FastQCFastQC Report
Mon 4 Feb 2019
000000000-BTF83_l01_n01_Exp2_SrcID29_Shed_Ind2_D1_rep2_SID60.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BTF83_l01_n01_Exp2_SrcID29_Shed_Ind2_D1_rep2_SID60.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences100677
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA2230.22150044200760852No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT1740.1728299412974165No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG1690.16786356367392752No Hit
AAACAAGGGTGTTTTTTATTATTAAATAAGCTGAAACGAGAAAGTTCTTA1530.15197115527876276No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA1410.14005184898238923No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT1400.13905857345769143No Hit
CTCCAGCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCACTCT1390.13806529793299363No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGATCCCCGTTCCC1330.13210564478480685No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC1330.13210564478480685No Hit
TCCTAGCTCCAGTGCTGGTCTGAAAAATGATCTTCTTGAAAATTTGCAGG1280.12713926716131788No Hit
GTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGATGTT1260.1251527161119223No Hit
ATATACAACAGGATGGGGGCTGTGACCACTGAAGTGGCATTTGGCCTGGT1230.1221728895378289No Hit
CTCTAATATTGCTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAA1230.1221728895378289No Hit
GTATACATCATGGTCATAAGTCCCATTTCTGATTGACTCTATGCAAGCGT1190.11819978743903771No Hit
CTACTAGACAGACCCCTTACCCAGGGTCTGGACCCGATATTCGGGATTAT1150.11422668534024653No Hit
TGATAACACAGTTCGAGTCTCTGAAACTCTACAGAGATTCGCTTGGAGAA1120.11124685876615313No Hit
GTGTTATCATTCCATTCAAGTCCCCCGATGAGGACTCCAACTGCATTTTT1110.11025358324145534No Hit
GATGTATACAGAGACGAAGCATTAAACAACCGGTTTCAGATCAAAGGTGT1100.10926030771675754No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTATTC100.007081458145.0145
GGCAAAC100.007081458145.03
CCGTACA100.007081458145.0145
CTGCTTA458.367351E-11112.77778145
GGATCCG404.771209E-9108.75145
CCCCGGC203.8558518E-4108.75145
AGCGAAA553.6379788E-12105.454551
GTAGCAC259.362063E-487.03
CGAAAGC755.4569682E-1177.333333
GCACTGC300.001930670672.56
TAGTAGC300.001930670672.51
CTCGTAG350.003557193662.142857145
CAAGGTA350.003557193662.1428579
GCAGGGT702.313318E-762.1428579
ACAAGGT400.00603514954.3758
GTAGAAA858.844181E-751.1764681
ACTGCCA450.0096141648.3333328
GGTCCCT450.0096141648.333332145
CACTGCC450.0096141648.3333327
TAGCACT450.0096141648.3333324
Produced by FastQC (version 0.11.5)