hit
TCTCTTGTCTTCTTTGTCTGCTGTAATTGGATATTTCATTGCCATCATCC750.1755083893010086No Hit
GGATGAAATGGATGATGGCAATGAAATATCCAATTACAGCAGACAAAGAA750.1755083893010086No Hit
GTACACATCAGGAAGACAGGAGAAGAACCCAGCACTTAGGATGAAATGGA730.17082816558631503No Hit
ATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTA720.16848805372896825No Hit
CTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTCCGTTTCATTACC710.16614794187162146No Hit
GCCATCATCCATTTCATCCTAAGTGCTGGGTTCTTCTCCTGTCTTCCTGA700.16380783001427468No Hit
ATATGAAGCAATTGAGGAGTGCCTGATTAATGATCCCTGGGTTTTGCTTA700.16380783001427468No Hit
CTAGCATACTTACTGACAGCCAGACAGCGACCAAAAGAATTCGGATGGCC700.16380783001427468No Hit
ATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCAGTCTCGCACC680.15912760629958111No Hit
TACCAACACCACGTCTCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTT660.15444738258488755No Hit
GAGAAGAACCCAGCACTTAGGATGAAATGGATGATGGCAATGAAATATCC660.15444738258488755No Hit
AATTACAGCAGACAAAGAAGACAAGAGATATGGGCCAGCACTAAGCATCA650.15210727072754077No Hit
AGCAAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAAG650.15210727072754077No Hit
AAATGGATGATGGCAATGAAATATCCAATTACAGCAGACAAAGAAGACAA640.14976715887019398No Hit
ATATGGGCCAGCACTAAGCATCAATGAACTGAGCAACCTTGCGAAAGGAG640.14976715887019398No Hit
ACCAACACCACGTCTCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTT640.14976715887019398No Hit
GATATGGGCCAGCACTAAGCATCAATGAACTGAGCAACCTTGCGAAAGGA630.1474270470128472No Hit
GGCTAATGTGCTAATTGGGCAAGGAGACGTGGTGTTGGTAATGAAACGGA630.1474270470128472No Hit
GAACTGAGCAACCTTGCGAAAGGAGAGAAGGCTAATGTGCTAATTGGGCA630.1474270470128472No Hit
GCTGTAATTGGATATTTCATTGCCATCATCCATTTCATCCTAAGTGCTGG630.1474270470128472No Hit
GCTTCATATAGCCCCCCAAGATCAAAGGTCCCAGGTTCAAGGTTGTCCCT630.1474270470128472No Hit
ACCTTGCGAAAGGAGAGAAGGCTAATGTGCTAATTGGGCAAGGAGACGTG630.1474270470128472No Hit
AAGAAATCTAATGTCGCAGTCTCGCACCCGCGAGATACTCACAAAAACCA620.14508693515550042No Hit
TTTCATTGCCATCATCCATTTCATCCTAAGTGCTGGGTTCTTCTCCTGTC620.14508693515550042No Hit
CTTCTTGATTATGGCCATATGGTCCACGGTGGTTTTTGTGAGTATCTCGC610.14274682329815366No Hit
TTCCTGATGTGTACTTCTTGATTATGGCCATATGGTCCACGGTGGTTTTT610.14274682329815366No Hit
AGATATGGGCCAGCACTAAGCATCAATGAACTGAGCAACCTTGCGAAAGG610.14274682329815366No Hit
TTACCAACACCACGTCTCCTTGCCCAATTAGCACATTAGCCTTCTCTCCT610.14274682329815366No Hit
GTTCTTCTCCTGTCTTCCTGATGTGTACTTCTTGATTATGGCCATATGGT580.13572648772611332No Hit
CTCTTGTCTTCTTTGTCTGCTGTAATTGGATATTTCATTGCCATCATCCA570.13338637586876653No Hit
GCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTC560.13104626401141975No Hit
CACCACGTCTCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTCGCAA560.13104626401141975No Hit
GGAGAAGAACCCAGCACTTAGGATGAAATGGATGATGGCAATGAAATATC560.13104626401141975No Hit
AACTAACTCAATGCATGTGTAAGGAAGGAGTTGAACCAAGAAGCATTAAG560.13104626401141975No Hit
ATCCAATTACAGCAGACAAAGAAGACAAGAGATATGGGCCAGCACTAAGC540.12636604029672618No Hit
CACAAAAACCACCGTGGACCATATGGCCATAATCAAGAAGTACACATCAG540.12636604029672618No Hit
GGCCCATATCTCTTGTCTTCTTTGTCTGCTGTAATTGGATATTTCATTGC530.1240259284393794No Hit
CTTACTGACAGCCAGACAGCGACCAAAAGAATTCGGATGGCCATCAATTA530.1240259284393794No Hit
GCCACAACTAACTCAATGCATGTGTAAGGAAGGAGTTGAACCAAGAAGCA530.1240259284393794No Hit
ATTAGCACATTAGCCTTCTCTCCTTTCGCAAGGTTGCTCAGTTCATTGAT530.1240259284393794No Hit
GATGTGTACTTCTTGATTATGGCCATATGGTCCACGGTGGTTTTTGTGAG530.1240259284393794No Hit
GTATGGGGAGGACCTGAAAATCGAAACAAACAAATTTGCAGCAATATGCA530.1240259284393794No Hit
AGCGAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTT530.1240259284393794No Hit
TAGTAGCACTGCCACAACTAACTCAATGCATGTGTAAGGAAGGAGTTGAA520.12168581658203262No Hit
GTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTCCGTTTCATTACCAA520.12168581658203262No Hit
GCAGAAGGATTTTCAGCTGAATCAAGAAAACTGCTTCTTATCGTTCAGGC510.11934570472468584No Hit
TGCGAAAGGAGAGAAGGCTAATGTGCTAATTGGGCAAGGAGACGTGGTGT500.11700559286733905No Hit
TAATCAAGAAGTACACATCAGGAAGACAGGAGAAGAACCCAGCACTTAGG500.11700559286733905No Hit
GCATTAAGCAAAACCCAGGGATCATTAATCAGGCACTCCTCAATTGCTTC500.11700559286733905No Hit
GTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCACT490.11466548100999227No Hit
ATATGGCCATAATCAAGAAGTACACATCAGGAAGACAGGAGAAGAACCCA490.11466548100999227No Hit
TCATTAATCAGGCACTCCTCAATTGCTTCATATAGCCCCCCAAGATCAAA490.11466548100999227No Hit
TCGCAGTCTCGCACCCGCGAGATACTCACAAAAACCACCGTGGACCATAT480.11232536915264549No Hit
CCTTTCGCAAGGTTGCTCAGTTCATTGATGCTTAGTGCTGGCCCATATCT470.10998525729529873No Hit
ACCGTGGACCATATGGCCATAATCAAGAAGTACACATCAGGAAGACAGGA470.10998525729529873No Hit
AAGTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACCACGTCTCCTT470.10998525729529873No Hit
CAATTATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCG470.10998525729529873No Hit
ACCTTGAACCTGGGACCTTTGATCTTGGGGGGCTATATGAAGCAATTGAG460.10764514543795195No Hit
TCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTCGCAAGGTTGCTCA460.10764514543795195No Hit
CACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGT460.10764514543795195No Hit
TATCTCTTGTCTTCTTTGTCTGCTGTAATTGGATATTTCATTGCCATCAT460.10764514543795195No Hit
TTAGCCTTCTCTCCTTTCGCAAGGTTGCTCAGTTCATTGATGCTTAGTGC460.10764514543795195No Hit
CCCGCGAGATACTCACAAAAACCACCGTGGACCATATGGCCATAATCAAG450.10530503358060517No Hit
CCCATATCTCTTGTCTTCTTTGTCTGCTGTAATTGGATATTTCATTGCCA450.10530503358060517No Hit
GAAGACAAGAGATATGGGCCAGCACTAAGCATCAATGAACTGAGCAACCT450.10530503358060517No Hit
GGGCAAGGAGACGTGGTGTTGGTAATGAAACGGAAACGGGACTCTAGCAT450.10530503358060517No Hit
GCATCAATGAACTGAGCAACCTTGCGAAAGGAGAGAAGGCTAATGTGCTA440.10296492172325838No Hit
GAAATGGATGATGGCAATGAAATATCCAATTACAGCAGACAAAGAAGACA440.10296492172325838No Hit
GTGTACTTCTTGATTATGGCCATATGGTCCACGGTGGTTTTTGTGAGTAT440.10296492172325838No Hit
AGCCTTCTCTCCTTTCGCAAGGTTGCTCAGTTCATTGATGCTTAGTGCTG440.10296492172325838No Hit
AGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCAC440.10296492172325838No Hit
GGATGATGGCAATGAAATATCCAATTACAGCAGACAAAGAAGACAAGAGA430.1006248098659116No Hit
GACGTGGTGTTGGTAATGAAACGGAAACGGGACTCTAGCATACTTACTGA430.1006248098659116No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGATG100.0070672766145.000024
AAGGGTG100.0070672766145.000025
AGATGGT100.0070672766145.000026
AGGCAGA100.0070672766145.000026
CTGCTTA202.1070846E-6145.00002145
TAGGCAG100.0070672766145.000025
AGCCCCG151.2218393E-4145.0145
GAGCGAA256.393704E-6116.01
ATAAGAT203.8404833E-4108.750013
AATAAGA203.8404833E-4108.750012
GAGCAAA1150.0107.173921
GCAAAAG1150.0107.173923
CTCTTAA352.7163514E-7103.57143145
AGTAGAA501.891749E-10101.51
CAAAAGC1250.098.64
TATGGAT451.0666554E-896.6666642
CGAAAGC651.4551915E-1189.230773
AGCGAAA651.4551915E-1189.230771
GGTTGCC259.3248853E-487.0145
GGATAGC502.2168024E-887.05
Produced by FastQC (version 0.11.5)