FastQCFastQC Report
Mon 4 Feb 2019
000000000-BTF83_l01_n01_Exp1_SrcID9_Shed_Ind2_D3_rep1_SID9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BTF83_l01_n01_Exp1_SrcID9_Shed_Ind2_D3_rep1_SID9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39824
Sequences flagged as poor quality0
Sequence length151
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTT4961.245480112494978No Hit
CAGTATCCTTCCAGATTCGAAATCAATCCGTGCATCAATTCGGGCTCTGG3950.9918642024909602No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT2130.5348533547609482No Hit
AGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTCAATCC1530.3841904379268783No Hit
GGATATTTGAAAAATTCTTCCCCAGCAGTTCATACAGAAGACCAGTCGGG1500.3766572920851748No Hit
GTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTA1280.32141422257934915No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG1260.3163921253515468No Hit
CAGTGAACTCTTCTTTCTTTATCCTTCCAGATTCGAAATCAATCCGTGCA1210.303836882282041No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA1170.2937926878264363No Hit
GAGCAAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAA1140.2862595419847328No Hit
ATACTGGATATCCCGACTGGTCTTCTGTATGAACTGCTGGGGAAGAATTT1140.2862595419847328No Hit
GGATACACCATGGATACTGTCAACAGGACACATCAGTACTCAGAAAAGGG1140.2862595419847328No Hit
GAGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTT1070.2686822016874246No Hit
CCTCTTTGACTTGTCTGGCAGTGGCCCATCAATCGGGTTGAGTTGCGGTG1010.2536159100040177No Hit
GAGCAAAAGCAGGGTAGATAATCACTCACTGAGTGACATCAAAATCATGG1000.2511048613901165No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATTCACTATTTT1000.2511048613901165No Hit
CCCTTATACTGGAGACCCTCCTTACAGCCATGGGACAGGAACAGGATACA960.24106066693451184No Hit
GAAGCAGTTTTCTTGATTCAGCTGAAAATCCTTCTAGTTGTGGAGATGCA900.22599437525110486No Hit
ATACTGGAGACCCTCCTTACAGCCATGGGACAGGAACAGGATACACCATG890.22348332663720372No Hit
GAACAAAGGTGCTGCAATTTATTTGAAAAATTCTTCCCCAGCAGTTCATA870.21846122940940138No Hit
GTCTGGCAGTGGCCCATCAATCGGGTTGAGTTGCGGTGCTCCAGTTTCGG840.21092808356769788No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAA810.2033949377259944No Hit
CCTTACAGCCATGGGACAGGAACAGGATACACCATGGATACTGTCAACAG680.17075130574527922No Hit
TCTCAGTGAACTCTTCTTTCTTTATCCTTCCAGATTCGAAATCAATCCGT680.17075130574527922No Hit
CATCAGTACTCAGAAAAGGGAAGATGGACAACAAACACCGAAACTGGAGC630.1581960626757734No Hit
GCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTTTC630.1581960626757734No Hit
GAGCGAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT560.14061872237846523No Hit
GAGCAAAAGCAGGAGTGAAAATGAATCCAAATCAAAAGATAATAACAATT550.13810767376456407No Hit
CTCAGTGAACTCTTCTTTCTTTATCCTTCCAGATTCGAAATCAATCCGTG540.13559662515066292No Hit
GTATGAACTGCTGGGGAAGAATTTTTCAAATAAATTGCAGCACCTTTGTT530.13308557653676176No Hit
ATCCAGTATGGTGGAGGCTATGGTTTCCAGAGCCCGAATTGATGCACGGA510.12806347930895942No Hit
ATATACAACAGGATGGGGGCTGTGACCACTGAAGTGGCATTTGGCCTGGT510.12806347930895942No Hit
CAAACAAATTTGCAGCAATATGCACTCACTTGGAAGTATGCTTCATGTAT510.12806347930895942No Hit
GTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGATGTT510.12806347930895942No Hit
ATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTATGA500.12555243069505825No Hit
CCTTTGTTCATCTTCAAGTACTCCTCTTTGACTTGTCTGGCAGTGGCCCA500.12555243069505825No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGTATCCTTCCAGATTCGA490.12304138208115709No Hit
CACTTGGAAGTATGCTTCATGTATTCAGATTTTCACTTCATCAATGAGCA480.12053033346725592No Hit
TTTTTAAACTATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG480.12053033346725592No Hit
CCTGTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGAT480.12053033346725592No Hit
CATGTGGAGTTGATAATATGCACTCACTTGGAAGTATGCTTCATGTATTC460.1155082362394536No Hit
ATTATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCA460.1155082362394536No Hit
CCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATGTCAAAG460.1155082362394536No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG450.11299718762555243No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT440.11048613901165126No Hit
AGCGAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTTT440.11048613901165126No Hit
CATCTTCAAGTACTCCTCTTTGACTTGTCTGGCAGTGGCCCATCAATCGG430.10797509039775009No Hit
GTATTCATCTCCACAACTAGAAGGATTTTCAGCTGAATCAAGAAAACTGC420.10546404178384894No Hit
TAGTAGCACTGCCACAACTAACTCAATGCATGTGTAAGGAAGGAGTTGAA420.10546404178384894No Hit
CCATACTGGATATCCCGACTGGTCTTCTGTATGAACTGCTGGGGAAGAAT420.10546404178384894No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGCTAAATTCACTATTTTTGCCG410.10295299316994777No Hit
GTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACCACGTCTCCTTGC410.10295299316994777No Hit
AGCGAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAAG410.10295299316994777No Hit
ATACATGATAGAATCCCTCATCGAACCCTATTAATGAATGAGTTGGGTGT400.1004419445560466No Hit
GAGCAAAAGCAGGGGATAATTCTATTAATCATGAAGACCATCATTGCTTT400.1004419445560466No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC400.1004419445560466No Hit
CCATTTGAATGGATGTCAATCCGACCTTACTTTTCTTAAAAGTGCCAGCA400.1004419445560466No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAG100.007065483145.00002145
GTACTAA100.007065483145.00002145
ATACGGC100.007065483145.00002145
GAGCAAA2350.0120.319151
GCAGGAG203.838541E-4108.750019
AGCAGGC1200.0108.758
GCAAAAG2800.0103.571433
AAGCAGG3100.098.225817
CAAAAGC3100.093.5483864
AGCCCAG259.3201856E-487.0145
GCAGGCA1500.087.09
AGCAGGA259.3201856E-487.08
AAAGCAG3550.085.774656
AGCAAAA3400.083.1617662
AAAAGCA3850.075.324675
AGCAGGT1350.075.185198
GCAGGGT607.834933E-872.59
AGCAGGG702.7212081E-972.58
GCAGGTC451.1798812E-464.444449
GGATAGC350.003541462.1428575
Produced by FastQC (version 0.11.5)