FastQCFastQC Report
Mon 4 Feb 2019
000000000-BTF83_l01_n01_Exp2_IAV_control_SID75.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BTF83_l01_n01_Exp2_IAV_control_SID75.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences145136
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA5330.3672417594532025No Hit
GAGCAAAAGCAGGGGTTTAAAATGAATCCAAATCAGAAAATAACAACCAT5170.35621761658031087No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT4400.3031639290045199No Hit
GTCCTTAACAGTCCCATTTGAATGCTTGTCATTCAGTAAGGCACCTTGGG3970.2735365450336236No Hit
GTCTAGCCCTGTTAGCTCAGGATGTTGAACGAAACTTCCGCTATACCCTG3810.262512402160732No Hit
GTATATAGCCCACCCACGGATGGGACAAAGAGATGAATTGCCGGTTAATA3700.254933303935619No Hit
GTTAAGGACAGAAGCCCTTATAGGGCCTTAATGAGCTGCCCTGTCGGTGA3660.25217726821739606No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA3630.2501102414287289No Hit
GGTATGGTAATGGTGTTTGGATAGGAAGGACCAAAAGTCACAGTTCCAGA3570.24597618785139455No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG3370.23219600926028No Hit
ATATACAGCAAAGACAATAGCATAAGAATTGGTTCCAAAGGAGACGTTTT3350.23081799140116854No Hit
ATATACAACAGGATGGGGGCTGTGACCACTGAAGTGGCATTTGGCCTGGT2780.1915444824164921No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2770.1908554734869364No Hit
TCCTAGCTCCAGTGCTGGTCTGAAAAATGATCTTCTTGAAAATTTGCAGG2630.1812093484731562No Hit
TTCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGATCCCCGTTCCC2610.17983133061404477No Hit
CTCCAGCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCACTCT2460.16949619667070887No Hit
ATATAGCCCACCCACGGATGGGACAAAGAGATGAATTGCCGGTTAATATC2330.1605390805864844No Hit
TTTTTGAACAGACTACTTGTCAATGCTGAATGGCAACTCAGCACCGTCTG2290.15778304486826147No Hit
GTATTAAAATACAACGGCATAATAACTGAAACCATAAAAAGTTGGAGGAA2290.15778304486826147No Hit
GCTGTATATAGCCCACCCACGGATGGGACAAAGAGATGAATTGCCGGTTA2280.15709403593870577No Hit
TACCATACCTATATGAAAATCCCTTTACTCCGTTTGCTCCATCAACATAC2220.1529599823613714No Hit
GGGCTAGACTGTATGAGGCCGTGCTTCTGGGTTGAATTAATCAGGGGACG2190.15089295557270424No Hit
CAATATGGATTAGCCATTCAATTCAAACTGGAAGTCAAAACCATACTGGA2170.14951493771359275No Hit
GGATTAGCCATTCAATTCAAACTGGAAGTCAAAACCATACTGGAATATGC2140.14744791092492557No Hit
GCCTCATACAGTCTAGCCCTGTTAGCTCAGGATGTTGAACGAAACTTCCG2120.14606989306581414No Hit
GTATGAGGCCGTGCTTCTGGGTTGAATTAATCAGGGGACGACCTAAAGAA2070.1426248484180355No Hit
GACTACTTGTCAATGCTGAATGGCAACTCAGCACCGTCTGGCCAAGACCA1990.1371127769815897No Hit
AAGTAATAGTGACTAAGTAAAGGGCTTTCACCGAAGAGGGAGCAATTGTT1870.12884466982692094No Hit
GTGTAAATGGTTCATGTTTTACTATAATGACTGATGGCCCGAGTGATGGG1850.1274666519678095No Hit
GGTATGTGCAACCTGTGAACAGATTGCTGACTCCCAGCATCGGTCTCATA1810.1247106162495866No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC1780.12264358946091941No Hit
TCCTATACAGTTTAACTGCTTTGTCCATGTTATTTGGATCCCCGTTCCCA1780.12264358946091941No Hit
GGAATATGCAACCAAAACATCATTACCTATAAAAATAGCACCTGGGTAAA1770.12195458053136368No Hit
GCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCACTCTGCTGT1740.1198875537426965No Hit
GTGTTATCATTCCATTCAAGTCCCCCGATGAGGACTCCAACTGCATTTTT1720.11850953588358505No Hit
GGATACATCTGCAGTGGGGTTTTCGGTGACAACCCGCGTCCCGAAGATGG1720.11850953588358505No Hit
GCCTAATATTGCAAATAGGGAATATAATCTCAATATGGATTAGCCATTCA1690.11644250909491786No Hit
CTCTATCATCCCGTCAGGCCCCCTCAAAGCCGAGATCGCACAGAGACTTG1670.11506449123580642No Hit
ACATACACTGGACCACAGCTGCCTGTTCCATCTTCGGGACGCGGGTTGTC1660.11437548230625069No Hit
ATCAAATAGGATACATCTGCAGTGGGGTTTTCGGTGACAACCCGCGTCCC1650.11368647337669496No Hit
ACTCAGCACCGTCTGGCCAAGACCAATCTACAGTATCACTATTCACGCCA1640.11299746444713925No Hit
CCCATGGCCGGTTCGAACCATGCCAGTTGTCTCTGCACACACACATCACT1630.1123084555175835No Hit
AAACAAGGGTGTTTTTTATTATTAAATAAGCTGAAACGAGAAAGTTCTTA1630.1123084555175835No Hit
GGCACATTCAGACTCTTGTGTCCTCAATATTTTCTTCCTCCAACTTTTTA1620.11161944658802778No Hit
TTTTAATACAGCCACTGCTCCATTATCTGGACCTGAAATTCCGATTGTTA1610.11093043765847206No Hit
GTGTATGTTGATGGAGCAAACGGAGTAAAGGGATTTTCATATAGGTATGG1610.11093043765847206No Hit
GTATCACTATTCACGCCACAAAAAGAAATGCTGCTCGCACTAGTCCAGAT1590.10955241979936059No Hit
TTACCATACCTATATGAAAATCCCTTTACTCCGTTTGCTCCATCAACATA1570.10817440194024915No Hit
CTCTCTATCATCCCGTCAGGCCCCCTCAAAGCCGAGATCGCACAGAGACT1560.10748539301069342No Hit
CATCTGCAGTGGGGTTTTCGGTGACAACCCGCGTCCCGAAGATGGAACAG1560.10748539301069342No Hit
GCACATACCAGGCCAAATGCCACTTCAGTGGTCACAGCCCCCATCCTGTT1560.10748539301069342No Hit
GCAAAAGCAGGGGTTTAAAATGAATCCAAATCAGAAAATAACAACCATTG1560.10748539301069342No Hit
GTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGAGTG1560.10748539301069342No Hit
CTATCATCCCGTCAGGCCCCCTCAAAGCCGAGATCGCACAGAGACTTGAA1540.10610737515158197No Hit
GGGTAAAGGACACAACTTCAGTGATATTAACCGGCAATTCATCTCTTTGT1530.10541836622202623No Hit
GAAGAACACTGATCTTGAGGTTCTCATGGAATGGCTAAAGACAAGACCAA1530.10541836622202623No Hit
GTCTCTGTCCATCCATTAGGATCCCAAATCATCTCAAACCCATGTCTGGA1490.10266233050380331No Hit
GTTCCAGACATGGGTTTGAGATGATTTGGGATCCTAATGGATGGACAGAG1490.10266233050380331No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGGCG100.007084661145.0145
CCGCTAC100.007084661145.0145
GAGCGAA351.0913936E-11145.01
CTCGTAG301.0969234E-7120.833336145
TCTACTA256.4461183E-6116.05
TCTCCGC256.4461183E-6116.0145
CGAGACG256.4461183E-6116.0145
CCGTATC404.785761E-9108.75145
CTCCGAC1000.0101.5145
GCAAAAG3150.0101.2698443
CTGCTTA406.090977E-790.625145
GCAGGGG1600.090.6259
CTATGAA259.370467E-487.0145
GGATCCG259.370467E-487.0145
CAAAAGC3700.084.256764
AGCAAAA3750.083.133322
CTACTAA353.4273624E-582.857146
GGATAGC353.4273624E-582.857145
TACTAAT353.4273624E-582.857147
GATAGCA353.4273624E-582.857146
Produced by FastQC (version 0.11.5)