FastQCFastQC Report
Mon 4 Feb 2019
000000000-BTF83_l01_n01_Exp2_Neg_control_Water_SID76.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BTF83_l01_n01_Exp2_Neg_control_Water_SID76.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTCAATCC33.75No Hit
ATCTAATGTCGCAGTCTCGCACCCGCGAGATACTCACAAAAACCACCGTG22.5No Hit
TTCTTTATCCTTCCAGATTCGAAATCAATCCGTGCATCAATTCGGGCTCT22.5No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGCGGGGGGGGG22.5No Hit
ATATGAGTGCGACTCCCCTGCGAGCAACCAAGTAATGCCGTGTGAACCAA11.25No Hit
GTTCATACAGAAGACCAGTCGGGATATCCAGTATGGTGGAGGCTATGGTT11.25No Hit
ATGGAAACCAACCTGCTGCAACAGCATTGGCCAACACAATAGAAGTGTTC11.25No Hit
GTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACCACGTCTCCTTGC11.25No Hit
ATCCACGGCTTGCTCTTCTGTTGGGTTCTGCCTAAGGATGTCTACCATCC11.25No Hit
TTTCCTTATCCTTCCAGATTCGAAATCAATCCGTGCATCAATTCGGGCTC11.25No Hit
ATTCCATTCAAGTCCCCCGATGAGGACTCCAACTGCATTTTTGACATCCT11.25No Hit
ATGCTGGGAGTCAGCAATCTGTTCACAGGTTGCACATACCAGGCCAAATG11.25No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT11.25No Hit
TTTTCAGCTGAATCAAGAAAACTGCTTCTTATCGTTCAGGCTCTTAGGGA11.25No Hit
ATCATGAAGGGATTCAAGACGGAGTCGACAGGTTTTATCGAACCTGTAAG11.25No Hit
GATAATAAAACTTCTTCCCTTCGCAGCCGCTCCACCAAAGCAAAGTAGAA11.25No Hit
CTATACAGTTTAACTGCTTTGTCCATGTTATTTGGATCCCCGTTCCCATT11.25No Hit
TGTATCAATGGGACTTGCACAGTAGTAATGACTGATGGAAGTGCTTCAGG11.25No Hit
ATTAGTAGAAACAAGGGTGTTTTTCCTCATATTTCTGAAATTCTAATCTC11.25No Hit
TGTCAATCCGACCTTACTTTTCTTAAAAGTGCCAGCACAAAATGCTATAA11.25No Hit
TCTTATAAGCATATCTCCTATCTCAAGAACACAGTACTTCTCCCATTTAT11.25No Hit
GTATGCTTCCTCATGCAGATTGCCATCCTGGTAACTACTGTAACATTGCA11.25No Hit
TGGAGTGAGTGGAGGTCTCCCATTCTCATTACTGCTTCTCCAAGCGAATC11.25No Hit
CAGTATGCTTCCTCATGCAGATTGCCATCCTGGTAACTACTGTAACATTG11.25No Hit
AATGTTGCCTCTCTGGCAGGCCCACATGATGAACCCCAGCAAAACAACAC11.25No Hit
ATTTTCAGCTGAATCAAGAAAACTGCTTCTTATCGTTCAGGCTCTTAGGG11.25No Hit
TTCCTTAAGTGGGATCTTCCTTTTATGATGAAACCATACAAGTTGGTCTT11.25No Hit
GAAGAGTTCACTGAGATCATGAAGATCTGTTCCACCATTGAAGAGCCCAG11.25No Hit
TGTAAGGAAGGAGTTGAACCAAGAAGCATTAAGCAAAACCCAGGGATCAT11.25No Hit
ATAAAGTCCTGCAGACCTTCCCAATGGAACTTTCCTCCACTCCTTTGGGG11.25No Hit
CTCCTGGATCCCCAAAAGAAATCGATCCATCTTGAATACAAGTCAAAGAG11.25No Hit
GACTGAAAGGAGGGCCTTCTACGGAAGGAGTGCCAAAGTCTATGAGGGCA11.25No Hit
ATGTCCTGGAAGAGAAGGCAATGGTGAAATTTCGCCAACAATTGCTCCCT11.25No Hit
GAGCGAAAGCAGGGGAGTGAGGGGTTGTGTGTGTGGGGGAAGTGTGATCA11.25No Hit
CTATCAGCTGAATCAATCTCCTGGTTGCTTTTCTGAGTATGGCTGTTGCT11.25No Hit
CCTAAGAGGAAGGGGCAGCACCCTCGGTCTGGACATCGAGACAGCCACAC11.25No Hit
GTATTTGCAGCACTACAGGGGCTGAGAAACCAAAGTTTCTACCAGATTTG11.25No Hit
AAATGAAAGAGTATGGGGAGGACCTGAAAATCGAAACAAACAAATTTGCA11.25No Hit
AGCGAAAGCAGGGTAGATAATCACTCACTGAGTGACATCAAAATCATGGC11.25No Hit
GCCACAACTAACTCAATGCATGTGTAAGGAAGGAGTTGAACCAAGAAGCA11.25No Hit
GCACAACTTTCCCTTATACTGGAGACCCTCCTTACAGCCATGGGACAGGA11.25No Hit
GCATGGAGTATGATGCTGTTGCAACAACACACTCCTGGATCCCCAAAAGA11.25No Hit
TGACTCTTGAGGAAATGTCAAGGGACTGGTCCATGCTCATACCCAAGCAG11.25No Hit
ATGCATGTGTAAGGAAGGAGTTGAACCAAGAAGCATTAAGCAAAACCCAG11.25No Hit
CCTCCTAGGGAGAGTTGAACCTTGCATCAGAGAGCACATCCTGGGATCCA11.25No Hit
CATATGGAGCATGCCCCAAGTATGTTAAGCAAAACACCCTGAAGTTGGCA11.25No Hit
TTGTTCAGCAAACACGAGTAGACAAGCTGACACAAGGCCGACAGACCTAT11.25No Hit
CCATGTCAACAGGACACATCAGTACTCAGAAAAGGGAAGATGGACAACAA11.25No Hit
TAAGGAGGGTCTCCAGTATAAGGGAAAGTTGTGCTTATAAGAAAAGTAAG11.25No Hit
TTTGATCTGAAACCGGTTGTTTAATGCTTCGTCTCTGTATACATCATGGT11.25No Hit
GAGGCAATCTCGTATGCCGTCTTCTGCTTGAAAATAAACACCTCACATAC11.25RNA PCR Primer, Index 15 (96% over 28bp)
ATATTCTCATTGGAAGCAATTTGAACTCCTTTAGTGGAAAGCTTCCCTCT11.25No Hit
AACAAGAGCTCTTGACCTCTGATAAGTTGCATCATTCAAATTGGAATGCC11.25No Hit
AGCGAAAGCAGGTACTGATCCAAAATGTCAGATTTTGTGCGACAATGCTT11.25No Hit
GTCTTTAGCCATTCCATGAGAACCTCAAGATCAGTGTTCTTCCTGTCTCT11.25No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGAGGCGGGG11.25No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGAC11.25No Hit
TTCTGGTGCCATGTTCTCACCAAGTGCCCACTTTAGCTGACTTGTTTTCT11.25No Hit
GGCTAATGTGCTAATTGGGCAAGGAGACGTGGTGTTGGTAATGAAACGGA11.25No Hit
CTATAAGGGCTTCTGTCCTTAACAGTCCCATTTGAATGCTTGTCATTCCT11.25No Hit
GAACTGGTTGACCAAATCAGGAAGCACATATCCAGTGCTGAACGTGACAA11.25No Hit
CTTCTTTCTTTATCCTTCCAGATTCGAAATCAATCCGTGCATCAATTCGG11.25No Hit
GTTCCATGGTGTATCCTGTTCCTGTCCCATGGCTGTAAGGAGGGTCTCCA11.25No Hit
CTTGAGGAAATGTCAAGGGACTGGTCCATGCTCATACCCAAGCAGAAAGT11.25No Hit
GAGCAAAAGCAGGGGCGGTGTGGGGGAAGGGAAGCTCTGAGGGTTCCCGA11.25No Hit
GATCCAAATAACATGGACAAAGCAGTTAAACTGTACAGGAAGCTCACGAG11.25No Hit
ATAGAGAGAATGGTGCTCTCTGCTTTTGACGAAAGGAGAAATAAATACCT11.25No Hit
AGTAATGACTGATGGAAGTGCTTCAGGAAGAGCCGATACTAGAATACTAT11.25No Hit
GTCTTTCTCTTTGACAGAGGACTCAGCTTCAATCGAGTGCATATTGCTGC11.25No Hit
GAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTT11.25No Hit
GTATTCAACAGCTTGTGGGGGGCTATATGAAGCAATTGAGGAGTGCCTGA11.25No Hit
GATAACACAGTTCGAGTCTCTGAAACTCTACAGAGATTCGCTTGGAGAAG11.25No Hit
GATTTGAAATAATCGAGGGAAGAGATCGCACAATGGCCTGGACAGTAGTA11.25No Hit
TCTAATGTCGCAGTCTCGCACCCGCGAGATACTCACAAAAACCACCGTGG11.25No Hit
GTGTAAGGAAGGAGTTGAACCAAGAAGCATTAAGCAACACCTAGGGATCA11.25No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGAC50.0145.08
CAATGGG50.0145.06
GTATCAA50.0145.02
TCAATGG50.0145.05
TTTTATC50.0145.0145
AATGGGA50.0145.07
TATCAAT50.0145.03
ATCAATG50.0145.04
TGGGACT50.0145.09
TGTATCA50.0145.01