FastQCFastQC Report
Mon 4 Feb 2019
000000000-BTF83_l01_n01_Exp2_SrcID28_Shed_Ind1_D1_rep2_SID59.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BTF83_l01_n01_Exp2_SrcID28_Shed_Ind1_D1_rep2_SID59.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences136232
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT2940.21580832697163663No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA2920.21434024311468675No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG2540.18644664983263845No Hit
AAACAAGGGTGTTTTTTATTATTAAATAAGCTGAAACGAGAAAGTTCTTA2420.177638146690939No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA2380.17470197897703918No Hit
ATATACAACAGGATGGGGGCTGTGACCACTGAAGTGGCATTTGGCCTGGT2310.16956368547771447No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2190.16075518233601502No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC2160.15855305655059018No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT2120.15561688883669036No Hit
CTCCAGCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCACTCT1950.1431381760526161No Hit
GTGTTATCATTCCATTCAAGTCCCCCGATGAGGACTCCAACTGCATTTTT1900.13946796641024134No Hit
CTCTAATATTGCTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAA1830.1343296729109167No Hit
GGTATGTGCAACCTGTGAACAGATTGCTGACTCCCAGCATCGGTCTCATA1800.13212754712549182No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGATCCCCGTTCCC1770.12992542134006693No Hit
TCCTAGCTCCAGTGCTGGTCTGAAAAATGATCTTCTTGAAAATTTGCAGG1680.12331904398379236No Hit
TGATAACACAGTTCGAGTCTCTGAAACTCTACAGAGATTCGCTTGGAGAA1670.1225850020553174No Hit
GTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGATGTT1620.11891479241294262No Hit
GTATACATCATGGTCATAAGTCCCATTTCTGATTGACTCTATGCAAGCGT1570.11524458277056784No Hit
TCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGATCCCCGTTCCCA1530.11230841505666803No Hit
GATGTATACAGAGACGAAGCATTAAACAACCGGTTTCAGATCAAAGGTGT1490.10937224734276821No Hit
CTACTAGACAGACCCCTTACCCAGGGTCTGGACCCGATATTCGGGATTAT1460.10717012155734337No Hit
CTCTCTATCATCCCGTCAGGCCCCCTCAAAGCCGAGATCGCACAGAGACT1450.10643607962886839No Hit
AGCGAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAGC1390.10203182805801868No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCTC151.2276918E-4145.0145
CTATACG100.007084187145.0145
GAGCGAA202.1239357E-6145.01
AACGCGC100.007084187145.0145
AGCAGGC100.007084187145.08
CTGCTTA301.594479E-596.666664145
GCGGTCG259.3692215E-487.08
AAGGTCG259.3692215E-487.09
TCGTACT259.3692215E-487.02
TCTCCGC259.3692215E-487.0145
CGGTCGG259.3692215E-487.09
CATTTCG259.3692215E-487.0145
TTTCCTA353.4266042E-582.85714145
GTAGAAA1450.075.01
CTGTAGG300.001932141672.5145
AGCCCCG502.2955446E-672.5145
CAAGGTC300.001932141672.58
TATAGCG300.001932141672.52
ACAAGGT502.2955446E-672.57
AGCGAAA651.3890167E-766.923071
Produced by FastQC (version 0.11.5)